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Fibroblast Growth Factor-Basic (rFGF 2) Rat Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS143301

Description: bFGF Rat Recombinant (FGF-2) produced in E Coli is a single, non-glycosylated, polypeptide chain containing 145 amino acids and having a molecular mass of 16.3 kDa.The bFGF2 is purified by proprietary chromatographic techniques.Introduction:… More details

$250 / 0.01 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Fibroblast Growth Factor-basic (rFGF 2), Active Protein
Full Product Name
Recombinant Rat Fibroblast Growth Factor-basic
Product Gene Name
rFGF 2 active protein
Product Synonym Names
FGF 2 Rat; Fibroblast Growth Factor-basic Rat Recombinant; HBGH-2; HBGF-2; Prostatropin; FGF-2; FGB-b; Fibroblast Growth Factor-basic; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2
Sequence
MPALPEDGGG A FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
Host
E Coli
Form/Format
FGF-b was lyophilized from 1 mg/ml solution after extensive dialysis against 20mM phosphate buffer, pH 7.4 and 150mM NaCl.
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity/Purification
Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Preparation and Storage
Lyophilized Rat bFGF although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Rat FGF-2 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Molecular Weight
17,139 Da
3D Structure
ModBase 3D Structure for P13109
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility
It is recommended to reconstitute the lyophilized Rat bFGF in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
The ED50 range as determined by the dose-dependant proliferation of BALB/3T3 cells was found to be less than 0.2 ng/ml, corresponding to a specific activity of 5,000,000 IU/mg.

Product Categories/Family for rFGF 2 active protein

Cytokines and Growth Factors; Growth Factors; Fibroblast Growth Factor

Other Notes

Small volumes of rFGF 2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for rFGF 2 purchase

MBS143301 is an active protein targeting rFGF 2. Also known as Recombinant Rat Fibroblast Growth Factor-basic. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for rFGF 2. It may not necessarily be applicable to this product.

NCBI GI #
9506599
NCBI GeneID
54250
NCBI Accession #
NP_062178.1
UniProt Primary Accession #
P13109
UniProt Related Accession #
P13109
GenBank Nucleotide #
NM_019305.2
NCBI Official Full Name
fibroblast growth factor 2
NCBI Official Synonym Full Names
fibroblast growth factor 2
NCBI Official Symbol
Fgf2
NCBI Official Synonym Symbols
bFGF; Fgf-2
UniProt Protein Name
Fibroblast growth factor 2
UniProt Synonym Protein Names
Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2
UniProt Gene Name
Fgf2
UniProt Synonym Gene Names
Fgf-2; FGF-2; bFGF; HBGF-2
UniProt Entry Name
FGF2_RAT
NCBI Protein Information
fibroblast growth factor 2; HBGF-2; basic fibroblast growth factor; heparin-binding growth factor 2

NCBI Summary for rFGF 2

activates the MAP kinase signaling pathway; plays a role in synaptic transmission; induces cell proliferation [RGD, Feb 2006]

UniProt Comments for rFGF 2

FGF2: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative initiation.

Protein type: Motility/polarity/chemotaxis; Activator

Cellular Component: extracellular space; cytoplasm; cytosol; nucleus

Molecular Function: heparin binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; growth factor activity; cytokine activity; chemoattractant activity; fibroblast growth factor receptor binding

Biological Process: wound healing; activation of MAPKK activity; regulation of cell cycle; positive regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; adrenocorticotropin hormone secreting cell differentiation; thyroid stimulating hormone secreting cell differentiation; hyaluronan catabolic process; growth factor dependent regulation of satellite cell proliferation; negative regulation of cell proliferation; embryonic development ending in birth or egg hatching; positive regulation of MAP kinase activity; glial cell differentiation; substantia nigra development; positive chemotaxis; induction of an organ; positive regulation of cell proliferation; regulation of retinal cell programmed cell death; angiogenesis; response to axon injury; aging; positive regulation of cardiac muscle cell proliferation; positive regulation of granule cell precursor proliferation; fibroblast growth factor receptor signaling pathway; positive regulation of cell fate specification; positive regulation of blood vessel endothelial cell migration; positive regulation of phosphoinositide 3-kinase activity; negative regulation of blood vessel endothelial cell migration; positive regulation of protein kinase B signaling cascade; positive regulation of osteoblast differentiation; stem cell development; positive regulation of angiogenesis; cell migration during sprouting angiogenesis; ureteric bud branching; release of sequestered calcium ion into cytosol; positive regulation of cell division; phosphatidylinositol biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; positive regulation of cell differentiation; inositol phosphate biosynthetic process; positive regulation of epithelial cell proliferation; lung development
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Research Articles on rFGF 2

1. bFGF-induced differentiation of dorsal root ganglia stem cells toward Schwann cells might be mediated by binding to fibroblast growth factor receptor-1 (FGFR-1) through activation of MAPK/ERK signal pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with rFGF 2 active protein

 Products by Pathway  Pathway Diagram
 Activated Point Mutants Of FGFR2 Pathway antibodies  Activated Point Mutants Of FGFR2 Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram

Diseases associated with rFGF 2 active protein

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >983 publications with rFGF 2 and Nervous System Diseases
 Necrosis Antibodies  >618 publications with rFGF 2 and Necrosis
 Wounds and Injuries Antibodies  >576 publications with rFGF 2 and Wounds and Injuries
 Inflammation Antibodies  >444 publications with rFGF 2 and Inflammation
 Heart Diseases Antibodies  >419 publications with rFGF 2 and Heart Diseases
 Myocardial Ischemia Antibodies  >347 publications with rFGF 2 and Myocardial Ischemia
 Disease Progression Antibodies  >340 publications with rFGF 2 and Disease Progression
 Lung Diseases Antibodies  >325 publications with rFGF 2 and Lung Diseases
 Hyperplasia Antibodies  >258 publications with rFGF 2 and Hyperplasia
 Lung Neoplasms Antibodies  >226 publications with rFGF 2 and Lung Neoplasms
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