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Foxp3/Transcription Factor (FOXP3) Staining Kit

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS668928

The FoxP3 / Transcription Factor Staining Kit has optimized for staining with antibodies to transcription factors and nuclear proteins, such as FoxP3. This kit can be used for staining cytokines and chemokines. More details

$485 / 1 Kit +1 FREE 8GB USB

Product Documentation

PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Foxp3/Transcription Factor, Staining Kit
Full Product Name
Foxp3/Transcription Factor Staining Kit
Product Gene Name
FOXP3 staining kit
Product Synonym Names
Human Foxp3 Fixation/Permeabilization Kit Kits and Reagents; Kits and Reagents
Preparation and Storage
Store at 2-8 degree C
Molecular Weight
44,407 Da
OMIM
AF277993 mRNA

Product Categories/Family for FOXP3 staining kit

Kits and Reagents; Flowcytometry staining Kits
Applications Tested/Suitable for FOXP3 staining kit
Flow Cytometry (FC/FACS)
Application Notes for FOXP3 staining kit
Protocol: FoxP3 FITC staining with CD4 PE and CD25 APC antibodies. 1. Determine number of cells required for staining. Each sample contains 0.5 -1 x 10^6 cells in 50ul of media or staining buffer. The following controls are needed for the experiment. Unstained cells (no antibodies were added), cells with isotype control and cells with secondary antibody (if secondary antibody was used). 2. Centrifuge cells at 1000 RPM for 10 minutes and decant supernatant. 3. Resuspend cells with appropriate volume of staining buffer. 4. Aliquote 1x10^6 cells in 50 ul to the desired number of flow tubes. Dilute anti-CD4 PE, anti-CD25 APC cell surface antibodies in 50 ul of 1X staining buffer. Add diluted antibodies into 50 ul of cell suspension. Mix antibodies in cells suspension thoroughly. 5. Incubate in ice for 30 minutes in ice pritected from light. Wash cells in 2-3 ml of 1X staining buffer. Centrifuge 1000 RPM for 10 minutes. Decant supernatant carefully. 6. Add 1ml of freshly prepared 1X fixation/permeabilization buffer. Mix well. 7. Incubate in ice for 30 minutes protected from light. 8. Whsh cells in 3-4 ml of 1X permeabilization buffer. Centrifuge at 1000 RPM for 10 minutes. Decant supernatant carefully. 9. Add 2% mouse serum to the cells in 50 ul of 1X permeabilization buffer (for blocking non-specific binding). Incubate 10 minutes in ice. (Optional) 10. Add fluorochrome conjugated Anti-FoxP3 antibody or isotype control in 50 ul of 1X permeabilization buffer. (Without washing blocking step). 11. Incubate in ice for 30 minutes, protected from light. Wash cells 3-4 ml of 1X permeabilization buffer. Centrifuge 1000 RPM for 10 minutes. Decant supernatant carefully. 11. After decanting, add 300-400ul of staining buffer to each tube. If not analyzing same day, samples can be stored over night in dark at 4 degrees C. Samples can be analyzed in Flow Cytometer according to the manufacturer protocol.

Testing Data of FOXP3 staining kit

Human PBMC were stained with FoxP3/Transcription Factor staining kit (17-1014). Anti-CD4 PE and anti-CD25 APC positive cells were gated, further analyzed in anti-FoxP3 FITC using 2 ug antibodies. Green represents FITC conjugated Isotope control . Red represents anti-FoxP3 FITC.
FOXP3 Staining Kit – Cat# MBS668928

Other Notes

Small volumes of FOXP3 staining kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FOXP3 purchase

MBS668928 is a staining kit targeting FOXP3. Also known as Foxp3/Transcription Factor Staining Kit.

Additional Details

NCBI/Uniprot data below describe general gene information for FOXP3. It may not necessarily be applicable to this product.

NCBI GI #
146262391
NCBI GeneID
50943
NCBI Accession #
ABQ15210.1
UniProt Secondary Accession #
O60827; Q14DD8; Q4ZH51; A5HJT1; B7ZLG0; B9UN80
UniProt Related Accession #
Q9BZS1
NCBI Official Full Name
FOXP3
NCBI Official Synonym Full Names
forkhead box P3
NCBI Official Symbol
FOXP3
NCBI Official Synonym Symbols
JM2; AIID; IPEX; PIDX; XPID; DIETER
UniProt Protein Name
Forkhead box protein P3
UniProt Synonym Protein Names
Scurfin
Protein Family
Forkhead box protein
UniProt Gene Name
FOXP3
UniProt Synonym Gene Names
IPEX
NCBI Protein Information
forkhead box protein P3

NCBI Summary for FOXP3

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
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UniProt Comments for FOXP3

FOXP3: Probable transcription factor. Plays a critical role in the control of immune response. Interacts with IKZF3. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Cell cycle regulation; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: cytoplasm; nucleus

Molecular Function: histone acetyltransferase binding; histone deacetylase binding; NFAT protein binding; protein binding; protein homodimerization activity; sequence-specific DNA binding; transcription factor activity

Biological Process: CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment; inhibition of CREB transcription factor; inhibition of NF-kappaB transcription factor; negative regulation of cell proliferation; negative regulation of cytokine biosynthetic process; negative regulation of cytokine secretion; negative regulation of histone deacetylation; negative regulation of immune response; negative regulation of interferon-gamma production; negative regulation of interleukin-10 production; negative regulation of interleukin-17 production; negative regulation of interleukin-2 biosynthetic process; negative regulation of interleukin-2 production; negative regulation of interleukin-4 production; negative regulation of T cell cytokine production; negative regulation of T cell proliferation; negative regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of T cell anergy; response to virus; T cell activation

Disease: Diabetes Mellitus, Insulin-dependent; Immunodysregulation, Polyendocrinopathy, And Enteropathy, X-linked
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Research Articles on FOXP3

1. CD44 has a regulatory role in FOXP3 expression and is associated with good prognostic factors in breast cancer

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with FOXP3 staining kit

 Reference Product  PubMed Publications
 CD4 staining kit  >8646 publications with FOXP3 and CD4
 TNF staining kit  >819 publications with FOXP3 and TNF
 CTLA4 staining kit  >495 publications with FOXP3 and CTLA4
 TGFB1 staining kit  >466 publications with FOXP3 and TGFB1
 GATA3 staining kit  >238 publications with FOXP3 and GATA3
 IL2 staining kit  >122 publications with FOXP3 and IL2
 NFATC2 staining kit  >20 publications with FOXP3 and NFATC2
 STAT5A staining kit  >17 publications with FOXP3 and STAT5A
 CD8A staining kit  >10 publications with FOXP3 and CD8A

Pathways associated with FOXP3 staining kit

 Products by Pathway  Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 IL2 Signaling Events Mediated By STAT5 Pathway antibodies  IL2 Signaling Events Mediated By STAT5 Pathway Diagram
 Inflammatory Bowel Disease (IBD) Pathway antibodies  Inflammatory Bowel Disease (IBD) Pathway Diagram
 Inflammatory Bowel Disease (IBD) Pathway antibodies  Inflammatory Bowel Disease (IBD) Pathway Diagram

Diseases associated with FOXP3 staining kit

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2387 publications with FOXP3 and Inflammation
 Disease Models, Animal Antibodies  >1704 publications with FOXP3 and Disease Models, Animal
 Necrosis Antibodies  >799 publications with FOXP3 and Necrosis
 Nervous System Diseases Antibodies  >783 publications with FOXP3 and Nervous System Diseases
 Lung Diseases Antibodies  >697 publications with FOXP3 and Lung Diseases
 Liver Diseases Antibodies  >458 publications with FOXP3 and Liver Diseases
 Cardiovascular Diseases Antibodies  >409 publications with FOXP3 and Cardiovascular Diseases
 Encephalomyelitis, Autoimmune, Experimental Antibodies  >402 publications with FOXP3 and Encephalomyelitis, Autoimmune, Experimental
 Diabetes Mellitus, Type 1 Antibodies  >389 publications with FOXP3 and Diabetes Mellitus, Type 1
 Neoplasms, Experimental Antibodies  >324 publications with FOXP3 and Neoplasms, Experimental

Organs/Tissues associated with FOXP3 staining kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >10176 publications with FOXP3 and Blood
 Lymph Node Antibodies  >1213 publications with FOXP3 and Lymph Node
 Spleen Antibodies  >1189 publications with FOXP3 and Spleen
 Thymus Antibodies  >824 publications with FOXP3 and Thymus
 Lung Antibodies  >794 publications with FOXP3 and Lung
 Eye Antibodies  >134 publications with FOXP3 and Eye
 Ascites Antibodies  >19 publications with FOXP3 and Ascites
 Trachea Antibodies  >11 publications with FOXP3 and Trachea
 Mammary Gland Antibodies  >3 publications with FOXP3 and Mammary Gland
 Uncharacterized Tissue Antibodies  >1 publications with FOXP3 and Uncharacterized Tissue
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