• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CRKL Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515385

Recombinant full length human CRKL was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.Scientific Background: CRKL or v-crk sarcoma virus CT10 oncogene homolog (avian)-like is a protein kinase containing SH2 and SH3 (src homology)… More details

$300 / 0.02 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CRKL, Recombinant Protein
Full Product Name
CRKL recombinant protein
Product Gene Name
Product Synonym Gene Name
crk-like protein
Product Synonym Names
crk-like protein
Host
Sf9 insect cells
Form/Format
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
~39kDa
Chromosome Location
Chromosome: 22; NC_000022.10 (21271714..21308037). Location: 22q11.21
OMIM
602007
3D Structure
ModBase 3D Structure for P46109
Type
Recombinant Fusion Protein
Species
Human
Tag Information
His tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full length human CRKL was expressed by baculovirus in Sf9 insect cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Applications Tested/Suitable for CRKL recombinant protein
Western Blot (WB)

Product References and Citations for CRKL recombinant protein

1. Ten Hoeve.J et.al: Isolation and chromosomal localization of CRKL, a human crk-like gene. Oncogene 8: 2469-2474, 1993.
2. Senechal, K. et.al: The CRKL adaptor protein transforms fibroblasts and functions in transformation by the BCR-ABL oncogene. J. Biol. Chem. 38: 23255-23261, 1996.

Other Notes

Small volumes of CRKL recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CRKL purchase

MBS515385 is a recombinant protein targeting CRKL. Host: Sf9 insect cells.

Additional Details

NCBI/Uniprot data below describe general gene information for CRKL. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens v-crk avian sarcoma virus CT10 oncogene homolog-like (CRKL), mRNA
NCBI Official Synonym Full Names
v-crk avian sarcoma virus CT10 oncogene homolog-like
NCBI Official Symbol
CRKL
UniProt Protein Name
Crk-like protein
Protein Family
UniProt Gene Name
CRKL
UniProt Entry Name
CRKL_HUMAN
NCBI Protein Information
crk-like protein

NCBI Summary for CRKL

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009]
Show more

UniProt Comments for CRKL

Function: May mediate the transduction of intracellular signals.

Subunit structure: Interacts with INPP5D/SHIP1. Interacts with DOCK2 and EPOR. Interacts with phosphorylated CBLB and IRS4. Ref.6 Ref.7 Ref.8 Ref.9

Sequence similarities: Belongs to the CRK family.Contains 1 SH2 domain.Contains 2 SH3 domains.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000