SPN Cell Lysate – Tissue Lysate

CD43 is an abundantly expressed molecule on the T-cell surface that shows distinct localization to the migrating T-cell uropod and the distal pole complex (DPC) opposite the immunological synapse via association with the ezrin-radixin-moesin (ERM) family of actin regulatory proteins. CD43 has a 235-amino acid (aa) extracellular domain, a 23-aa transmembrane domain, and a 123-aa cytoplasmic domain, all encoded by a single exon. The intracytoplasmic region of the protein is necessary to transduce signals; it is rich in potentially phosphorylable threonines and serines but lacks tyrosine residues as well as catalytic activity. CD43 engagement on human peripheral blood T cells and monocytes leads to cell activation and proliferation through the generation of second messengers such as diacylglycerol and inositol phosphates, protein kinase C (PKC) activation and Ca2+ mobilization. Besides, CD43 ligation on human T cells induces the association of CD43 with Src family kinases, presumably through the interaction of their Src homology 3 domain with a proline-rich region of the CD43 intracytoplasmic tail. This molecule has been implicated in T cell activation, enhancing T cell response to allogeneic or mitogenic stimulation and CD43-specific signals have been reported to be sufficient to activate T cells in the absence of T cell receptor (TCR) engagement. In summary, CD43 regulates multiple T-cell functions, including T-cell activation, proliferation, apoptosis, and migration.

This Mouse CD43 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD43 protein from the overexpression lysate was verified.

Layseca-Espinosa E, et al. (2003) Journal of Leukocyte Biology. 74(6): 1083-93. Cannon JL, et al. (2011) Mol Biol Cell. 22(7):954-63. Pallant A, et al. (1989). Proc Natl Acad Sci. 86 (4): 1328-32.

Small volumes of SPN cell lysate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS8116840 is a cell lysate targeting SPN. Also known as Mouse CD43 HEK293 Overexpression Lysate. Host: HEK293 Cells.