• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PLAT Baculovirus Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206493

PLAT, as known as tissue-type plasminogen activator isoform 1, is a secreted serine protease synthesized by endothelial cells. This protein is secreted as a single chain polypeptide precursor which is cleaved in turn by plasmin. Also, it plays a role clinical… More details

$230 / 0.004 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PLAT, Recombinant Protein
Full Product Name
PLAT, 24-562aa, Human, His-tag, Baculovirus
Product Gene Name
Product Synonym Names
Tissue-type plasminogen activator isoform 1; PLAT; T-PA; TPA
Sequence
QEIHARFRRG ARSYQVICRD EKTQMIYQQH QSWLRPVLRS NRVEYCWCNS GRAQCHSVPV KSCSEPRCFN GGTCQQALYF SDFVCQCPEG FAGKCCEIDT RATCYEDQGI SYRGTWSTAE SGAECTNWNS SALAQKPYSG RRPDAIRLGL GNHNYCRNPD RDSKPWCYVF KAGKYSSEFC STPACSEGNS DCYFGNGSAY RGTHSLTESG ASCLPWNSMI LIGKVYTAQN PSAQALGLGK HNYCRNPDGD AKPWCHVLKN RRLTWEYCDV PSCSTCGLRQ YSQPQFRIKG GLFADIASHP WQAAIFAKHR RSPGERFLCG GILISSCWIL SAAHCFQERF PPHHLTVILG RTYRVVPGEE EQKFEVEKYI VHKEFDDDTY DNDIALLQLK SDSSRCAQES SVVRTVCLPP ADLQLPDWTE CELSGYGKHE ALSPFYSERL KEAHVRLYPS SRCTSQHLLN RTVTDNMLCA GDTRSGGPQA NLHDACQGDS GGPLVCLNDG RMTLVGIISW GLGCGQKDVP GVYTKVTNYL DWIRDNMRPH HHHHH
Host
Baculovirus
Form/Format
Liquid. In 50mM MES buffer (pH 5.5) containing 40% glycerol, 5mM CaCl2, 1mM DTT, 0.5M NaCl.
Purity/Purification
> 90% by SDS-PAGE
Concentration
0.25mg/ml (determined by Absorbance at 280nm) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
61.3kDa (545aa) 50-70kDa (SDS-PAGE under reducing conditions.)
3D Structure
ModBase 3D Structure for P00750
Antigen Species
Human
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Applications Tested/Suitable for PLAT recombinant protein
SDS-PAGE

Product References and Citations for PLAT recombinant protein

Chevilley A., et al, (2015) Front Cell Neurosci. 9:415.; Lansley SM., et al, (2015) Am. J. Respir. Cell Mol. Biol. 53:105-112.

Other Notes

Small volumes of PLAT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PLAT purchase

MBS206493 is a recombinant protein targeting PLAT. Also known as PLAT, 24-562aa, Human, His-tag, Baculovirus. Host: Baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for PLAT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tissue-type plasminogen activator isoform 1 preproprotein
NCBI Official Synonym Full Names
plasminogen activator, tissue type
NCBI Official Symbol
PLAT
NCBI Official Synonym Symbols
TPA; T-PA
UniProt Protein Name
Tissue-type plasminogen activator
UniProt Synonym Protein Names
INN: Alteplase; INN: Reteplase
UniProt Gene Name
PLAT
UniProt Synonym Gene Names
t-PA; t-plasminogen activator; tPA
NCBI Protein Information
tissue-type plasminogen activator

NCBI Summary for PLAT

This gene encodes tissue-type plasminogen activator, a secreted serine protease that converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. The encoded preproprotein is proteolytically processed by plasmin or trypsin to generate heavy and light chains. These chains associate via disulfide linkages to form the heterodimeric enzyme. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, while decreased activity leads to hypofibrinolysis, which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]
Show more

UniProt Comments for PLAT

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000