HB101 Reagent – Frozen Liquid

HB101 strain chemically competent cells from Molecular Innovations are an all purpose reagent for general cloning, sub-cloning and plasmid isolation. For a transformation efficiency of 3×106 simply thaw a tube of cells on ice, add 1-5 ul of DNA, gently mix and incubate on ice for 2-5 minutes, then spread 50-100 ul onto a pre-warmed culture plate. For greater transformation efficiencies heat shock at 42C for 30 seconds then add SOC media and shake at 225 rpm at 37C for 1 hour before plating. Conveniently supplied as a pack of 10 convenient single use 0.1 ml aliquots.

Small volumes of HB101 reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS136042 is a reagent targeting HB101. Also known as Mix and Go Competent Cells Strain HB101. Host: E Coli.