• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PD-1 Canine Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1884204

Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the… More details

$325 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PD-1, Recombinant Protein
Full Product Name
Canine PD-1 Protein, hFc Tag
Product Gene Name
Product Synonym Names
CD279; PD-1; PD1; SLEB2; hPD-1; hPD-l; hSLE1; Recombinant Canine PD-1 protein with C-terminal human Fc tag
Sequence
PD-1 (Leu25-Gly 168) hFc (Glu99-Ala 330)
Host
HEK293
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Purity/Purification
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Preparation and Storage
Store at -20 degree C to -80 degree C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80 degree C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Molecular Weight
The protein has a predicted molecular mass of 42.3kDa after removal of the signal peptide. The apparent molecular mass of dPD-1-hFc is approximately 55-70kDa due to glycosylation.
Tag
C-Human Fc

Product Categories/Family for PD-1 recombinant protein

SDS-PAGE of PD-1 recombinant protein

Figure 1. Canine PD-1 Protein, hFc Tag on SDS-PAGE under reducing condition.
Canine PD-1 Recombinant Protein – Cat# MBS1884204

ELISA (EIA) of PD-1 recombinant protein

Figure 2. ELISA plate pre-coated by 2 ug/mL (100 uL/well) Canine PD-1 Protein, hFc Tag (MBS1884204) can bind Anti-Canine PD-1(INTERVET 4F12) mAb in a linear range of 0.64–80 ng/mL.
Canine PD-1 Recombinant Protein, ELISA (EIA) – Cat# MBS1884204

ELISA (EIA) of PD-1 recombinant protein

Figure 3. ELISA plate pre-coated by 2 ug/mL (100 uL/well) Canine PD-1 Protein, hFc Tag (MBS1884204) can bind Canine PD-L1 Protein, mFc Tag in a linear range of 16–400 ng/mL.
Canine PD-1 Recombinant Protein, ELISA (EIA) – Cat# MBS1884204

Flow Cytometry (FCM/FACS) of PD-1 recombinant protein

Figure 4. Flow cytometry analysis with 1ug/mL Canine PD-1 Protein, hFc Tag(MBS1884204) on Expi293 cells transfected with Canine PD-L1 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Canine PD-1 Recombinant Protein, Flow Cytometry (FCM/FACS) – Cat# MBS1884204

Other Notes

Small volumes of PD-1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PD-1 purchase

MBS1884204 is a recombinant protein targeting PD-1. Also known as Canine PD-1 Protein, hFc Tag. Host: HEK293.

Additional Details

NCBI/Uniprot data below describe general gene information for PD-1. It may not necessarily be applicable to this product.

NCBI Accession #

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000