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MEC (CCL28) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553304

The mucosa-associated epithelial chemokine CCL28 (mucosa-associated epithelial chemokine) is a common mucosal chemokine which is constitutively expressed by epithelial cells in most mucosal sites. Both CCL28 and CCL25 have recently been shown to be essential… More details

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Product Overview

Online Datasheet in PDF
Product Name
MEC (CCL28), Recombinant Protein
Full Product Name
Human MEC (CCL28) Recombinant
Product Gene Name
CCL28 recombinant protein
Product Synonym Gene Name
MEC
Product Synonym Names
Human MECK Recombinant; mec; ccl28; SCYA28
Sequence
AAVILHVKRR RICVSPHNHT VKQWMKVQAA KKNGKGNVCH RKKHHGKRNS NRAHQGKHET YGHKTPY
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Recombinant CCL-28 was lyophilized from PBS pH 7.4.
Purity/Purification
>97%, as determined by SDS-PAGE and HPLC
Preparation and Storage
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Molecular Weight
14,280 Da
OMIM
605240
3D Structure
ModBase 3D Structure for Q9NRJ3
Domain
IL8
Host Note
Optimized DNA sequence encoding Human CCL28 mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Native human MEC/CCL28 has a calculated molecular mass of approximately 12 kDa.

Recombinant CCL28 is a disulfide-linked homodimeric protein consisting of 109 amino acid residue subunits, and migrates as an approximately 12 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

Product Categories/Family for CCL28 recombinant protein

Cytokines

Other Notes

Small volumes of CCL28 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCL28 purchase

MBS553304 is a recombinant protein targeting CCL28. Also known as Human MEC (CCL28) Recombinant. Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for CCL28. It may not necessarily be applicable to this product.

NCBI GI #
22538811
NCBI GeneID
56477
NCBI Accession #
NP_683513.1
UniProt Primary Accession #
Q9NRJ3
GenBank Nucleotide #
NM_148672.2
NCBI Official Full Name
C-C motif chemokine 28
UniProt Protein Name
C-C motif chemokine 28
UniProt Synonym Protein Names
Mucosae-associated epithelial chemokine; MEC; Protein CCK1; Small-inducible cytokine A28
Protein Family
C-C motif chemokine
UniProt Gene Name
CCL28
UniProt Synonym Gene Names
SCYA28; MEC
UniProt Entry Name
CCL28_HUMAN

NCBI Summary for CCL28

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for resting CD4 or CD8 T cells and eosinophils. The product of this gene binds to chemokine receptors CCR3 and CCR10. This chemokine may play a role in the physiology of extracutaneous epithelial tissues, including diverse mucosal organs. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
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UniProt Comments for CCL28

CCL28: Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Chemokine; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 5p12

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity

Biological Process: elevation of cytosolic calcium ion concentration; immune response; chemotaxis; positive regulation of cell-matrix adhesion; response to nutrient
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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