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SDF-1 Alpha (SDF-1alpha) Mouse Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS554234

MBS554234 is an active protein targeting SDF-1alpha. Also known as Mouse SDF-1 alpha Recombinant. Host: E coli (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.). More details

$240 / 0.002 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SDF-1 alpha (SDF-1alpha), Active Protein
Full Product Name
Mouse SDF-1 alpha Recombinant
Product Gene Name
SDF-1alpha active protein
Sequence
MDAKVVAVLA LVLAALCISD GKPVSLSYRC PCRFFESHIA RANVKHLKIL NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALNK
Host
E coli (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
Form/Format
Recombinant SDF-1 alpha was lyophilized from a 0.2 um filtered solution in.5% glycine,.5% sucrose,.01% Tween80, mM Glutamic acid, pH.5.
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Preparation and Storage
The lyophilized protein is stable for at least years from date of receipt at-20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Molecular Weight
10,032 Da
3D Structure
ModBase 3D Structure for P40224
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Source
Optimized DNA sequence encoding Mouse SDF-1 alpha mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Biological Activity
Determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentration range of-40 ng/ml.
Method
Cell MigrationThe upper chamber wells of CIM-Plates 16 were precoated with fibronectin, added to wells at 10 ug/ml (30 ul) and removed after 30 min.
The lower chamber wells were filled with 160 ul culture medium, allowing a clearly-defined meniscus to form.
SDF1, MCP1 and TNFalpha were added to the lower chambers at a final concentration of 100 ng/ml.
CIM-Plates were equilibrated for at least 30 min in a culture hood at room temperature.
Trypsin-EDTA-dispersed MPCs (100 ul in complete medium) were then plated in the fibronectin-precoated upper chamber wells at 306×103
cells/cm2 (600000 cells/ml) in 100 ul complete medium, which we determined as the optimal density to ensure confluent monolayers.The CIM-Plates were inserted into the cradle pockets of the RTCA DP
Analyzer, and the background impedance of each well was measured after 1 h. The RTCA DP Analyzer, placed in a standard cell-culture incubator, monitored the migrated cell number in each lower chamber well every 15 min (quadruplicate measurements) over 36 h. Data were analyzed with the RTCA Software.
Molecular Function
Cytokine, Growth-factor
Biological Process
Chemotaxis
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Product Categories/Family for SDF-1alpha active protein

Recombinant Mouse Cytokines; CXC chemokines

Testing Data of SDF-1alpha active protein

WHIM mutation CXCR4R334X increases CXCL12-induced calcium flux response, but allows homologous desensitization: analysis of transfected cells.
Mouse SDF-1alpha Active Protein – Cat# MBS554234

Testing Data of SDF-1alpha active protein

The expression of the ?lobe A and 3A mutant fails to restore motility and polarity in BW5147?–?– cells.
BW5147?–?– cells stably expressing WT or mutant DOCK2 (V1538A, ?lobe A, or 3A) were stimulated in suspension with CXCL12, and stained with phalloidin and DAPI.
Mouse SDF-1alpha Active Protein, Testing Data – Cat# MBS554234

Testing Data of SDF-1alpha active protein

Expression and function of the CXCR4 receptor in MET-depleted cell.
(b) Western blot analysis of AKT and MAPK activation in shMET RH30 cells showed a decrease in the phosphorylation of these kinases following stimulation with human HGF (20?ng/ml) and SDF-1 (10?ng/ml and 100?ng/ml).
Mouse SDF-1alpha Active Protein, Testing Data – Cat# MBS554234

Other Notes

Small volumes of SDF-1alpha active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SDF-1alpha. It may not necessarily be applicable to this product.

NCBI GI #
7305465
NCBI GeneID
20315
NCBI Accession #
NP_038683.1
UniProt Primary Accession #
P40224
UniProt Secondary Accession #
Q8JLJ0; Q8QMN0; Q9JFT2
UniProt Related Accession #
P40224
GenBank Nucleotide #
NM_013655.4
NCBI Official Full Name
stromal cell-derived factor 1 isoform beta
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 12
NCBI Official Symbol
Cxcl12
NCBI Official Synonym Symbols
Pbsf; Sdf1; Tlsf; Tpar1; Scyb12
UniProt Protein Name
Stromal cell-derived factor 1
UniProt Synonym Protein Names
12-O-tetradecanoylphorbol 13-acetate repressed protein 1; TPAR1; C-X-C motif chemokine 12; Pre-B cell growth-stimulating factor; PBSF; Thymic lymphoma cell-stimulating factor; TLSF
UniProt Gene Name
Cxcl12
UniProt Synonym Gene Names
Sdf1; SDF-1; TPAR1; PBSF; TLSF
UniProt Entry Name
SDF1_MOUSE
NCBI Protein Information
stromal cell-derived factor 1

UniProt Comments for SDF-1alpha

Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells (). Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SDF-1alpha active protein

 Reference Product  PubMed Publications
 Cxcr4 active protein  >3939 publications with SDF-1alpha and Cxcr4
 Ccr5 active protein  >315 publications with SDF-1alpha and Ccr5
 Ccr2 active protein  >182 publications with SDF-1alpha and Ccr2
 Ccr7 active protein  >173 publications with SDF-1alpha and Ccr7
 Cxcr3 active protein  >140 publications with SDF-1alpha and Cxcr3
 Ccr1 active protein  >70 publications with SDF-1alpha and Ccr1
 Ccr4 active protein  >44 publications with SDF-1alpha and Ccr4
 Ccr3 active protein  >40 publications with SDF-1alpha and Ccr3

Pathways associated with SDF-1alpha active protein

 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram

Diseases associated with SDF-1alpha active protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >661 publications with SDF-1alpha and Inflammation
 Cardiovascular Diseases Antibodies  >645 publications with SDF-1alpha and Cardiovascular Diseases
 Neoplasm Metastasis Antibodies  >456 publications with SDF-1alpha and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >302 publications with SDF-1alpha and Breast Neoplasms
 Heart Diseases Antibodies  >296 publications with SDF-1alpha and Heart Diseases
 Ischemia Antibodies  >276 publications with SDF-1alpha and Ischemia
 Neoplasm Invasiveness Antibodies  >276 publications with SDF-1alpha and Neoplasm Invasiveness
 HIV Infections Antibodies  >270 publications with SDF-1alpha and HIV Infections
 Lung Diseases Antibodies  >256 publications with SDF-1alpha and Lung Diseases
 Necrosis Antibodies  >253 publications with SDF-1alpha and Necrosis

Organs/Tissues associated with SDF-1alpha active protein

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >1701 publications with SDF-1alpha and Bone
 Bone Marrow Antibodies  >1534 publications with SDF-1alpha and Bone Marrow
 Brain Antibodies  >572 publications with SDF-1alpha and Brain
 Muscle Antibodies  >411 publications with SDF-1alpha and Muscle
 Lung Antibodies  >387 publications with SDF-1alpha and Lung
 Heart Antibodies  >352 publications with SDF-1alpha and Heart
 Lymph Node Antibodies  >326 publications with SDF-1alpha and Lymph Node
 Liver Antibodies  >317 publications with SDF-1alpha and Liver
 Embryonic Tissue Antibodies  >236 publications with SDF-1alpha and Embryonic Tissue
 Skin Antibodies  >173 publications with SDF-1alpha and Skin
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