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opa gene Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS505038

the Neisseria meningitidis serogroup C gene for the opacity protein has been expressed as a recombinant antigen fused to a his-tag in its N-terminus. It contains aa 30 to 251 of the complete 256 aa-protein. More details

$1195 / 1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
opa gene, Recombinant Protein
Full Product Name
opa gene recombinant
Product Synonym Names
RECOMBINANT ANTIGEN opa from Neisseria meningitidis serogroup C; opacity-associated adhesin proteins
Host
E Coli
Form/Format
Dry powder (lyophilized)
Purity/Purification
0.98
Concentration
3.30 mg/ml (lot specific)
Preparation and Storage
Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.
Recombinant Antigen
Neisseria meningitidis serogroup C opacity protein and related surface antigen (Virji et al., 1993).
Organism
Neisseria meningitidis serotype C
Tag
HIS
Molecular Weight Information
Determined by SDS-PAGE, the protein band runs between molecular markers of 25,000 and 35,000 Da, while relative molecular mass calculated from amino acid sequence is 32,272.5 Da.
Observation Notes
Proteins should be maintained frozen at high concentrations. The dilution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25 degree C without shaking to avoid aggregation. Prior making test dilutions and after defrost the protein, is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface.
Buffer
20 mM phosphate buffer pH 8, 10 mM NaCl, 0.1% polyoxyethylene (10) tridecyl ether, 250 mM Imidazol, 8 M urea
Batch Composition
Recombinant antigen with a his-tag in its N-terminus
Storage Buffer
20 mM phosphate buffer pH 8, 10 mM NaCl, 0.1% polyoxyethylene (10) tridecyl ether, 250 mM Imidazol, 8 M urea
Reconstitution
For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.

Product Categories/Family for opa gene recombinant protein

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for opa gene recombinant protein
Western Blot (WB)

Product References and Citations for opa gene recombinant protein

Virji, M., K. Makepeace, D. J. Ferguson, M. Achtman, and E. R. Moxon. Meningococcal Opa and Opc proteins: their role in colonization and invasion of humanepithelial and endothelial cells. 1993, Mol. Microbiol. 10:499- 510.
Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.

Other Notes

Small volumes of opa gene recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for opa gene purchase

MBS505038 is a recombinant protein targeting opa gene. Also known as opa gene recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for opa gene. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
opacity outermembrane protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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