3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.

RPN2 Recombinant Protein

Name: RPN2 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS205495
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205495

RPN2, also known as ribophorin 2, is a dolichyl-diphosphooligosaccharide protein glycosyltransferase subunit 2. This protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

RPN2, Recombinant Protein

Full Product Name

RPN2, 23-540aa, Human, His tag, E Coli

Product Gene Name

RPN2 recombinant protein

Product Synonym Names

Ribophorin II; SWP1; RPNII; RIBIIR; RPN-II

Sequence

MGSSHHHHHH SSGLVPRGSH MLTPTHYLTK HDVERLKASL DRPFTNLESA FYSIVGLSSL GAQVPDAKKA CTYIRSNLDP SNVDSLFYAA QASQALSGCE ISISNETKDL LLAAVSEDSS VTQIYHAVAA LSGFGLPLAS QEALSALTAR LSKEETVLAT VQALQTASHL SQQADLRSIV EEIEDLVARL DELGGVYLQF EEGLETTALF VAATYKLMDH VGTEPSIKED QVIQLMNAIF SKKNFESLSE AFSVASAAAV LSHNRYHVPV VVVPEGSASD THEQAILRLQ VTNVLSQPLT QATVKLEHAK SVASRATVLQ KTSFTPVGDV FELNFMNVKF SSGYYDFLVE VEGDNRYIAN TVELRVKIST EVGITNVDLS TVDKDQSIAP KTTRVTYPAK AKGTFIADSH QNFALFFQLV DVNTGAELTP HQTFVRLHNQ KTGQEVVFVA EPDNKNVYKF ELDTSERKIE FDSASGTYTL YLIIGDATLK NPILWNVADV VIKFPEEEAP STVLSQNLFT PKQEIQHLFR EPEKRPPTV

Host

E Coli

Form/Format

Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Purity/Purification

> 90% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4°C short term (1-2 weeks).
For long term storage, aliquot and store at -20°C or -70°C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

59.2kDa (539aa)

OMIM

Y00282 mRNA

3D Structure

ModBase 3D Structure for P04844

Tag Information

His-tag

Product Categories/Family for RPN2 recombinant protein

Enzymes & Proteases

Applications Tested/Suitable for RPN2 recombinant protein

SDS-PAGE

Product References and Citations for RPN2 recombinant protein

Skarnes WC. et al. (2011) Nature. 474:337-342.; Stoffel M. et al. (1992) Hum Mol Genet. 1:656.

Other Notes

Small volumes of RPN2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RPN2 purchase

MBS205495 is a recombinant protein targeting RPN2. Also known as RPN2, 23-540aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for RPN2. It may not necessarily be applicable to this product.

NCBI GI #

35493916

NCBI GeneID

6185

NCBI Accession #

NP_002942

UniProt Primary Accession #

P04844

UniProt Secondary Accession #

Q5JYR6; Q6IBA5; Q96E21; Q9BUQ3; Q9UBE1

UniProt Related Accession #

P04844

GenBank Nucleotide #

NM_002951.4

NCBI Official Full Name

dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 isoform 1

NCBI Official Synonym Full Names

ribophorin II

NCBI Official Symbol

RPN2

NCBI Official Synonym Symbols

SWP1; RPNII; RIBIIR; RPN-II

UniProt Protein Name

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2

UniProt Synonym Protein Names

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit; RIBIIR; Ribophorin II; RPN-II; Ribophorin-2

Protein Family

26S proteasome regulatory

UniProt Gene Name

RPN2

UniProt Synonym Gene Names

RPN-II

NCBI Protein Information

dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2

NCBI Summary for RPN2

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

UniProt Comments for RPN2

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.

Research Articles on RPN2

1. RPN2 expression correlates with gastric adenocarcinoma cell invasion and shows promise as a new prognostic factor in human gastric adenocarcinoma

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with RPN2 recombinant protein

Reference Product	PubMed Publications
RPN1 recombinant protein	>17 publications with RPN2 and RPN1
STT3A recombinant protein	>1 publications with RPN2 and STT3A

Pathways associated with RPN2 recombinant protein

Products by Pathway	Pathway Diagram
Asparagine N-linked Glycosylation Pathway antibodies	Asparagine N-linked Glycosylation Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
N-Glycan Biosynthesis Pathway antibodies	N-Glycan Biosynthesis Pathway Diagram
N-Glycan Biosynthesis Pathway antibodies	N-Glycan Biosynthesis Pathway Diagram
N-glycosylation By Oligosaccharyltransferase Pathway antibodies	N-glycosylation By Oligosaccharyltransferase Pathway Diagram
N-glycosylation By Oligosaccharyltransferase Pathway antibodies	N-glycosylation By Oligosaccharyltransferase Pathway Diagram
Post-translational Protein Modification Pathway antibodies	Post-translational Protein Modification Pathway Diagram
Proteasome Degradation Pathway antibodies	Proteasome Degradation Pathway Diagram

Diseases associated with RPN2 recombinant protein

Disease Name	Pubmed Publications
Neoplasm Metastasis Antibodies	>7 publications with RPN2 and Neoplasm Metastasis
Nervous System Diseases Antibodies	>4 publications with RPN2 and Nervous System Diseases
Ovarian Diseases Antibodies	>2 publications with RPN2 and Ovarian Diseases
Inflammation Antibodies	>2 publications with RPN2 and Inflammation
Cell Transformation, Neoplastic Antibodies	>2 publications with RPN2 and Cell Transformation, Neoplastic
Hypertrophy Antibodies	>1 publications with RPN2 and Hypertrophy
Heart Diseases Antibodies	>1 publications with RPN2 and Heart Diseases
Congenital Abnormalities Antibodies	>1 publications with RPN2 and Congenital Abnormalities

Organs/Tissues associated with RPN2 recombinant protein

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>4 publications with RPN2 and Lung
Eye Antibodies	>3 publications with RPN2 and Eye
Lymph Antibodies	>3 publications with RPN2 and Lymph
Mouth Antibodies	>3 publications with RPN2 and Mouth
Lymph Node Antibodies	>3 publications with RPN2 and Lymph Node
Blood Antibodies	>3 publications with RPN2 and Blood
Muscle Antibodies	>2 publications with RPN2 and Muscle
Pancreas Antibodies	>2 publications with RPN2 and Pancreas
Stomach Antibodies	>2 publications with RPN2 and Stomach
Brain Antibodies	>2 publications with RPN2 and Brain