Recombinant ALKBH8 protein gel 10% SDS-PAGE Coomassie staining MW: 77.9 kDa Purity: >48%

ALKBH8 Recombinant Protein

Name: ALKBH8 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS389250
Price: 735 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS389250

Short Description: Full-length ALKBH8 protein (accession number NP_001287939.1) was expressed in E Coli with an N-terminal 6xHis-tag. The molecular weight of ALKBH8 is 77.9 kDa.Background: ALKBH8 (AlkB Homolog 8), also called as ABH8 or TRMT9A, is a… More details

$735 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ALKBH8, Recombinant Protein

Full Product Name

Recombinant ALKBH8 protein

Product Gene Name

ALKBH8 recombinant protein

Product Synonym Names

AlkB Homolog 8; TRNA Methyltransferase; S-Adenosyl-L-Methionine-Dependent TRNA

Host

E Coli

Form/Format

Recombinant ALKBH8 protein is supplied in 25mM Tris-HCl pH8.0, 300mM NaCl, 10% glycerol, and 0.5mM TCEP.

Preparation and Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice

Molecular Weight

75,558 Da

OMIM

613306

3D Structure

ModBase 3D Structure for Q96BT7

Protein Species

Human

Tag

His-Tag

Notes

This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Dry Ice Shipment

Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for ALKBH8 recombinant protein

Recombinant Proteins; DNA Damage; Transcription

Other Notes

Small volumes of ALKBH8 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ALKBH8 purchase

MBS389250 is a recombinant protein targeting ALKBH8. Also known as Recombinant ALKBH8 protein. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for ALKBH8. It may not necessarily be applicable to this product.

NCBI GI #

666335573

NCBI GeneID

91801

NCBI Accession #

NP_001287939.1

UniProt Primary Accession #

Q96BT7

UniProt Secondary Accession #

Q8N989; B1Q2M0; B4DEF6

UniProt Related Accession #

Q96BT7

GenBank Nucleotide #

NM_001301010.1

NCBI Official Full Name

alkylated DNA repair protein alkB homolog 8 isoform 1

NCBI Official Synonym Full Names

alkB homolog 8, tRNA methyltransferase

NCBI Official Symbol

ALKBH8

NCBI Official Synonym Symbols

ABH8; TRM9; TRMT9; TRMT9A

UniProt Protein Name

Alkylated DNA repair protein alkB homolog 8

UniProt Synonym Protein Names

Probable alpha-ketoglutarate-dependent dioxygenase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8

Protein Family

Alkylated DNA repair protein

UniProt Gene Name

ALKBH8

UniProt Synonym Gene Names

ABH8

UniProt Entry Name

ALKB8_HUMAN

NCBI Protein Information

alkylated DNA repair protein alkB homolog 8

UniProt Comments for ALKBH8

ALKBH8: Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis. Belongs to the alkB family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.229; Methyltransferase; RNA processing

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: microtubule cytoskeleton; nucleoplasm; nucleus; cytosol

Molecular Function: protein binding; zinc ion binding; tRNA (uracil) methyltransferase activity; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; tRNA binding

Biological Process: tRNA wobble uridine modification; response to DNA damage stimulus; tRNA methylation

Research Articles on ALKBH8

1. findings suggest that the high expression of ALKBH8 is critical for the growth and progression of bladder cancer

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ALKBH8 recombinant protein

Reference Product	PubMed Publications
ALKBH1 recombinant protein	>4 publications with ALKBH8 and ALKBH1
ALKBH2 recombinant protein	>2 publications with ALKBH8 and ALKBH2
ALKBH7 recombinant protein	>1 publications with ALKBH8 and ALKBH7
ALKBH3 recombinant protein	>1 publications with ALKBH8 and ALKBH3

Pathways associated with ALKBH8 recombinant protein

Products by Pathway	Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
TRNA Modification In The Nucleus And Cytosol Pathway antibodies	TRNA Modification In The Nucleus And Cytosol Pathway Diagram
TRNA Processing Pathway antibodies	TRNA Processing Pathway Diagram

Diseases associated with ALKBH8 recombinant protein

Disease Name	Pubmed Publications
Carcinoma Antibodies	>1 publications with ALKBH8 and Carcinoma

Organs/Tissues associated with ALKBH8 recombinant protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1 publications with ALKBH8 and Blood