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p70S6Kb Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515295

Recombinant full-length human p70S6Kb was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: p70S6Kb is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases and is activated by mitogenic… More details

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Product Overview

Online Datasheet in PDF
Product Name
p70S6Kb, Recombinant Protein
Full Product Name
p70S6Kb, Unactive recombinant protein
Product Synonym Gene Name
RPS6KB2; STK14B; KLS; P70-beta; P70-beta-1; P70-beta-2; S6K2; p70(S6K)-beta; S6K-beta2; SRK
Product Synonym Names
RPS6KB2; STK14B; KLS; P70-beta; P70-beta-1; P70-beta-2; S6K2; p70(S6K)-beta; S6K-beta2; SRK
Host
Sf9 insect cells
Form/Format
50mM Tris-HCl, pH 7.5, 50mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
~85 kDa
Chromosome Location
Chromosome: 11; NC_000011.9 (67195935..67202879). Location: 11q13.2
OMIM
608939
3D Structure
ModBase 3D Structure for Q9UBS0
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full-length human p70S6Kb was expressed by baculovirus in Sf9 insect cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Applications Tested/Suitable for p70S6Kb recombinant protein
Kinase Assay, Western Blot (WB)

Product References and Citations for p70S6Kb recombinant protein

1. Gout, I. et al: Molecular cloning and characterization of a novel p70 S6 kinase, p70 S6 kinase beta containing a proline-rich region. J. Biol. Chem. 273: 30061-30064, 1998.
2. Saitoh, M. et al: Cloning and characterization of p70(S6K-beta) defines a novel family of p70 S6 kinases. Biochem. Biophys. Res. Commun. 253: 470-476, 1998.

Other Notes

Small volumes of p70S6Kb recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for p70S6Kb purchase

MBS515295 is a recombinant protein targeting p70S6Kb. Also known as p70S6Kb, Unactive recombinant protein. Host: Sf9 insect cells.

Additional Details

NCBI/Uniprot data below describe general gene information for p70S6Kb. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens ribosomal protein S6 kinase, 70kDa, polypeptide 2, mRNA
NCBI Official Synonym Full Names
ribosomal protein S6 kinase, 70kDa, polypeptide 2
NCBI Official Symbol
RPS6KB2
NCBI Official Synonym Symbols
KLS; SRK; S6K2; STK14B; p70S6Kb; P70-beta; S6K-beta2; P70-beta-1; P70-beta-2; p70(S6K)-beta
UniProt Protein Name
Ribosomal protein S6 kinase beta-2
UniProt Synonym Protein Names
70 kDa ribosomal protein S6 kinase 2; P70S6K2; p70-S6K 2; S6 kinase-related kinase; SRK; Serine/threonine-protein kinase 14B; p70 ribosomal S6 kinase beta
UniProt Gene Name
RPS6KB2
UniProt Synonym Gene Names
STK14B; S6K-beta-2; S6K2; P70S6K2; p70-S6K 2; SRK; S6K-beta; p70 S6 kinase beta; p70 S6K-beta; p70 S6KB; p70-beta
UniProt Entry Name
KS6B2_HUMAN
NCBI Protein Information
ribosomal protein S6 kinase beta-2; P70S6K2; S6K-beta; p70 S6KB; p70-S6K 2; S6K-beta-2; p70 S6K-beta; p70 S6 kinase beta; S6 kinase-related kinase; p70 ribosomal S6 kinase beta; serine/threonine-protein kinase 14B; 70 kDa ribosomal protein S6 kinase 2; serine/threonine-protein kinase 14 beta

NCBI Summary for p70S6Kb

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eucaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation. [provided by RefSeq, Jul 2008]
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UniProt Comments for p70S6Kb

Function: Phosphorylates specifically ribosomal protein S6.

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Subcellular location: Cytoplasm. Nucleus Ref.8 Ref.9.

Post-translational modification: Phosphorylated and activated by MTOR. Phosphorylation by PKC within the NLS in response to mitogenic stimuli causes cytoplasmic retention. Ref.8 Ref.9

Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 protein kinase domain.

Sequence caution: The sequence BAA34402.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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