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FCR1 Small Molecule

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS807938

FCR1 or flavin coumarin redox sensor 1, is a novel ratiometric fluorescent redox sensor utilized in fluorescence lifetime imaging microscopy and flow cytometry. In the oxidized form, excitation of FCR1 at 405nm results in a green fluorescence with max… More details

$680 / 1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
FCR1, Small Molecule
Full Product Name
FCR1
Product Gene Name
Product Synonym Names
FRET ratiometric fluorescence-based redox sensor; 7-(Diethylamino)-N-((1r,4r)-4-(2-(10-ethyl-2,4-dioxo-4,10-dihydrobenzo[g]pteridin-3(2H)-yl)acetamido)cyclohexyl)-2-oxo-2H-chromene-3-carboxamide (FCR1)
Host
Synthetic
Form/Format
Orange solid
Preparation and Storage
Store at -20 degree C.
Molecular Weight
639
Solubility
Soluble in DMSO
Molecular Formula
C34H37N7O6

Chemical Structure of FCR1 small molecule

Chemical structure and design of FCR1 (SIH-180), showing FRET processes in oxidised form. Inset: photographs of cuvettes of FCR1 in oxidised and reduced forms under 365 nm excitation. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.
FCR1 Small Molecule – Cat# MBS807938

Immunofluorescence (IF) of FCR1 small molecule

Fluorescence behavior of FCR1 (SIH-180) in the oxidized (green) and reduced (blue) forms, using 10 uM. Excitation: 405 nm. Reduced Emission: 475 nm. Oxidized Emission: 520 nm. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.
FCR1 Small Molecule, Immunofluorescence (IF) – Cat# MBS807938

Testing Data of FCR1 small molecule

Two photon - confocal microscopy imaging of HeLa cells treated with FCR1 (SIH-180, 10 uM, 15 min, ex = 820 nm) and (a) N-acetyl cysteine (50 uM, 30 min), (b) vehicle control and (c) H2O2 (50 uM, 30 min) in blue and green channels. The pseudo colour ratio images indicate the ratio of emission intensity in the green channel to blue channel. Scale bar represents 20 um. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.
FCR1 Small Molecule, Testing Data – Cat# MBS807938

Immunofluorescence (IF) of FCR1 small molecule

Fluorescence lifetimes of the donor (420 - 470 nm) in HeLa cells treated with FCR1 (SIH-180, 10 uM, ex = 820 nm) and N-acetyl cysteine, vehicle control and H2O2. Pseudo-colour images represent mean lifetime. Scale bar represents 50 um. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.
FCR1 Small Molecule, Immunofluorescence (IF) – Cat# MBS807938

Flow Cytometry (FC/FACS) of FCR1 small molecule

Flow cytometric studies of HeLa cells treated with FCR1 (SIH-180, 10 uM, ex = 405 nm) showing the fluorescence ratio (560 / 450 nm) when treated with N-acetyl cysteine (blue), vehicle control (green) and H2O2 (red). Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.
FCR1 Small Molecule, Flow Cytometry (FC/FACS) – Cat# MBS807938

Product References and Citations for FCR1 small molecule

1. Kaur A., Haghighatbin M.A., Hogan C.F., and New E.J. (2015) Chem. Commun. Epub.

Other Notes

Small volumes of FCR1 small molecule vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FCR1 purchase

MBS807938 is a small molecule targeting FCR1. Host: Synthetic.

Additional Details

NCBI/Uniprot data below describe general gene information for FCR1. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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