DOT1L Recombinant Protein

Background: Histone-lysine N-methyltransferase, H3 lysine-79 specific, also known as Histone H3-K79 methyltransferase, DOT1-like protein, Lysine N-methyltransferase 4 and DOT1L, is a nucleus protein which belongs to theDOT1 family. In contrast to other lysine histone methyltransferase, DOT1L does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones. DOT1L is an histone methyltransferase. It methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. DOT1L binds to DNA. Methylation of lysine 79 on histone H3 (H3K79) is mediated by DOT1L. It is involved in the regulation of telomeric silencing, development, cell cycle checkpoint and transcription. Mass spectrometry of the DOT1L-containing complex revealed that AF9, ENL and NPM1 were shown to be major DOT1L-interacting proteins. DOT1L might control AF9- and ENL-mediated transcription, regulate RNA processing, and function as a histone chaperone in a NPM1-dependent manner.

Nagase T. et al., 2001, DNA Res. 8:85-95. Feng Q.et al., 2002, Curr. Biol. 12:1052-8. Dephoure N. et al., 2008, Proc. Natl. Acad. Sci. USA. 105:10762-7. Gauci S. et al., 2009, Anal. Chem. 81:4493-501. Park,G. et al., 2010, Protein J. 29 (3):213-23.

Small volumes of DOT1L recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS8120849 is a recombinant protein targeting DOT1L. Also known as DOT1L Protein, Human, Recombinant. Host: E Coli.