Human CXCL4 Recombinant Protein – Cat# MBS9719735

CXCL4 Human Recombinant Protein

Name: CXCL4 Recombinant Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS9719735
Price: 140 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9719735

C-X-C motif chemokine 4 (CXCL4) also named platelet factor 4 (PF4), which is a chemokine of the intercrine alpha family. CXCL4 is a 8kDa protein containing 70 amino acid residues. CXCL4 is produced by the activated platelets which plays an important role in… More details

$140 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CXCL4, Recombinant Protein

Full Product Name

Human CXCL4 Protein, His tag (Animal-Free)

Product Gene Name

CXCL4 recombinant protein

Product Synonym Names

CXCL4; CXCL-4; CXCL 4; Platelet Factor-4; PF-4; Oncostatin A; Ironplact

Sequence

Glu32-Ser1 01

Host

E coli

Form/Format

Lyophilized from sterile PBS, pH 7.4.

Purity/Purification

>98 % as determined by SDS-PAGE

Preparation and Storage

Lyophilized protein product should be stored desiccated below -18 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Shipping: Gel pack with blue ice.

Molecular Weight

10,845 Da

Sequence Positions

70aa

Chromosome Location

Chromosome: 4; NC_000004.11 (74846542..74847841, complement). Location: 4q12-q21

OMIM

173460

3D Structure

ModBase 3D Structure for P02776

Species

Human

Tag

His tag

Endotoxin

<0.1 EU per ug of the protein as determined by the LAL method.

Activity

Measure by its ability to inhibit human FGF-2-induce proliferation in HUVEC cells.The ED?? for this effect is <5ug/mL.

Protein Construction

The recombinant Human CXCL4 consists of 70 amino acids and predicts a molecular mass of 8.58kDa.
Amino acid sequence derived from Human CXCL4 (Glu32-Ser101)(P02776) was expressed with 6×His tag at the N-terminus.

Usage Notes

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.

Product Categories/Family for CXCL4 recombinant protein

Cytokines and proteins

Other Notes

Small volumes of CXCL4 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CXCL4 purchase

MBS9719735 is a recombinant protein targeting CXCL4. Also known as Human CXCL4 Protein, His tag (Animal-Free). Host: E coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CXCL4. It may not necessarily be applicable to this product.

NCBI GI #

4505733

NCBI GeneID

5196

NCBI Accession #

NP_002610.1

UniProt Primary Accession #

P02776

UniProt Secondary Accession #

Q53X61; Q9UC64; Q9UC65

UniProt Related Accession #

P02776

GenBank Nucleotide #

NM_002619.3

NCBI Official Full Name

platelet factor 4

NCBI Official Synonym Full Names

platelet factor 4

NCBI Official Symbol

PF4

NCBI Official Synonym Symbols

PF-4; CXCL4; SCYB4

UniProt Protein Name

Platelet factor 4

UniProt Synonym Protein Names

C-X-C motif chemokine 4; Iroplact; Oncostatin-A

UniProt Gene Name

PF4

UniProt Synonym Gene Names

CXCL4; SCYB4; PF-4

UniProt Entry Name

PLF4_HUMAN

NCBI Protein Information

platelet factor 4; iroplact; oncostatin-A; C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4

NCBI Summary for CXCL4

This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. [provided by RefSeq, Mar 2012]

UniProt Comments for CXCL4

PF4: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Apoptosis; Chemokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q12-q21

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; CXCR3 chemokine receptor binding; chemokine activity

Biological Process: negative regulation of cytolysis; platelet activation; cytokine and chemokine mediated signaling pathway; leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; positive regulation of cAMP metabolic process; positive regulation of tumor necrosis factor production; negative regulation of megakaryocyte differentiation; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; platelet degranulation; negative regulation of MHC class II biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response; positive regulation of macrophage differentiation; blood coagulation

Research Articles on CXCL4

1. Report ability of enoxaparin-derived oligosaccharides to induce platelet activation and exposure of platelet-factor 4 epitopes recognized by antibodies developed in heparin induced thrombocytopenia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CXCL4 recombinant protein

Reference Product	PubMed Publications
FGF2 recombinant protein	>16 publications with CXCL4 and FGF2
CXCR3 recombinant protein	>14 publications with CXCL4 and CXCR3
PROC recombinant protein	>11 publications with CXCL4 and PROC
CXCL11 recombinant protein	>5 publications with CXCL4 and CXCL11

Pathways associated with CXCL4 recombinant protein

Products by Pathway	Pathway Diagram
CXCR3-mediated Signaling Events Pathway antibodies	CXCR3-mediated Signaling Events Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Chemokine Receptors Bind Chemokines Pathway antibodies	Chemokine Receptors Bind Chemokines Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Common Pathway antibodies	Common Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies	Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram

Diseases associated with CXCL4 recombinant protein

Disease Name	Pubmed Publications
Thrombocytopenia Antibodies	>423 publications with CXCL4 and Thrombocytopenia
Cardiovascular Diseases Antibodies	>352 publications with CXCL4 and Cardiovascular Diseases
Thrombosis Antibodies	>286 publications with CXCL4 and Thrombosis
Heart Diseases Antibodies	>117 publications with CXCL4 and Heart Diseases
Neoplasms Antibodies	>96 publications with CXCL4 and Neoplasms
Inflammation Antibodies	>70 publications with CXCL4 and Inflammation
Nervous System Diseases Antibodies	>66 publications with CXCL4 and Nervous System Diseases
Autoimmune Diseases Antibodies	>58 publications with CXCL4 and Autoimmune Diseases
Kidney Diseases Antibodies	>54 publications with CXCL4 and Kidney Diseases
Cerebral Infarction Antibodies	>35 publications with CXCL4 and Cerebral Infarction

Organs/Tissues associated with CXCL4 recombinant protein

Organ/Tissue Name	Pubmed Publications
Heart Antibodies	>92 publications with CXCL4 and Heart
Bone Antibodies	>70 publications with CXCL4 and Bone
Bone Marrow Antibodies	>61 publications with CXCL4 and Bone Marrow
Skin Antibodies	>32 publications with CXCL4 and Skin
Lung Antibodies	>31 publications with CXCL4 and Lung
Embryonic Tissue Antibodies	>18 publications with CXCL4 and Embryonic Tissue
Intestine Antibodies	>6 publications with CXCL4 and Intestine
Placenta Antibodies	>1 publications with CXCL4 and Placenta