COMT Recombinant Protein

Name: COMT Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203262
Price: 300 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203262

Catechol-O-methyltrasferase (COMT) is an enzyme that catalyses the transfer of the methyl group of S-adenosylmethionine (SAM) into catechol substrates such as levodopa, dopamine, epinephrine, and norepinephirine in the presence of Mg2+. COMT appears to be… More details

$300 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

COMT, Recombinant Protein

Full Product Name

COMT, 51-271aa, Human, E Coli

Product Gene Name

COMT recombinant protein

Product Synonym Names

Catechol-O-methyltransferase; Catechol-O-methyltransferase; Catechol-O-methyltransferase; COMT

Sequence

MGDTKEQRIL NHVLQHAEPG NAQSVLEAID TYCEQKEWAM NVGDKKGKIV DAVIQEHQPS VLLELGAYCG YSAVRMARLL SPGARLITIE INPDCAAITQ RMVDFAGVKD KVTLVVGASQ DIIPQLKKKY DVDTLDMVFL DHWKDRYLPD TLLLEECGLL RKGTVLLADN VICPGAPDFL AHVRGSSCFE CTHYQSFLEY REVVDGLEKA IYKGPGSEAG P

Host

E Coli

Form/Format

Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1 mM MgCl2, 10% glycerol

Purity/Purification

> 95% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

24.4 kDa (221 aa), confirmed by MALDI-TOF.

OMIM

M65212 mRNA

3D Structure

ModBase 3D Structure for P21964

Antigen Species

Human

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Product Categories/Family for COMT recombinant protein

Enzymes & Proteases

Applications Tested/Suitable for COMT recombinant protein

SDS-PAGE

Product References and Citations for COMT recombinant protein

Cotton NJ., et al. (2004) J Biol Chem. 279 (22):23710-8. ; Dawling S., et al. (2001) Cancer Res. 61 (18):6716-22.;

Other Notes

Small volumes of COMT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for COMT purchase

MBS203262 is a recombinant protein targeting COMT. Also known as COMT, 51-271aa, Human, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for COMT. It may not necessarily be applicable to this product.

NCBI GI #

4502969

NCBI GeneID

1312

NCBI Accession #

NP_000745

UniProt Primary Accession #

P21964

UniProt Secondary Accession #

Q6IB07; Q6ICE6; Q9BWC7; A8MPV9

UniProt Related Accession #

P21964

GenBank Nucleotide #

NM_000754.3

NCBI Official Full Name

catechol O-methyltransferase isoform MB-COMT

NCBI Official Synonym Full Names

catechol-O-methyltransferase

NCBI Official Symbol

COMT

NCBI Official Synonym Symbols

HEL-S-98n

UniProt Protein Name

Catechol O-methyltransferase

Protein Family

Catechol O-methyltransferase

UniProt Gene Name

COMT

NCBI Protein Information

catechol O-methyltransferase

NCBI Summary for COMT

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008]

UniProt Comments for COMT

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.

Research Articles on COMT

1. It can be concluded that the COMT rs4680:G>A polymorphism is not associated with opioid demand in patients after elective hip replacement.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with COMT recombinant protein

Reference Product	PubMed Publications
CYP1B1 recombinant protein	>134 publications with COMT and CYP1B1
CYP1A1 recombinant protein	>130 publications with COMT and CYP1A1
MAOA recombinant protein	>124 publications with COMT and MAOA
DBH recombinant protein	>93 publications with COMT and DBH
MAOB recombinant protein	>42 publications with COMT and MAOB
DDC recombinant protein	>40 publications with COMT and DDC
UGT2B7 recombinant protein	>29 publications with COMT and UGT2B7
PNMT recombinant protein	>29 publications with COMT and PNMT
UGT1A1 recombinant protein	>22 publications with COMT and UGT1A1
UGT1A9 recombinant protein	>6 publications with COMT and UGT1A9

Pathways associated with COMT recombinant protein

Products by Pathway	Pathway Diagram
Biogenic Amine Synthesis Pathway antibodies	Biogenic Amine Synthesis Pathway Diagram
Biological Oxidations Pathway antibodies	Biological Oxidations Pathway Diagram
Dopamine Clearance From The Synaptic Cleft Pathway antibodies	Dopamine Clearance From The Synaptic Cleft Pathway Diagram
Dopamine Metabolism Pathway antibodies	Dopamine Metabolism Pathway Diagram
Dopaminergic Synapse Pathway antibodies	Dopaminergic Synapse Pathway Diagram
Dopaminergic Synapse Pathway antibodies	Dopaminergic Synapse Pathway Diagram
Enzymatic Degradation Of Dopamine By Monoamine Oxidase Pathway antibodies	Enzymatic Degradation Of Dopamine By Monoamine Oxidase Pathway Diagram
Enzymatic Degradation Of Dopamine By COMT Pathway antibodies	Enzymatic Degradation Of Dopamine By COMT Pathway Diagram
Estrogen Metabolism Pathway antibodies	Estrogen Metabolism Pathway Diagram
L-dopa Degradation Pathway antibodies	L-dopa Degradation Pathway Diagram

Diseases associated with COMT recombinant protein

Disease Name	Pubmed Publications
Mental Disorders Antibodies	>1278 publications with COMT and Mental Disorders
Nervous System Diseases Antibodies	>720 publications with COMT and Nervous System Diseases
Schizophrenia Antibodies	>620 publications with COMT and Schizophrenia
Schizophrenia Spectrum and Other Psychotic Disorders Antibodies	>493 publications with COMT and Schizophrenia Spectrum and Other Psychotic Disorders
Movement Disorders Antibodies	>473 publications with COMT and Movement Disorders
Pain Antibodies	>252 publications with COMT and Pain
Cognition Disorders Antibodies	>212 publications with COMT and Cognition Disorders
Cardiovascular Diseases Antibodies	>189 publications with COMT and Cardiovascular Diseases
Breast Neoplasms Antibodies	>175 publications with COMT and Breast Neoplasms
Bipolar Disorder Antibodies	>151 publications with COMT and Bipolar Disorder

Organs/Tissues associated with COMT recombinant protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>1377 publications with COMT and Brain
Blood Antibodies	>782 publications with COMT and Blood
Liver Antibodies	>293 publications with COMT and Liver
Nerve Antibodies	>165 publications with COMT and Nerve
Heart Antibodies	>150 publications with COMT and Heart
Muscle Antibodies	>146 publications with COMT and Muscle
Vascular Antibodies	>142 publications with COMT and Vascular
Kidney Antibodies	>121 publications with COMT and Kidney
Lung Antibodies	>61 publications with COMT and Lung
Intestine Antibodies	>49 publications with COMT and Intestine