C9 Recombinant Protein – Cat# MBS1180268

Para-Rep C9 (C9) Recombinant Protein

Name: C9 Recombinant Protein; Para-Rep C9; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS1180268
Price: 970 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1180268

MBS1180268 is a recombinant protein targeting C9. Also known as Recombinant Faba bean necrotic yellows virus Para-Rep C9 (C9). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$970 / 0.02 mg (E-Coli) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Para-Rep C9 (C9), Recombinant Protein

Full Product Name

Recombinant Faba bean necrotic yellows virus Para-Rep C9 (C9)

Product Gene Name

C9 recombinant protein

Sequence

MSAVNWVFTL NFAGEVPVLS FDERVQYAVW QHERVNHDHI QGVIQLKKKA KMNTVKNIIG GNPHLEKMKG SIEEASAYAQ KEESRVAGPW SYGELLKKGS HKRKIMELIK DPENELEEPQ KYRRAMAWSA MDESRKLAEE EGFPYMFYSW QETVLGLLEE EPNDRTIIWV YGPNGNEGKS QFGKFLGLKK DYLYLPGGKT QDMTYMLMKN PKANVVMDIP RCNSEYLNYQ FMELIKNRTI YSYKYEPVGC IINNKIHVIV LANVLPDYEK ISQDRIKIIY C

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Molecular Weight

32,708 Da

Sequence Positions

1-281, Full length protein

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

3D Structure

ModBase 3D Structure for P0CK61

Species

Faba bean necrotic yellows virus (isolate SV292-88) (FBNYV)

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of C9 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for C9. It may not necessarily be applicable to this product.

NCBI GI #

380877123

NCBI Accession #

P0CK61.1

UniProt Primary Accession #

P0CK61

UniProt Secondary Accession #

O91252; P0CAX6; Q9WIK2

NCBI Official Full Name

Para-Rep C9

UniProt Protein Name

Para-Rep C9

UniProt Synonym Protein Names

ATP-dependent helicase C9; Replication-associated protein of non-essential DNA C9

Protein Family

Complement component

UniProt Gene Name

UniProt Synonym Gene Names

Rep9

UniProt Comments for C9

Initiates and terminates the replication only of its own subviral DNA molecule. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities ().

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with C9 recombinant protein

Reference Product	PubMed Publications
FLNA recombinant protein	>12 publications with C9 and FLNA