KMT2B Recombinant Protein

Short Description: Recombinant KMT2B (MLL4) complex contains amino acids 2551-2715 of the human MLL4 protein (accession number NP_055542.1) with an N-terminal GST-Tag and MW = 47.6 kDa; full-length human WDR5 (accession number NM_017588), with N-terminal 6xHis-tag and MW = 35 kDa; full-length human ASH2L (accession number NM_001105214), N-terminal 6xHis-tag and MW = 61 kDa; full-length human RbBP5 (accession number NM_005057), N-terminal 6xHis-tag and MW = 60 kDa, and full-length human DPY30 (accession number NM_032574), N-terminal 6xHis-tag and MW =12 kDa, all individually expressed in E Coli. The recombinant protein is >85% pure by SDS-PAGE. Recombinant KMT2B (MLL4) complex is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Background: KMT2B (MLL4)(Myeloid/lymphoid or mixed-lineage leukemia 4) is a Trithorax-group protein that function collectively to promote gene expression. KMT2B (MLL4) is a histone methyltransferase that methylates the Lysine 4 position of histone H3. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. H3K4 methylation represents a specific epigenetic tag for transcriptional activation. MLL4 plays a central role in b-globin locus transcription regulation by being recruited by Nfe2. KMT2B (MLL4) is also required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that precedes resumption of meiosis, oocyte survival and normal zygotic genome activation. MLL4 may also be involved in human cancer as its expression has been shown to be upregulated in solid tumor cell lines.

Small volumes of KMT2B recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS388209 is a recombinant protein targeting KMT2B. Also known as Recombinant KMT2B (MLL4) complex. Host: E Coli.