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SETD3 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS389328

Short Description: Full-length human SETD3 protein (accession number NP_115609.2 was expressed in a baculovirus expression system and contains an N-terminal DYKDDDDK-Tag. The molecular weight of SETD3 is 68.5 kDa. Recombinant SETD3 protein is suitable for use… More details

$690 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SETD3, Recombinant Protein
Full Product Name
Recombinant SETD3 protein
Product Gene Name
SETD3 recombinant protein
Product Synonym Names
SET Domain Containing 3; Actin Histidine Methyltransferase; C14orf154; EC 2.1.1.85
Host
Baculovirus
Form/Format
Recombinant SETD3 protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Molecular Weight
32,540 Da
OMIM
615671
3D Structure
ModBase 3D Structure for Q86TU7
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
Recombinant SETD3 protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for SETD3 recombinant protein

Recombinant Proteins; Histone Methyltransferase (HMT); Transcription

SDS-Page of SETD3 recombinant protein

Recombinant SETD3 protein gel 10% SDS-PAGE Coomassie staining MW: 68.5 kDa Purity: >90%
SETD3 Recombinant Protein – Cat# MBS389328

Testing Data of SETD3 recombinant protein

MTase-Glo Assay for Recombinant SETD3 protein methyltransferase activity 1 uM beta-actin peptide (66-TLKYPIEHGIVTNWD-80) and 1 uM SAM were incubated with different concentrations of recombinant SETD3 protein in an 8 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP for 1 hr. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added and luminescence were read after another 30 min incubation. SAH standard curve (0-1 uM) was performed following the same protocol.
SETD3 Recombinant Protein, Testing Data – Cat# MBS389328

Testing Data of SETD3 recombinant protein

MTase-Glo Assay for Recombinant SETD3 protein methyltransferase activity 1 uM beta-actin peptide (66-TLKYPIEHGIVTNWD-80) and 1 uM SAM were incubated with different concentrations of recombinant SETD3 protein in an 8 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP for 1 hr. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added and luminescence were read after another 30 min incubation. SAH standard curve (0-1 uM) was performed following the same protocol.
SETD3 Recombinant Protein, Testing Data – Cat# MBS389328

Other Notes

Small volumes of SETD3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SETD3 purchase

MBS389328 is a recombinant protein targeting SETD3. Also known as Recombinant SETD3 protein. Host: Baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for SETD3. It may not necessarily be applicable to this product.

NCBI GI #
40068481
NCBI GeneID
84193
NCBI Accession #
NP_115609.2
UniProt Primary Accession #
Q86TU7
UniProt Secondary Accession #
Q0VAQ2; Q659C0; Q86TU8; Q96GY9; Q9H5U5; A0PJU3; A5PLP0; B4DZE8
UniProt Related Accession #
Q86TU7
GenBank Nucleotide #
NM_032233.2
NCBI Official Full Name
histone-lysine N-methyltransferase setd3 isoform a
NCBI Official Synonym Full Names
SET domain containing 3
NCBI Official Symbol
SETD3
NCBI Official Synonym Symbols
C14orf154
UniProt Protein Name
Histone-lysine N-methyltransferase setd3
UniProt Synonym Protein Names
SET domain-containing protein 3
Protein Family
Histone-lysine N-methyltransferase
UniProt Gene Name
SETD3
UniProt Synonym Gene Names
C14orf154
UniProt Entry Name
SETD3_HUMAN
NCBI Protein Information
histone-lysine N-methyltransferase setd3

UniProt Comments for SETD3

SETD3: Histone methyltransferase that methylates 'Lys-36' of histone H3 (H3K36me). H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation. Belongs to the SETD3 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; EC 2.1.1.43; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 14q32.2

Cellular Component: nuclear chromatin

Molecular Function: histone lysine N-methyltransferase activity (H3-K4 specific); transcription coactivator activity; histone lysine N-methyltransferase activity (H3-K36 specific)

Biological Process: transcription, DNA-dependent; peptidyl-lysine di-methylation; positive regulation of transcription, DNA-dependent; peptidyl-lysine tri-methylation; histone H3-K4 methylation; peptidyl-lysine mono-methylation; positive regulation of muscle cell differentiation; histone H3-K36 methylation; positive regulation of transcription from RNA polymerase II promoter
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with SETD3 recombinant protein

 Products by Pathway  Pathway Diagram
 Chromatin Modifying Enzymes Pathway antibodies  Chromatin Modifying Enzymes Pathway Diagram
 Chromatin Organization Pathway antibodies  Chromatin Organization Pathway Diagram
 PKMTs Methylate Histone Lysines Pathway antibodies  PKMTs Methylate Histone Lysines Pathway Diagram

Diseases associated with SETD3 recombinant protein

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >1 publications with SETD3 and Carcinoma

Organs/Tissues associated with SETD3 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1 publications with SETD3 and Muscle
 Blood Antibodies  >1 publications with SETD3 and Blood
 Brain Antibodies  >1 publications with SETD3 and Brain
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