GART Human Recombinant Protein

The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

GART: a highly conserved trifunctional enzyme. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. Inhibition of the de novo purine synthesis by folate analogs blocks the p53-dependent G1 checkpoint in human carcinoma cell lines. Two splice variant isoforms have been described.

Protein type: Methyltransferase; Ligase; EC 6.3.3.1; Nucleotide Metabolism - purine; EC 2.1.2.2; EC 6.3.4.13; Cofactor and Vitamin Metabolism - one carbon pool by folate; Mitochondrial; Enzyme, cellular metabolism

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: cytosol

Molecular Function: methyltransferase activity; metal ion binding; phosphoribosylamine-glycine ligase activity; phosphoribosylformylglycinamidine cyclo-ligase activity; phosphoribosylglycinamide formyltransferase activity; ATP binding

Biological Process: methylation; response to organic substance; purine ribonucleoside monophosphate biosynthetic process; glycine metabolic process; tetrahydrofolate biosynthetic process; response to inorganic substance; purine base biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; cerebellum development; cerebral cortex development; purine base metabolic process; 'de novo' IMP biosynthetic process