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UBE2D4 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS389694

UBE2D4 (Ubiquitin-conjugating enzyme E2 D4) also known as HBUCE1. UBE2D4 is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair and plays an important role in various cellular processes. UBE2D4 can… More details

$690 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
UBE2D4, Recombinant Protein
Full Product Name
Recombinant UBE2D4 protein
Product Synonym Names
Ubiquitin conjugating enzyme E2 D4; HBUCE1; UBCH5D
Host
E coli
Form/Format
Recombinant UBE2D4 protein is supplied in 25 mM Tris 7.4, 300mM NaCl, 20% glycerol, 0.5 mM TCEP.
Purity/Purification
>=95% by SDS-PAGE.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Molecular Weight
18.81kDa
Protein Species
Human
Protein Details
Recombinant UBE2D4 protein that includes full length of human UBE2D4 protein (accession number NP_057067.1) was expressed in E coli and contains an N-terminal His tag with a molecular weight of 18.81kDa.
Application Notes for UBE2D4 recombinant protein
Lab has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product.  All available data for this product is shown.

SDS-Page of UBE2D4 recombinant protein

Recombinant UBE2D4 protein gel. UBE2D4 protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 18.81kDa Purity: >95%
UBE2D4 Recombinant Protein – Cat# MBS389694

Testing Data of UBE2D4 recombinant protein

AMP-Glo assay for UBE2D4 activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2D4 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence were read after another 30 min incubation.
UBE2D4 Recombinant Protein, Testing Data – Cat# MBS389694

Other Notes

Small volumes of UBE2D4 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBE2D4 purchase

MBS389694 is a recombinant protein targeting UBE2D4. Also known as Recombinant UBE2D4 protein. Host: E coli.

Additional Details

NCBI/Uniprot data below describe general gene information for UBE2D4. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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