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CERT Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203712

CERT is a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this… More details

$220 / 0.02 mg +1 FREE 8GB USB
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CERT, Recombinant Protein
Full Product Name
CERT, 347-598aa, Human, His tag, E Coli
Product Gene Name
Product Synonym Names
Collagen type IV alpha 3 binding protein; COL4A3BP; CERTL; GPBP; STARD11; Collagen; type IV; alpha 3 binding protein Alpha 3 type IV collagen binding protein; Ceramide transporter; CERT; Cert protein; Collagen type IV alpha 3 (Goodpasture antigen) binding protein; Collagen type IV alpha 3 binding protein; FLJ20597; Goodpasture antigen binding protein; Lipid transfer protein CERTL; Procollagen type IV alpha 3 (Goodpasture antigen) binding protein; StAR related lipid transfer protein 11; StARD 11; START domain containing 11; START domain containing protein 11
Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMLH WPTSLPSGDA FSSVGTHRFV QKVEEMVQNH MTYSLQDVGG DANWQLVVEE GEMKVYRREV EENGIVLDPL KATHAVKGVT GHEVCNYFWN VDVRNDWETT IENFHVVETL ADNAIIIYQT HKRVWPASQR DVLYLSVIRK IPALTENDPE TWIVCNFSVD HDSAPLNNRC VRAKINVAMI CQTLVSPPEG NQEISRDNIL CKITYVANVN PGGWAPASVL RAVAKREYPK FLKRFTSYVQ EKTAGKPILF
Host
E Coli
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 0.1M NaCl
Purity/Purification
> 95% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
33.1 kDa (290aa) confirmed by MALDI-TOF
OMIM
AF136450 mRNA
3D Structure
ModBase 3D Structure for Q9Y5P4
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for CERT recombinant protein

Applications Tested/Suitable for CERT recombinant protein
SDS-PAGE

Product References and Citations for CERT recombinant protein

Turner N., et al. (1992) J Clin Invest 89 (2):592-601.

Other Notes

Small volumes of CERT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CERT purchase

MBS203712 is a recombinant protein targeting CERT. Also known as CERT, 347-598aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CERT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
collagen type IV alpha-3-binding protein isoform 2
NCBI Official Synonym Full Names
collagen type IV alpha 3 binding protein
NCBI Official Symbol
COL4A3BP
NCBI Official Synonym Symbols
CERT; GPBP; CERTL; MRD34; STARD11
UniProt Protein Name
Collagen type IV alpha-3-binding protein
UniProt Synonym Protein Names
Ceramide transfer protein
UniProt Gene Name
COL4A3BP
UniProt Synonym Gene Names
CERT; STARD11; hCERT; GPBP; StARD11
NCBI Protein Information
collagen type IV alpha-3-binding protein

NCBI Summary for CERT

This gene encodes a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for CERT

Shelters ceramides and diacylglycerol lipids inside its START domain and mediates the intracellular trafficking of ceramides and diacylglycerol lipids in a non-vesicular manner.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CERT recombinant protein

 Reference Product  PubMed Publications
 COL4A3 recombinant protein  >1 publications with CERT and COL4A3
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