a2M Recombinant Protein – Cat# MBS2010484

Alpha-2-Macroglobulin (a2M) Recombinant Protein

Name: a2M Recombinant Protein; Alpha-2-Macroglobulin; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS2010484
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2010484

MBS2010484 is a recombinant protein targeting a2M. Also known as Recombinant Alpha-2-Macroglobulin (a2M). Host: E Coli. More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Alpha-2-Macroglobulin (a2M), Recombinant Protein

Full Product Name

Recombinant Alpha-2-Macroglobulin (a2M)

Product Gene Name

a2M recombinant protein

Sequence

The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF- TCI RVAVQLEASP DFLAAPEEKE QRSHCICMNQ RHTASWAVIP KSLGNVNFTV SAEALNSKEL CGNEVPVVPE QGKKDTIIKS LLVEPEGLEN EVTFNSLLCP MGAEVSELIA LKLPSDVVEE SARASVTVLG DILGSAMQNT QDLLKMPYGC GEQNMVLFAP NIYVLDYLNE TQQLTQEIKT KAIAYLNTGY QRQLNYKHRD GSYSTFGDKP GRNHANTWLT AFVLKSFA

Host

E Coli

Form/Format

Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Purity/Purification

> 95%

Preparation and Storage

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Matching Pairs

Unconjugated Antibody: a2M (MBS2005313)
Immunogen: a2M (MBS2010484)

Matching Pairs

APC-CY7 Conjugated Antibody: a2M (MBS2050538)
Immunogen: a2M (MBS2010484)

Molecular Weight

27.0kDa

Chromosome Location

Chromosome: 4; NC_005103.3 (221393233..221442945). Location: 4q42

3D Structure

ModBase 3D Structure for P06238

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Organism

Rattus norvegicus (Rat)

Expression System

Prokaryotic expression

Residues

Thr818~Ala1048 (Accession # P06238) with N-terminal His-Tag

Endotoxin Level

<1.0EU per 1ug (determined by the LAL method)

Reconstitution

Reconstitute in sterile PBS, pH7.2-pH7.4.

ISO Certification

Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Applications Tested/Suitable for a2M recombinant protein

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Other Notes

Small volumes of a2M recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for a2M. It may not necessarily be applicable to this product.

NCBI GI #

158138551

NCBI GeneID

24153

NCBI Accession #

NP_036620.2

UniProt Primary Accession #

P06238

UniProt Secondary Accession #

Q4FZY3

UniProt Related Accession #

P06238

GenBank Nucleotide #

NM_012488.2

NCBI Official Full Name

alpha-2-macroglobulin

NCBI Official Synonym Full Names

alpha-2-macroglobulin

NCBI Official Symbol

A2m

NCBI Official Synonym Symbols

Mam; A2m1; A2maa; A2MAC1

UniProt Protein Name

Alpha-2-macroglobulin

Protein Family

Alpha-2-macroglobulin

UniProt Gene Name

A2m

UniProt Synonym Gene Names

A2m1; Alpha-2-M

UniProt Entry Name

A2MG_RAT

NCBI Protein Information

alpha-2-macroglobulin; alpha-2-M; alpha-2-macroglobulin-P

NCBI Summary for a2M

plays a role in acute phase response; activated form induces a decrease in N-methyl-D-aspartate (NMDA) mediated calcium signaling [RGD, Feb 2006]

UniProt Comments for a2M

Function: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Subunit structure: Homotetramer; disulfide-linked.

Subcellular location: Secreted.

Tissue specificity: Highest constitutive expression in ovary. Low level in testis, uterus and non-acute phase liver. Protein found in plasma. Ref.5

Induction: By inflammatory stimulus in liver. The level of this protein increases during acute phase, then decreases again. Ref.1

Sequence similarities: Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. [View classification]

Research Articles on a2M

1. Peak serum levels of alpha(2)-macroglobulin and alpha(1)-acid glycoprotein injected at 0.05 mL/rat were significantly lower than those at 0.2 or 0.4 mL/rat. No significant differences were observed for peak serum levels at 0.2 and 0.4 mL/rat.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with a2M recombinant protein

Products by Pathway	Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies	Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Intrinsic Pathway antibodies	Intrinsic Pathway Diagram
Platelet Activation, Signaling And Aggregation Pathway antibodies	Platelet Activation, Signaling And Aggregation Pathway Diagram
Platelet Degranulation Pathway antibodies	Platelet Degranulation Pathway Diagram

Diseases associated with a2M recombinant protein

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>92 publications with a2M and Nervous System Diseases
Brain Diseases Antibodies	>87 publications with a2M and Brain Diseases
Mental Disorders Antibodies	>80 publications with a2M and Mental Disorders
Alzheimer Disease Antibodies	>78 publications with a2M and Alzheimer Disease
Neoplasms Antibodies	>32 publications with a2M and Neoplasms
Inflammation Antibodies	>29 publications with a2M and Inflammation
Necrosis Antibodies	>14 publications with a2M and Necrosis
Liver Diseases Antibodies	>14 publications with a2M and Liver Diseases
Lung Diseases Antibodies	>11 publications with a2M and Lung Diseases
Fibrosis Antibodies	>11 publications with a2M and Fibrosis

Organs/Tissues associated with a2M recombinant protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>37 publications with a2M and Liver
Brain Antibodies	>30 publications with a2M and Brain
Embryonic Tissue Antibodies	>6 publications with a2M and Embryonic Tissue
Placenta Antibodies	>5 publications with a2M and Placenta
Ovary Antibodies	>4 publications with a2M and Ovary
Spleen Antibodies	>3 publications with a2M and Spleen
Connective Tissue Antibodies	>2 publications with a2M and Connective Tissue
Colon Antibodies	>1 publications with a2M and Colon