• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

SPHK2 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515665

Description: Recombinant full-length human SPHK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: SPHK2 is a member of the SPHK family and catalyzes the phosphorylation of the lipid sphingosine, creating the… More details

$505 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SPHK2, Recombinant Protein
Full Product Name
SPHK2, Active
Product Gene Name
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
~110 kDa
Sequence Positions
Full length
OMIM
AF245447 mRNA
3D Structure
ModBase 3D Structure for Q9NRA0
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Applications Tested/Suitable for SPHK2 recombinant protein
Kinase Assay

Testing Data #2 of SPHK2 recombinant protein

SPHK2 Recombinant Protein, Testing Data #2 – Cat# MBS515665

Product References and Citations for SPHK2 recombinant protein

1. Mizugishi, K. et al: Maternal disturbance in activated sphingolipid metabolism causes pregnancy loss in mice. J. Clin. Invest. 1172993-3006, 2007. 2. Hait, N. C.et al: Regulation of histone acetylation in the nucleus by sphingosine-1-phosphate. Science 3251254-1257, 2009. NoteErratumScience 326366 only, 2009.

Other Notes

Small volumes of SPHK2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPHK2 purchase

MBS515665 is a recombinant protein targeting SPHK2. Also known as SPHK2, Active. Host: Sf9 insect cells using baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for SPHK2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 1, mRNA
NCBI Official Synonym Full Names
sphingosine kinase 2
NCBI Official Symbol
SPHK2
NCBI Official Synonym Symbols
SK 2; SK-2; SPK 2; SPK-2
UniProt Protein Name
Sphingosine kinase 2
Protein Family
UniProt Gene Name
SPHK2
UniProt Synonym Gene Names
SK 2; SPK 2
NCBI Protein Information
sphingosine kinase 2

NCBI Summary for SPHK2

This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
Show more

UniProt Comments for SPHK2

SPHK2: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro- dihydrosphingosine, D-erythro-sphingosine and L-threo- dihydrosphingosine. Binds phosphoinositides. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.91; Kinase, lipid; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: cytosol; intracellular membrane-bound organelle; lysosomal membrane; membrane

Molecular Function: D-erythro-sphingosine kinase activity; protein binding; sphinganine kinase activity

Biological Process: sphingolipid biosynthetic process; sphingosine biosynthetic process
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SPHK2 recombinant protein

 Reference Product  PubMed Publications
 SGPP1 recombinant protein  >2 publications with SPHK2 and SGPP1
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000