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DFFA Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203369

DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA, also known as DNA fragmentation factor subunit alpha, is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
DFFA, Recombinant Protein
Full Product Name
DFFA, 1-331aa, Human, His tag, E Coli
Product Gene Name
DFFA recombinant protein
Product Synonym Names
DNA fragmentation factor subunit alpha.; FF-45; DFF1; ICAD; DNA fragmentation factor subunit alpha; A330085O09Rik; ICAD L; ICAD S; DFF 1; Caspase activated deoxyribonuclease inhibitor short form; DFF 45; DFF alpha; DFF35; DFF45; DFFA; Caspase-activated deoxyribonuclease inhibitor short form; Dffa DNA fragmentation factor; alpha subunit; DNA fragmentation factor 45 kDa subunit; DNA Fragmentation Factor Alpha Subunit; DNA fragmentation factor subunit alpha; DNA fragmentation factor; 45 kD; alpha subunit; DNA fragmentation factor; 45kDa; alpha polypeptide; DNA fragmentation factor; 45kDa; alpha polypeptide (DFFA); transcript variant 1; RP23 121D17.3; DNA fragmentation factor; alpha subunit; DNAation factor 45 kDa subunit; H13; Inhibitor of CAD; Inhibitor of Caspase Activated DNase; MGC143066; OTTHuMP00000001903; OTTHuMP00000001904
Sequence
MGSSHHHHHH SSGLVPRGSH MEVTGDAGVP ESGEIRTLKP CLLRRNYSRE QHGVAASCLE DLRSKACDIL AIDKSLTPVT LVLAEDGTIV DDDDYFLCLP SNTKFVALAS NEKWAYNNSD GGTAWISQES FDVDETDSGA GLKWKNVARQ LKEDLSSIIL LSEEDLQMLV DAPCSDLAQE LRQSCATVQR LQHTLQQVLD QREEVRQSKQ LLQLYLQALE KEGSLLSKQE ESKAAFGEEV DAVDTGISRE TSSDVALASH ILTALREKQA PELSLSSQDL ELVTKEDPKA LAVALNWDIK KTETVQEACE WELALRLQQT QSLHSLRSIS ASKASPPGDL QNPKRARQDP T
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 1mM DTT.
Purity/Purification
> 90% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
38.7 kDa (351aa), confirmed by MALDI-TOF.
OMIM
U91985 mRNA
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for DFFA recombinant protein

Apoptosis
Applications Tested/Suitable for DFFA recombinant protein
SDS-PAGE

SDS-Page of DFFA recombinant protein

DFFA Recombinant Protein – Cat# MBS203369

Product References and Citations for DFFA recombinant protein

McCarty JS., et al. (1999) Biochem Biophys Res Commun. 264 (1):176-80. ; Gu J., et al. (1999) J Biol Chem. 274 (30):20759-62.;

Other Notes

Small volumes of DFFA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DFFA purchase

MBS203369 is a recombinant protein targeting DFFA. Also known as DFFA, 1-331aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for DFFA. It may not necessarily be applicable to this product.

NCBI GI #
14043461
NCBI GeneID
1676
NCBI Accession #
AAH07721
UniProt Secondary Accession #
Q5T6G5; Q5T6G6; Q96I97; Q9Y6C6
UniProt Related Accession #
O00273
NCBI Official Full Name
DNA fragmentation factor, 45kDa, alpha polypeptide
NCBI Official Synonym Full Names
DNA fragmentation factor subunit alpha
NCBI Official Symbol
DFFA
NCBI Official Synonym Symbols
DFF1; ICAD; DFF-45
UniProt Protein Name
DNA fragmentation factor subunit alpha
UniProt Synonym Protein Names
DNA fragmentation factor 45 kDa subunit; DFF-45; Inhibitor of CAD; ICAD
Protein Family
DNA fragmentation factor
UniProt Gene Name
DFFA
UniProt Synonym Gene Names
DFF1; DFF45; DFF-45; ICAD
NCBI Protein Information
DNA fragmentation factor subunit alpha

NCBI Summary for DFFA

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for DFFA

Inhibitor of the caspase-activated DNase (DFF40).

Research Articles on DFFA

1. evaluate the relative expression levels of miR-196a2 and three of its selected apoptosis-related targets; ANXA1, DFFA and PDCD4 in a sample of GI cancer patients

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DFFA recombinant protein

 Reference Product  PubMed Publications
 CIDEC recombinant protein  >18 publications with DFFA and CIDEC
 CIDEB recombinant protein  >11 publications with DFFA and CIDEB
 CASP3 recombinant protein  >6 publications with DFFA and CASP3
 DFFB recombinant protein  >6 publications with DFFA and DFFB
 CASP8 recombinant protein  >4 publications with DFFA and CASP8
 CASP9 recombinant protein  >1 publications with DFFA and CASP9

Pathways associated with DFFA recombinant protein

 Products by Pathway  Pathway Diagram
 Activation Of DNA Fragmentation Factor Pathway antibodies  Activation Of DNA Fragmentation Factor Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 Apoptosis Induced DNA Fragmentation Pathway antibodies  Apoptosis Induced DNA Fragmentation Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Fas Ligand (FasL) Pathway And Stress Induction Of Heat Shock Proteins (HSP) Regulation antibodies  Fas Ligand (FasL) Pathway And Stress Induction Of Heat Shock Proteins (HSP) Regulation Diagram

Diseases associated with DFFA recombinant protein

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >11 publications with DFFA and Necrosis
 Inflammation Antibodies  >9 publications with DFFA and Inflammation
 Disease Models, Animal Antibodies  >8 publications with DFFA and Disease Models, Animal
 Fatty Liver Antibodies  >7 publications with DFFA and Fatty Liver
 Cardiovascular Diseases Antibodies  >7 publications with DFFA and Cardiovascular Diseases
 Liver Diseases Antibodies  >6 publications with DFFA and Liver Diseases
 Fibrosis Antibodies  >4 publications with DFFA and Fibrosis
 Nervous System Diseases Antibodies  >3 publications with DFFA and Nervous System Diseases
 Heart Diseases Antibodies  >3 publications with DFFA and Heart Diseases
 Weight Loss Antibodies  >2 publications with DFFA and Weight Loss

Organs/Tissues associated with DFFA recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >19 publications with DFFA and Connective Tissue
 Liver Antibodies  >14 publications with DFFA and Liver
 Blood Antibodies  >11 publications with DFFA and Blood
 Muscle Antibodies  >6 publications with DFFA and Muscle
 Heart Antibodies  >5 publications with DFFA and Heart
 Bone Antibodies  >4 publications with DFFA and Bone
 Vascular Antibodies  >4 publications with DFFA and Vascular
 Brain Antibodies  >4 publications with DFFA and Brain
 Kidney Antibodies  >3 publications with DFFA and Kidney
 Bone Marrow Antibodies  >2 publications with DFFA and Bone Marrow
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