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ULP1 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9719067

Background: SUMO (Small Ubiquitin-like MOdifiers) Protease 1 (Ulp1, Ubl-specific protease 1 from Saccharomyces cerevisiae) is a highly active cysteine protease. It is highly specific as it recognizes the tertiary structure of the ubiquitin-like (UBL) protein,… More details

$140 / 500 Units +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ulp1, Recombinant Protein
Full Product Name
Ulp1 (SUMO Protease/Ulp1 peptidase)
Product Gene Name
Ulp1 recombinant protein
Product Synonym Names
Ulp1 peptidase; SUMO Protease
Host
E Coli
Form/Format
Lyophilized from sterile 20 mM Tris, 500 mM NaCl pH8.0.
Purity/Purification
>90% as determined by SDS-PAGE
Preparation and Storage
Lyophilized ULP1 protein product should be stored desiccated below -80 degree C. Avoid freezing and thawing.
Shipping: The product is shipped with blue ice.
Sequence Positions
219aa
3D Structure
ModBase 3D Structure for Q02724
Protein Construction
Amino acid sequence derived from ULP1 isoform (Q02724) (403-621 aa + N-terminal Poly-6*His tag C-terminal Poly-6*His tag) was expressed.
Protein Length
The recombinant human ULP1 consists of 219 amino acids and has a predicted molecular mass of 28.7 kDa. The apparant molecular mass of ULP1 is approximately 28 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Unit Definition
One unit of SUMO Protease is defined as the amount of enzyme needed to cleave 85% of 2ug of substrate protein at 30°C in one hour.
Enzyme Digestion Conditions
4°C enzyme digestion overnight is recommended, users can explore according to their own research target protein. After digestion, a small amount of samples can be taken for SDS-PAGE analysis. To remove the ULP1 in the digested system, use His tag purification resin affinity chromatography.

Product Categories/Family for Ulp1 recombinant protein

Cell Culture
Application Notes for Ulp1 recombinant protein
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized ULP1 to 5 U/uL using the buffer we provided, which can then be further diluted to other aqueous solutions.
Fusion Protein 1000 ug 10X SUMO Protease Buffer 20 uL
SUMO Protease (10 units) 2 uL
Total volume 1000 uL

SDS-PAGE of Ulp1 recombinant protein

ULP1 Recombinant Protein – Cat# MBS9719067

SDS-PAGE of Ulp1 recombinant protein

SDS-PAGE analysis of ULP1.
ULP1 Recombinant Protein, SDS-PAGE – Cat# MBS9719067

Other Notes

Small volumes of Ulp1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ulp1 purchase

MBS9719067 is a recombinant protein targeting Ulp1. Also known as Ulp1 (SUMO Protease/Ulp1 peptidase). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for Ulp1. It may not necessarily be applicable to this product.

NCBI GI #
6325237
NCBI GeneID
856087
NCBI Accession #
NP_015305.1
UniProt Primary Accession #
Q02724
UniProt Secondary Accession #
D6W3Z2
UniProt Related Accession #
Q02724
GenBank Nucleotide #
NM_001183834.1
NCBI Official Full Name
SUMO protease ULP1
NCBI Official Symbol
ULP1
NCBI Official Synonym Symbols
NIB1
UniProt Protein Name
Ubiquitin-like-specific protease 1
Protein Family
Ubiquitin-like-specific protease
UniProt Gene Name
ULP1
NCBI Protein Information
SUMO protease ULP1

UniProt Comments for Ulp1

Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SMT3 to its mature form and deconjugation of SMT3 from targeted proteins. Has an essential role in the G2/M phase of the cell cycle.

Research Articles on Ulp1

1. our data highlight a role for the NPC-associated SUMO protease Ulp1 in regulating the sumoylation of gene-bound transcription regulators.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with Ulp1 recombinant protein

 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Processing And Activation Of SUMO Pathway antibodies  Processing And Activation Of SUMO Pathway Diagram
 SUMO Is Proteolytically Processed Pathway antibodies  SUMO Is Proteolytically Processed Pathway Diagram
 SUMOylation Pathway antibodies  SUMOylation Pathway Diagram
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