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UHRF1 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS389077

Short Description: Recombinant UHRF1 protein was expressed in E Colii cells as the full-length protein (accession number NP_037414.3) with a C-terminal 6xHis-tag. The molecular weight of the protein is 93.1 kDa. It is suitable for analysis of enzyme function… More details

$700 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
UHRF1, Recombinant Protein
Full Product Name
Recombinant UHRF1 protein
Product Gene Name
UHRF1 recombinant protein
Product Synonym Names
Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF
Host
E Coli
Form/Format
Recombinant UHRF1 protein is supplied at a concentration of 1ug/ul in 25mM Tris pH8.0, 300mM NaCl, 5% glycerol.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Molecular Weight
91,116 Da
OMIM
607990
3D Structure
ModBase 3D Structure for Q96T88
Protein Species
Human
Tag
His-Tag
Notes
This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for UHRF1 recombinant protein

Recombinant Proteins; DNA Damage; Histone Deacetylase (HDAC)

SDS-Page of UHRF1 recombinant protein

Recombinant UHRF1 protein gel 8% SDS-PAGE Coomassie staining MW: 93.1 kDa Purity: > 60%
UHRF1 Recombinant Protein – Cat# MBS389077

Testing Data of UHRF1 recombinant protein

UHRF1 binding assay1 uM H3K9me3 peptide was incubated with different concentrations of UHRF1 protein in 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-His antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
UHRF1 Recombinant Protein, Testing Data – Cat# MBS389077

Testing Data of UHRF1 recombinant protein

UHRF1 Recombinant Protein, Testing Data – Cat# MBS389077

Other Notes

Small volumes of UHRF1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UHRF1 purchase

MBS389077 is a recombinant protein targeting UHRF1. Also known as Recombinant UHRF1 protein. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for UHRF1. It may not necessarily be applicable to this product.

NCBI GI #
115430233
NCBI GeneID
29128
NCBI Accession #
NP_037414.3
UniProt Primary Accession #
Q96T88
UniProt Secondary Accession #
Q2HIX7; Q8J022; Q9H6S6; Q9P115; Q9P1U7; A0JBR2; A8K024; B2RBA9
UniProt Related Accession #
Q96T88
GenBank Nucleotide #
NM_013282.3
NCBI Official Full Name
E3 ubiquitin-protein ligase UHRF1 isoform 2
NCBI Official Synonym Full Names
ubiquitin like with PHD and ring finger domains 1
NCBI Official Symbol
UHRF1
NCBI Official Synonym Symbols
Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF1; huNp95
UniProt Protein Name
E3 ubiquitin-protein ligase UHRF1
UniProt Synonym Protein Names
Inverted CCAAT box-binding protein of 90 kDa; Nuclear protein 95; Nuclear zinc finger protein Np95; HuNp95; hNp95; RING finger protein 106; Transcription factor ICBP90; Ubiquitin-like PHD and RING finger domain-containing protein 1; hUHRF1; Ubiquitin-like-containing PHD and RING finger domains protein 1
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
UHRF1
UniProt Synonym Gene Names
ICBP90; NP95; RNF106; HuNp95; hNp95; hUHRF1
UniProt Entry Name
UHRF1_HUMAN
NCBI Protein Information
E3 ubiquitin-protein ligase UHRF1

NCBI Summary for UHRF1

This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12. [provided by RefSeq, Feb 2014]
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Research Articles on UHRF1

1. These results suggest that tumor-promoting functions of UHRF1 in retinoblastoma are largely independent of its role in DNA methylation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with UHRF1 recombinant protein

 Products by Pathway  Pathway Diagram
 DNA Methylation Pathway antibodies  DNA Methylation Pathway Diagram
 Epigenetic Regulation Of Gene Expression Pathway antibodies  Epigenetic Regulation Of Gene Expression Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Pathways Affected In Adenoid Cystic Carcinoma antibodies  Pathways Affected In Adenoid Cystic Carcinoma Diagram
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