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Hoechst 33342 Reagent

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS826727

MBS826727 is a reagent targeting Hoechst 33342. Also known as Hoechst 33342 Staining Kit. Hoechst 33342 Staining Kit More details

$170 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Hoechst 33342, Reagent
Full Product Name
Hoechst 33342 Staining Kit
Preparation and Storage
Store at 4 degree C.
Applications Tested/Suitable for Hoechst 33342 reagent
Immunofluorescence (IF)
Application Notes for Hoechst 33342 reagent
Hoechst 33342 is a cell permeable fluorescent minor groove-binding probe for DNA, and specific stain for AT-rich regions of double-stranded DNA. This fluorescent dye has been used in sorting living cells, used in flow cytometry for the determination of DNA and used for visualization of chromatin distribution in living cells. Hoechst 33342 and DNA complex show light blue flourescent color with excitation light 355 nm and emission light 465 nm.

Immunofluorescence (IF) of Hoechst 33342 reagent

Immunofluorescent analysis staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. Hoechst 33342 was used to stain the cell nuclei (blue).
Hoechst 33342 Reagent – Cat# MBS826727

Other Notes

Small volumes of Hoechst 33342 reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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