Ponceau S Protein Reagent – Protein Marker

After the SDS-PAGE Gel has been transferred to a nitrocellulose or PVDF membrane, place the transferred membrane in a staining container.
Submerge the membrane in a proper amount of Ponceau S Protein Staining Solution that is enough to cover the whole membrane. Lightly agitate the staining container for 5 to 15 minutes at room temperature. Protein bands will be stained and start becoming visible in 5 to 15 minutes.
Wash away the excessive stain on the membrane with DI water until the protein bands are clear.
For the further membrane blocking step, remove the Ponceau S Staining Solution from the membrane by placing the membrane in 20 ml 0.1% NaOH for 1 to 2 minutes then rinse in DI water for 1 to 2 minutes.

The Ponceau S Protein Staining Solution is provided as a ready-to-use solution, there is no need to dilute before use.

Proteomic Products; Protein Marker; ECL; Protein Stain; TMB; Staining Reagent; Ponceau S; Protein Stain; Western blot membranes; SDS-PAGE; Gel Electrophoresis; Polyacrylamide Gel; Polyvinylidene fluoride (PVDF) membrane; Nitrocellulose membrane; Cellulose