• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Filter by


Clonality
Isotype
Type
Reactivity
Gene Name
View more Gene Name filters
Host
Application
Clone

Buy Ripa Lysis Buffer Buffer for Sale Online

Buy high-quality Ripa Lysis Buffer Buffer online. Research-grade Ripa Lysis Buffer Buffer available for metabolic research studies. Fast shipping, competitive prices.
Showing 1751 to 1800 of 4937 products
Mesothelin

Human Mesothelin HEK293 Overexpression Lysate

(#MBS8115043)

Gene Names:
MSLN; MPF; SMRP
Other Names:
Human MSLN/Mesothelin HEK293 Cell Lysate (WB positive control); mesothelin; Human Mesothelin Overexpression Lysate; Human MPF Overexpression Lysate; Human SMRP Overexpression Lysate; mesothelin isofor...
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
MMP-2

Mouse MMP-2 HEK293 Overexpression Lysate

(#MBS8117159)

Gene Names:
MMP2; GelA; Clg4a; MMP-2
Other Names:
Mouse MMP-2/MMP2 HEK293 Cell Lysate (WB positive control); matrix metallopeptidase 2; Mouse Clg4a Overexpression Lysate; Mouse GelA Overexpression Lysate; Mouse MMP-2 Overexpression Lysate; 72 kDa typ...
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
LAG3

Human LAG3 HEK293 Overexpression Lysate

(#MBS8115683)

Gene Names:
LAG3; CD223
Other Names:
Human LAG3/CD223/Lymphocyte activation gene 3 HEK293 Cell Lysate (WB positive control); lymphocyte-activation gene 3; Human CD223 Overexpression Lysate; Human LAG-3 Overexpression Lysate; lymphocyte a...
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
IL7R alpha/CD127

Human IL7R alpha/CD127 HEK293 Overexpression Lysate

(#MBS8114267)

Gene Names:
IL7R
Other Names:
Human CD127/IL-7RA CHO Cell Lysate (WB positive control); interleukin 7 receptor; Human CD127 Overexpression Lysate; Human CDW127 Overexpression Lysate; Human IL-7R Overexpression Lysate; Human IL-7R-...
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
Glypican 3

Human Glypican 3 HEK293 Overexpression Lysate

(#MBS8113449)

Gene Names:
GPC3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
Other Names:
Human Glypican 3/GPC3/OCI-5 HEK293 Cell Lysate (WB positive control); glypican 3; Human DGSX Overexpression Lysate; Human Glypican 3 Overexpression Lysate; Human GTR2-2 Overexpression Lysate; Human MX...
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
DDR1

Rat DDR1 HEK293 Overexpression Lysate

(#MBS8117641)

Gene Names:
DDR1; Cak; Drd1; PTK3D
Other Names:
Rat DDR1 Kinase/MCK10/CD167 HEK293 Cell Lysate (WB positive control); discoidin domain receptor tyrosine kinase 1; Rat Cak Overexpression Lysate; Rat Drd1 Overexpression Lysate; Rat PTK3D Overexpressi...
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
DDR1

Mouse DDR1 HEK293 Overexpression Lysate

(#MBS8116900)

Gene Names:
DDR1; Cak; Nep; PTK3A; CD167a; AI323681; 6030432F18
Other Names:
Mouse DDR1 Kinase/MCK10/CD167 HEK293 Cell Lysate (WB positive control); discoidin domain receptor tyrosine kinase 1; Mouse 6030432F18 Overexpression Lysate; Mouse AI323681 Overexpression Lysate; Mouse...
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
Cadherin 8

Human Cadherin 8 HEK293 Overexpression Lysate

(#MBS8113506)

Gene Names:
CDH8; Nbla04261
Other Names:
Human Cadherin-8/CDH8 HEK293 Cell Lysate (WB positive control); cadherin 8; type 2; Human Nbla04261 Overexpression Lysate; cadherin-8 preproprotein
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
CDNF

Human CDNF HEK293 Overexpression Lysate

(#MBS8115553)

Gene Names:
CDNF; ARMETL1
Other Names:
Human CDNF/ARMETL1 HEK293 Cell Lysate (WB positive control); cerebral dopamine neurotrophic factor; Human ARMETL1 Overexpression Lysate
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
CD81

Rat CD81 HEK293 Overexpression Lysate

(#MBS8117564)

Gene Names:
CD81; Tapa1
Other Names:
Rat CD81/TAPA-1 HEK293 Cell Lysate (WB positive control); CD81 molecule; CD81 antigen
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
CD48

Human CD48 HEK293 Overexpression Lysate

(#MBS8114140)

Gene Names:
CD48; BCM1; BLAST; hCD48; mCD48; BLAST1; SLAMF2; MEM-102
Other Names:
Human CD48/SLAMF2/BCM1 HEK293 Cell Lysate (WB positive control); CD48 molecule; Human BCM1 Overexpression Lysate; Human BLAST Overexpression Lysate; Human Blast-1 Overexpression Lysate; Human BLAST1 O...
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
CD44

Human CD44 HEK293 Overexpression Lysate

(#MBS8114924)

Gene Names:
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Other Names:
Human CD44 HEK293 Cell Lysate (WB positive control); CD44 molecule (Indian blood group); Human CDW44 Overexpression Lysate; Human CSPG8 Overexpression Lysate; Human ECMR-III Overexpression Lysate; Hum...
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
CD24

Rat CD24 HEK293 Overexpression Lysate

(#MBS8117531)

Gene Names:
CD24; Cd24a
Other Names:
Rat CD24/Ly-52/CD24A HEK293 Cell Lysate (WB positive control); CD24 molecule; Rat CD24 Overexpression Lysate; Rat Cd24a Overexpression Lysate; signal transducer CD24
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
CD1E & B2M Heterodimer

Cynomolgus CD1E & B2M Heterodimer HEK293 Overexpression Lysate

(#MBS8118094)

Gene Names:
CD1E; B2M
Other Names:
Cynomolgus CD1E & B2M Heterodimer HEK293 Cell Lysate (WB positive control); Cynomolgus CD1E & B2M Heterodimer Overexpression Lysate
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
AKT3

Mouse AKT3 Baculovirus-Insect cells Overexpression Lysate

(#MBS8116999)

Gene Names:
AKT3; Nmf350; AI851531; D930002M15Rik
Other Names:
Mouse AKT3 (aa 106-479) Insect Cell Lysate (WB positive control); v-akt murine thymoma viral oncogene homolog 3; Mouse AI851531 Overexpression Lysate; Mouse D930002M15Rik Overexpression Lysate; Mouse ...
Host/Reactivities:
Host: Baculovirus-Insect cells
Applications:
Western Blot (WB)
ACP5

Human ACP5 HEK293 Overexpression Lysate

(#MBS8113942)

Gene Names:
ACP5; TRAP; SPENCDI
Other Names:
Human ACP5/TRAP HEK293 Cell Lysate (WB positive control); acid phosphatase 5; tartrate resistant; Human SPENCDI Overexpression Lysate; Human TRAP Overexpression Lysate; tartrate-resistant acid phospha...
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
Thrombomodulin

Rat Thrombomodulin HEK293 Overexpression Lysate

(#MBS8117583)

Gene Names:
THBD
Other Names:
Rat Thrombomodulin/THBD HEK293 Cell Lysate (WB positive control); thrombomodulin
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
Syntaxin 8

Human Syntaxin 8 HEK293 Overexpression Lysate

(#MBS8115356)

Gene Names:
STX8; CARB
Other Names:
Human STX8/Syntaxin 8 HEK293 Cell Lysate (WB positive control); syntaxin 8; Human CARB Overexpression Lysate
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
PLK1

Mouse PLK1 Baculovirus-Insect cells Overexpression Lysate

(#MBS8116743)

Gene Names:
PLK1; Plk; STPK13
Other Names:
Mouse PLK1/PLK-1 Insect Cell Lysate (WB positive control); polo-like kinase 1; Mouse Plk Overexpression Lysate; Mouse STPK13 Overexpression Lysate; serine/threonine-protein kinase PLK1
Host/Reactivities:
Host: Baculovirus-Insect cells
Applications:
Western Blot (WB)
Western Blot (WB)

Rabbit anti-PIT2 Antibody, Affinity Purified

(#MBS3904651)

Gene Names:
PIT2; SLC20A2; RAM1; GLVR2; IBGC1; IBGC3; MLVAR; PIT-2; GLVR-2
Other Names:
Ram-1; Solute carrier family 20 member 2; sodium-dependent phosphate transporter 2; solute carrier family 20 (phosphate transporter); member 2; Phosphate transporter 2; receptor; murine leukemia virus...
Host/Reactivities:
Host: Rabbit / Reactivity: Human, Mouse
Purification:
Antigen Affinity Purified
Applications:
Western Blot (WB)
HRAS

Human HRAS Baculovirus-Insect cells Overexpression Lysate

(#MBS8114877)

Gene Names:
HRAS; CTLO; HAMSV; HRAS1; K-RAS; N-RAS; RASH1; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1
Other Names:
Human HRAS/GTPase Hras Insect Cell Lysate (WB positive control); Harvey rat sarcoma viral oncogene homolog; Human C-BAS/HAS Overexpression Lysate; Human C-H-RAS Overexpression Lysate; Human C-HA-RAS1 ...
Host/Reactivities:
Host: Baculovirus-Insect cells
Applications:
Western Blot (WB)
GGT1

Rat GGT1 HEK293 Overexpression Lysate

(#MBS8117595)

Gene Names:
GGT1; Ggt; Ggtp; GGLUT
Other Names:
Rat GGT1 HEK293 Cell Lysate (WB positive control); gamma-glutamyltransferase 1; gamma-glutamyltranspeptidase 1
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
FUT8

Human FUT8 Baculovirus-Insect cells Overexpression Lysate

(#MBS8114524)

Gene Names:
FUT8
Other Names:
Human FUT8 (aa 68-575) Insect Cell Lysate (WB positive control); fucosyltransferase 8 (alpha (1; 6) fucosyltransferase); Human MGC26465 Overexpression Lysate; Alpha-(1,6)-fucosyltransferase; fucosyltr...
Host/Reactivities:
Host: Baculovirus-Insect cells
Applications:
Western Blot (WB)
FGL1

Human FGL1 Baculovirus-Insect Overexpression Lysate

(#MBS8115173)

Gene Names:
FGL1; HFREP1; HP-041; LFIRE1; LFIRE-1
Other Names:
Human FGL1 Insect Cell Lysate (WB positive control); fibrinogen-like 1; Human HFREP1 Overexpression Lysate; Human HP-041 Overexpression Lysate; Human LFIRE-1 Overexpression Lysate; Human LFIRE1 Overex...
Host/Reactivities:
Host: Baculovirus-Insect Cells
Applications:
Western Blot (WB)
Cystatin S

Human Cystatin S HEK293 Overexpression Lysate

(#MBS8114569)

Gene Names:
CST4
Other Names:
Human Cystatin S/CST4 HEK293 Cell Lysate (WB positive control); cystatin S; Human CST4 Overexpression Lysate; Human MGC71923 Overexpression Lysate; cystatin-S
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
Cathepsin L

Human Cathepsin L HEK293 Overexpression Lysate

(#MBS8113898)

Gene Names:
CTSL; CTSL1; MEP; CATL; FLJ31037
Other Names:
Human Cathepsin L1/CTSL1 HEK293 Cell Lysate (WB positive control); cathepsin L; Human CATL Overexpression Lysate; Human CTSL Overexpression Lysate; Human CTSL1 Overexpression Lysate; Human FLJ31037 Ov...
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
Calreticulin

Human Calreticulin HEK293 Overexpression Lysate

(#MBS8115196)

Gene Names:
CALR; RO; CRT; SSA; cC1qR
Other Names:
Human CALR/Calreticulin HEK293 Cell Lysate (WB positive control); calreticulin; Human cC1qR Overexpression Lysate; Human CRT Overexpression Lysate; Human HEL-S-99n Overexpression Lysate; Human RO Over...
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
CD96

Human CD96 HEK293 Overexpression Lysate

(#MBS8114470)

Gene Names:
CD96; TACTILE
Other Names:
Human CD96 HEK293 Cell Lysate (WB positive control); CD96 molecule; Human TACTILE Overexpression Lysate; T-cell surface protein tactile
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
CD58

Cynomolgus CD58 HEK293 Overexpression Lysate

(#MBS8117885)

Gene Names:
CD58
Other Names:
Cynomolgus LFA-3/CD58 HEK293 Cell Lysate (WB positive control); CD58 molecule; Cynomolgus CD58 Overexpression Lysate; Cynomolgus LFA3 Overexpression Lysate
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
CD52

Rat CD52 HEK293 Overexpression Lysate

(#MBS8117532)

Gene Names:
CD52; B7
Other Names:
Rat CD52/CDW52 HEK293 Cell Lysate (WB positive control); CD52 molecule; CAMPATH-1 antigen
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
CD40

Rhesus CD40 HEK293 Overexpression Lysate

(#MBS8117806)

Gene Names:
CD40
Other Names:
Rhesus CD40/TNFRSF5 HEK293 Cell Lysate (WB positive control); CD40 molecule; TNF receptor superfamily member 5
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
CD3E & CD3G Heterodimer

Mouse CD3E & CD3G Heterodimer HEK293 Overexpression Lysate

(#MBS8118108)

Gene Names:
CD3E; CD3G; CD3; T3e; AI504783; CD3epsilon
Other Names:
Mouse CD3E & CD3G Heterodimer HEK293 Cell Lysate (WB positive control); CD3e molecule; epsilon (CD3-TCR complex); Mouse AI504783 Overexpression Lysate; Mouse CD3 Overexpression Lysate; Mouse CD3epsilo...
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
BCAM

Rat BCAM HEK293 Overexpression Lysate

(#MBS8117475)

Gene Names:
BCAM; Lu
Other Names:
Rat BCAM HEK293 Cell Lysate (WB positive control); basal cell adhesion molecule (Lutheran blood group); basal cell adhesion molecule
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
ATP citrate lyase/ACLY

Human ATP citrate lyase/ACLY Baculovirus-Insect cells Overexpression Lysate

(#MBS8114770)

Gene Names:
ACLY; ACL; ATPCL; CLATP
Other Names:
Human ACLY/acly/ATP citrate lyase Insect Cell Lysate (WB positive control); ATP citrate lyase; Human ACL Overexpression Lysate; Human ATPCL Overexpression Lysate; Human CLATP Overexpression Lysate; AT...
Host/Reactivities:
Host: Baculovirus-Insect cells
Applications:
Western Blot (WB)
ART4/CD297

Mouse ART4/CD297 HEK293 Overexpression Lysate

(#MBS8117033)

Gene Names:
ART4; DO; DOK1; ARTC4; 4432404K01Rik
Other Names:
Mouse Art4/CD297 HEK293 Cell Lysate (WB positive control); ADP-ribosyltransferase 4 (Dombrock blood group); Mouse 4432404K01Rik Overexpression Lysate; Mouse ARTC4 Overexpression Lysate; Mouse DO Overe...
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
ART4/CD297

Cynomolgus ART4/CD297 HEK293 Overexpression Lysate

(#MBS8117947)

Gene Names:
ART4
Other Names:
Cynomolgus ART4 HEK293 Cell Lysate (WB positive control); ADP-ribosyltransferase 4 (Dombrock blood group)
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
carboxylesterase 2/CES2

Human carboxylesterase 2/CES2 HEK293 Overexpression Lysate

(#MBS8113790)

Gene Names:
CES2; iCE; CE-2; PCE-2; CES2A1
Other Names:
Human CES2/Carboxylesterase-2 HEK293 Cell Lysate (WB positive control); carboxylesterase 2; Human CE-2 Overexpression Lysate; Human CES2A1 Overexpression Lysate; Human iCE Overexpression Lysate; Human...
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
VRK1

Human VRK1 Baculovirus-Insect cells Overexpression Lysate

(#MBS8114576)

Gene Names:
VRK1; PCH1; PCH1A
Other Names:
Human VRK1 Insect Cell Lysate (WB positive control); vaccinia related kinase 1; Human PCH1 Overexpression Lysate; Human PCH1A Overexpression Lysate; VRK serine/threonine kinase 1
Host/Reactivities:
Host: Baculovirus-Insect cells
Applications:
Western Blot (WB)
USP5

Human USP5 Baculovirus-Insect cells Overexpression Lysate

(#MBS8114989)

Gene Names:
USP5; ISOT
Other Names:
Human USP5/ISOT Insect Cell Lysate (WB positive control); ubiquitin specific peptidase 5 (isopeptidase T); Human ISOT Overexpression Lysate; Ubiquitin carboxyl-terminal hydrolase 5
Host/Reactivities:
Host: Baculovirus-Insect cells
Applications:
Western Blot (WB)
PIGR

Human PIGR HEK293 Overexpression Lysate

(#MBS8113498)

Gene Names:
PIGR
Other Names:
Human PIGR (365 Ser/Gly) HEK293 Cell Lysate (WB positive control); polymeric immunoglobulin receptor; Human FLJ22667 Overexpression Lysate; Human MGC125361 Overexpression Lysate; Human MGC125362 Overe...
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
NGFR

Rabbit NGFR HEK293 Overexpression Lysate

(#MBS8117214)

Gene Names:
NGFR
Other Names:
Rabbit NGFR/TNFRSF16/P75 HEK293 Cell Lysate (WB positive control); nerve growth factor receptor (TNFR superfamily; member 16); Rabbit NGFR Overexpression Lysate
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
Leptin Receptor

Human Leptin Receptor HEK293 Overexpression Lysate

(#MBS8113704)

Gene Names:
LEPR; OBR; OB-R; CD295; LEP-R; LEPRD
Other Names:
Human Leptin Receptor/LEPR/CD295 HEK293 Cell Lysate (WB positive control); leptin receptor; Human CD295 Overexpression Lysate; Human LEP-R Overexpression Lysate; Human LEPRD Overexpression Lysate; Hum...
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
LTBR

Rat LTBR HEK293 Overexpression Lysate

(#MBS8117434)

Gene Names:
LTBR
Other Names:
Rat LTBR/TNFRSF3 HEK293 Cell Lysate (WB positive control); lymphotoxin beta receptor (TNFR superfamily; member 3); tumor necrosis factor receptor superfamily member 3; lymphotoxin beta receptor
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
LAG3

Mouse LAG3 HEK293 Overexpression Lysate

(#MBS8117162)

Gene Names:
LAG3; Ly66; CD223; LAG-3
Other Names:
Mouse LAG3/CD223/Lymphocyte activation gene 3 HEK293 Cell Lysate (WB positive control); lymphocyte-activation gene 3; Mouse CD223 Overexpression Lysate; Mouse LAG-3 Overexpression Lysate; Mouse Ly66 O...
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
E-Selectin

Rat E-Selectin HEK293 Overexpression Lysate

(#MBS8117349)

Gene Names:
SELE
Other Names:
Rat E-Selectin/CD62e/SELE HEK293 Cell Lysate (WB positive control); selectin E; E-selectin
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
E-Selectin

Cynomolgus E-Selectin HEK293 Overexpression Lysate

(#MBS8117871)

Gene Names:
SELE
Other Names:
Cynomolgus E-Selectin/CD62e/SELE HEK293 Cell Lysate (WB positive control); selectin E
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
DKK1

Rat DKK1 HEK293 Overexpression Lysate

(#MBS8117718)

Gene Names:
DKK1
Other Names:
Rat DKK1 CHO Cell Lysate (WB positive control); dickkopf WNT signaling pathway inhibitor 1; dickkopf-related protein 1
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
DDR1

Mouse DDR1 HEK293 Overexpression Lysate

(#MBS8116899)

Gene Names:
DDR1; Cak; Nep; PTK3A; CD167a; AI323681; 6030432F18
Other Names:
Mouse DDR1 Kinase/MCK10/CD167 HEK293 Cell Lysate (WB positive control); discoidin domain receptor tyrosine kinase 1; Mouse 6030432F18 Overexpression Lysate; Mouse AI323681 Overexpression Lysate; Mouse...
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
Cathepsin S

Human Cathepsin S HEK293 Overexpression Lysate

(#MBS8113899)

Gene Names:
CTSS
Other Names:
Human Cathepsin S/CTSS HEK293 Cell Lysate (WB positive control); cathepsin S; Human CTSS Overexpression Lysate; Human MGC3886 Overexpression Lysate; cathepsin S isoform 1 preproprotein
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
Cathepsin L

Mouse Cathepsin L HEK293 Overexpression Lysate

(#MBS8116289)

Gene Names:
CTSL; fs; MEP; nkt; CatL; Ctsl1; 1190035F06Rik
Other Names:
Mouse Cathepsin L/CTSL HEK293 Cell Lysate (WB positive control); cathepsin L; Mouse 1190035F06Rik Overexpression Lysate; Mouse Ctsl1 Overexpression Lysate; Mouse fs Overexpression Lysate; Mouse MEP Ov...
Host/Reactivities:
Host: HEK293 Cells
Applications:
Western Blot (WB)
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000