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SUV420H2 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS389075

Short Description: Recombinant SUV420H2 protein was expressed in E Coli as full-length protein (accession number NP_116090.2) and contains an N-terminal GST-Tag with an observed molecular weight of 78.4 kDa. It is suitable for use in histone methyltransferase… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SUV420H2, Recombinant Protein
Full Product Name
Recombinant SUV420H2 protein
Product Gene Name
SUV420H2 recombinant protein
Product Synonym Names
KMT5C
Host
E Coli
Form/Format
Recombinant SUV420H2 protein is supplied at a concentration of 0.3ug/ul in 25mM Tris pH8.0, 300mM NaCl, 5% glycerol.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Molecular Weight
52,113 Da
OMIM
613198
3D Structure
ModBase 3D Structure for Q86Y97
Protein Species
Human
Tag
GST-Tag
Notes
Recombinant SUV420H2 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HMT Assay Conditions: 2ug Recombinant Nucleosomes (H3.1) were incubated with 0ug (-), 0.1ug (+), 0.2ug (++), 0.4ug (+++) SUV420H2 protein, in 30ul reaction containing 50mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2mM MgCl2, 1mM TCEP, 50uM SAM for 3 hr at RT. Products run on a 12.5% SDS-PAGE gel were detected with anti-H4K20me2 or anti-H4K20me3. SUV420H2 only was used as negative control.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for SUV420H2 recombinant protein

Recombinant Proteins; Histone Methyltransferase (HMT)

SDS-Page of SUV420H2 recombinant protein

Recombinant SUV420H2 protein 10% SDS-PAGE Coomassie staining MW: 78.4 kDa Purity: > 60%
SUV420H2 Recombinant Protein – Cat# MBS389075

SDS-Page of SUV420H2 recombinant protein

Recombinant SUV420H2 activity assay Recombinant Nucleosomes (H3.1) in various amounts were incubated with SUV420H2 protein. Reaction products were run on SDS-PAGE gel and detected with anti-H4K20me2 or anti-H4K20me3. The activity of SUV420H2 with nucleosomes as substrate is better than with histone octamers as substrate.
SUV420H2 Recombinant Protein, SDS-Page – Cat# MBS389075

Other Notes

Small volumes of SUV420H2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SUV420H2 purchase

MBS389075 is a recombinant protein targeting SUV420H2. Also known as Recombinant SUV420H2 protein. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for SUV420H2. It may not necessarily be applicable to this product.

NCBI GI #
31543169
NCBI GeneID
84787
NCBI Accession #
NP_116090.2
UniProt Primary Accession #
Q86Y97
UniProt Secondary Accession #
Q8WZ10; Q9BRZ6
UniProt Related Accession #
Q86Y97
GenBank Nucleotide #
NM_032701.3
NCBI Official Full Name
histone-lysine N-methyltransferase SUV420H2
NCBI Official Synonym Full Names
suppressor of variegation 4-20 homolog 2 (Drosophila)
NCBI Official Symbol
SUV420H2
NCBI Official Synonym Symbols
KMT5C
UniProt Protein Name
Histone-lysine N-methyltransferase SUV420H2
UniProt Synonym Protein Names
Lysine N-methyltransferase 5C; Suppressor of variegation 4-20 homolog 2
UniProt Gene Name
SUV420H2
UniProt Synonym Gene Names
KMT5C
UniProt Entry Name
SV422_HUMAN
NCBI Protein Information
histone-lysine N-methyltransferase SUV420H2; suv4-20h2; su(var)4-20 homolog 2; lysine N-methyltransferase 5C

UniProt Comments for SUV420H2

SUV420H2: Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Belongs to the histone-lysine methyltransferase family. Suvar4-20 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: nucleoplasm; centric heterochromatin; nuclear heterochromatin; condensed nuclear chromosome, pericentric region

Molecular Function: protein binding; histone lysine N-methyltransferase activity (H4-K20 specific)

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with SUV420H2 recombinant protein

 Products by Pathway  Pathway Diagram
 Lysine Degradation Pathway antibodies  Lysine Degradation Pathway Diagram
 Lysine Degradation Pathway antibodies  Lysine Degradation Pathway Diagram

Diseases associated with SUV420H2 recombinant protein

 Disease Name  Pubmed Publications
 Hyperglycemia Antibodies  >1 publications with SUV420H2 and Hyperglycemia

Organs/Tissues associated with SUV420H2 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >1 publications with SUV420H2 and Eye
 Muscle Antibodies  >1 publications with SUV420H2 and Muscle
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