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Selectin, Endothelium (SELE) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2012252
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MBS2012252 is a recombinant protein targeting SELE. Also known as Recombinant Selectin, Endothelium (SELE). Host: E Coli. More details

$225 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
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Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Selectin, Endothelium (SELE), Recombinant Protein
Full Product Name
Recombinant Selectin, Endothelium (SELE)
Product Gene Name
SELE recombinant protein
Sequence
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MRNKKFEL GLEFPNLPYY IDGDVKLTQS MAIIRYIADK HNMLGGCPKE RAEISMLEGA VLDIRYGVSR IAYSKDFETL KVDFLSKLPE MLKMFEDRLC HKTYLNGDHV THPDFMLYDA LDVVLYMDPM CLDAFPKLVC FKKRIEAIPQ IDKYLKSSKY IAWPLQGWQA TFGGGDHPPK SDGSTSGSGH HHHHHSAGLV PRGSTAIGMK ETAAAKFERQ HMDSPDLGTL EVLFQGPLGS EF-E FAYKSTCHFT CAEGFGLQGP AQIECTAQGQ WTQQAPVCKA VKCPAVSQPK NGLVKFTHSP TGEFTYKSSC AFSCEEGFEL RGSAQLACTS QGQWTQEVPS CQVVQCSSLE VPREINMSCS GEPVFGAVCT FACPEGWMLN GSVALTCGAT GHWSGMLPTC
Host
E Coli
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Purity/Purification
> 95%
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: SELE (MBS2001476)
Immunogen: SELE (MBS2012252)
Matching Pairs
Biotin Conjugated Antibody: SELE (MBS2005677)
Immunogen: SELE (MBS2012252)
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Molecular Weight
52,567 Da
Chromosome Location
Chromosome: 4; NC_010446.4 (88914284..88924075). Location: 4; 4
3D Structure
ModBase 3D Structure for P98110
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Organism
Sus scrofa; Porcine (Pig)
Expression System
Prokaryotic expression
Residues
Glu260~Cys420 (Accession # P98110) with two N-terminal Tags, His-tag and GST-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for SELE recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

SDS-Page of SELE recombinant protein

SELE Recombinant Protein – Cat# MBS2012252

Other Notes

Small volumes of SELE recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SELE. It may not necessarily be applicable to this product.

NCBI GI #
47523602
NCBI GeneID
397508
NCBI Accession #
NP_999433.1
UniProt Primary Accession #
P98110
UniProt Related Accession #
P98110
GenBank Nucleotide #
NM_214268.1
NCBI Official Full Name
E-selectin
NCBI Official Symbol
SELE
UniProt Protein Name
E-selectin
UniProt Synonym Protein Names
CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2
Protein Family
E-selectin
UniProt Gene Name
SELE
UniProt Synonym Gene Names
ELAM-1; LECAM2
UniProt Entry Name
LYAM2_PIG
NCBI Protein Information
E-selectin; ELAM-1; LECAM2; CD62 antigen-like family member E; endothelial leukocyte adhesion molecule 1; selectin E (endothelial adhesion molecule 1); leukocyte-endothelial cell adhesion molecule 2

UniProt Comments for SELE

Function: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.

Subunit structure: Interacts with PSGL1/SELPLG and PODXL2 through the sialyl Lewis X epitope. PSGL1 sulfation appears not to be required for this interaction

By similarity.

Subcellular location: Membrane; Single-pass type I membrane protein.

Miscellaneous: Important in acute cellular allograft rejection and probably also in xenograft rejection.

Sequence similarities: Belongs to the selectin/LECAM family.Contains 1 C-type lectin domain.Contains 1 EGF-like domain.Contains 4 Sushi (CCP/SCR) domains.
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Research Articles on SELE

1. Increased E-selectin mRNA at end of 20 hours of cold ischemia time might indicate a preactivated state of endothelial cells potentially triggered by bacterial translocation or products.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SELE recombinant protein

 Reference Product  PubMed Publications
 ICAM1 recombinant protein  >68 publications with SELE and ICAM1
 TNF recombinant protein  >29 publications with SELE and TNF
 VCAM1 recombinant protein  >9 publications with SELE and VCAM1
 VWF recombinant protein  >6 publications with SELE and VWF
 RELA recombinant protein  >3 publications with SELE and RELA
 NFKB1 recombinant protein  >2 publications with SELE and NFKB1

Pathways associated with SELE recombinant protein

 Products by Pathway  Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram
 TNF Signaling Pathway antibodies  TNF Signaling Pathway Diagram
 TNF Signaling Pathway antibodies  TNF Signaling Pathway Diagram

Diseases associated with SELE recombinant protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >76 publications with SELE and Inflammation
 Cardiovascular Diseases Antibodies  >67 publications with SELE and Cardiovascular Diseases
 Necrosis Antibodies  >36 publications with SELE and Necrosis
 Atherosclerosis Antibodies  >27 publications with SELE and Atherosclerosis
 Lung Diseases Antibodies  >20 publications with SELE and Lung Diseases
 Heart Diseases Antibodies  >17 publications with SELE and Heart Diseases
 Myocardial Ischemia Antibodies  >16 publications with SELE and Myocardial Ischemia
 Hypertension Antibodies  >11 publications with SELE and Hypertension
 Myocardial Infarction Antibodies  >7 publications with SELE and Myocardial Infarction
 Dyslipidemias Antibodies  >4 publications with SELE and Dyslipidemias

Organs/Tissues associated with SELE recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Lymph Node Antibodies  >5 publications with SELE and Lymph Node
 Placenta Antibodies  >3 publications with SELE and Placenta
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