SCO2549 Recombinant Protein – Cat# MBS143200

Protease (SCO2549) Recombinant Protein

Name: SCO2549 Recombinant Protein; Protease; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS143200
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS143200

Description: Protease Recombinant is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease. The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-Phe-Gln/Gly-Pro… More details

$250 / 50 IU +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Protease (SCO2549), Recombinant Protein

Full Product Name

Recombinant Protease

Product Gene Name

SCO2549 recombinant protein

Product Synonym Names

Protease; Recombinant Protease; Protease

Host

E Coli

Form/Format

Sterile filtered colorless solution.

Concentration

1000IU/1.43ml (lot specific)

Preparation and Storage

Protease Recombinant although stable at 4 degree C for 1 week, should be stored desiccated below -18 degree C. Please prevent freeze thaw cycles.

Molecular Weight

115,647 Da

Cleavage Buffer

50mM Tris-HCl, pH-7.0 (at 25 degree C), 150mM NaCl, 1mM EDTA, 1mM dithiothreitol. Chill to 5 degree C prior to use.

Cleavage Conditions

For Cleavage of a Fusion Protein: During cleavage reactions, it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield, purity, and extent of digestion. The amount of PreScission Protease, temperature and length of incubation required for complete digestion of a given GST fusion partner may vary depending on the fusion partner. Optimal conditions for each fusion should be determined in pilot experiments. Digestion may be improved by adding TritonTM X-100, TweenTM 20, NonidetTM, or NP40 to a concentration of 0.01%. Concentrations of these detergents up to 1% do not inhibit PreScission Protease.

Unit Defenition

One unit will cleave ?90% of 100 ug of a test GST-fusion protein in Cleavage Buffer (50mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.0 at 25 degree C) at 5 degree C for 16 hours.

Product Categories/Family for SCO2549 recombinant protein

Enzymes; Protease

Other Notes

Small volumes of SCO2549 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SCO2549 purchase

MBS143200 is a recombinant protein targeting SCO2549. Also known as Recombinant Protease. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for SCO2549. It may not necessarily be applicable to this product.

NCBI GI #

21221008

NCBI GeneID

1097983

NCBI Accession #

NP_626787.1

UniProt Related Accession #

Q9RDE2

GenBank Nucleotide #

NC_003888.3

NCBI Official Full Name

protease

NCBI Official Symbol

SCO2549

NCBI Official Synonym Symbols

SCC77.16c.

UniProt Protein Name

Tricorn protease homolog 1

Protein Family

Protease

UniProt Gene Name

tri1

UniProt Entry Name

TRI1_STRCO

NCBI Protein Information

protease

UniProt Comments for SCO2549

Degrades oligopeptides in a sequential manner.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.