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POLR2K Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206137

POLR2K is one of the smallest subunits of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases. Recombinant human POLR2K protein, fused to His-tag at… More details

$245 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
POLR2K, Recombinant Protein
Full Product Name
POLR2K, 1-58aa, Human, His tag, E Coli
Product Synonym Names
DNA-directed RNA polymerases I II and III subunit RPABC4; DNA-directed RNA polymerases I; II; and III subunit RPABC4; ABC10-alpha; hRPB7.0; hsRPB10a; RPABC4; RPB10alpha; RPB12; RPB7.0
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMDTQKDV QPPKQQPMIY ICGECHTENE IKSRDPIRCR ECGYRIMYKK RTKRLVVFDA R
Host
E Coli
Form/Format
Liquid, In Phosphate buffered saline (pH 7.4) containing 10% glycerol, 1mM DTT
Purity/Purification
> 85% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
9.4 kDa (81aa) confirmed by MALDI-TOF
OMIM
Z47727 mRNA
3D Structure
ModBase 3D Structure for P53803
Antigen Species
Human
Tag Information
His-tag
Applications Tested/Suitable for POLR2K recombinant protein
SDS-PAGE

Product References and Citations for POLR2K recombinant protein

Shpakovski G.V., et al. (1995) Mol. Cell. Biol. 15:4702-4710

Other Notes

Small volumes of POLR2K recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLR2K purchase

MBS206137 is a recombinant protein targeting POLR2K. Also known as POLR2K, 1-58aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for POLR2K. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA-directed RNA polymerases I, II, and III subunit RPABC4
NCBI Official Synonym Full Names
RNA polymerase II subunit K
NCBI Official Symbol
POLR2K
NCBI Official Synonym Symbols
RPB12; RPABC4; RPB7.0; hRPB7.0; hsRPB10a; RPB10alpha; ABC10-alpha
UniProt Protein Name
DNA-directed RNA polymerases I, II, and III subunit RPABC4
UniProt Synonym Protein Names
ABC10-alpha; DNA-directed RNA polymerase II subunit K; RNA polymerase II 7.0 kDa subunit; RPB7.0; RPB10alpha
UniProt Gene Name
POLR2K
UniProt Synonym Gene Names
RNA polymerases I, II, and III subunit ABC4; RPB7.0
NCBI Protein Information
DNA-directed RNA polymerases I, II, and III subunit RPABC4

NCBI Summary for POLR2K

This gene encodes one of the smallest subunits of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases. [provided by RefSeq, Jul 2008]

UniProt Comments for POLR2K

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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