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CD5 Biotinylated Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553785

MBS553785 is a recombinant protein targeting CD5. Also known as Biotinylated Human CD5 Protein Recombinant. Host: HEK293 cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.). More details

$725 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD5, Recombinant Protein
Full Product Name
Biotinylated Human CD5 Protein Recombinant
Product Gene Name
Host
HEK293 cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
Form/Format
Recombinant Human CD5 was lyophilized from 0.2 um filtered PBS solution pH7.4
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at-20 degree C.
Molecular Weight
54,578 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (60869930..60895324). Location: 11q13
OMIM
153340
3D Structure
ModBase 3D Structure for P06127
Source
DNA sequence encoding Human CD5 including a C-hFc-Avi tag was expressed in HEK293 Cells.The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM

Product Categories/Family for CD5 recombinant protein

Tris-Bis-Page of CD5 recombinant protein

Biotinylated Human CD5 Protein Tris-Bis PAGE
Biotinylated Human CD5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human CD5 Recombinant Protein – Cat# MBS553785

Testing Data of CD5 recombinant protein

Biotinylated Human CD5 Protein SEC-HPLC
The purity of Biotinylated Human CD5 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CD5 Recombinant Protein, Testing Data – Cat# MBS553785

ELISA (EIA) of CD5 recombinant protein

Biotinylated Human CD5 Protein ELISA Data
Immobilized Anti-CD5 Antibody, hFc Tag at 1?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human CD5, hFc Tag with the EC50 of 16.0ng/ml determined by ELISA.
Biotinylated Human CD5 Recombinant Protein, ELISA (EIA) – Cat# MBS553785

Other Notes

Small volumes of CD5 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
T-cell surface glycoprotein CD5
NCBI Official Synonym Full Names
CD5 molecule
NCBI Official Symbol
CD5
NCBI Official Synonym Symbols
T1; LEU1
UniProt Protein Name
T-cell surface glycoprotein CD5
UniProt Synonym Protein Names
Lymphocyte antigen T1/Leu-1
UniProt Gene Name
CD5
UniProt Synonym Gene Names
LEU1
UniProt Entry Name
CD5_HUMAN
NCBI Protein Information
T-cell surface glycoprotein CD5; CD5 antigen (p56-62); lymphocyte antigen T1/Leu-1

UniProt Comments for CD5

CD5: a surface glycoprotein expressed on T cells and B-1 B cells. Rapidly recruited to the immunological synapse, lowering the response of the T cell antigen receptor. CD5 interacts with CD72/LYB-2. Promotes B-cell survival through stimulation of autocrine IL-10 production. Contains 3 SRCR domains.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor activity; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; cell proliferation; T cell costimulation; cell recognition
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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