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Vimentin (VIM) Antibody – Rabbit

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4381338

MBS4381338 is an antibody targeting VIM. Also known as Vimentin (Mesenchymal Cell Marker). Reactive in Human. Host: Rabbit. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Vimentin, Antibody
Full Product Name
Vimentin (Mesenchymal Cell Marker)
Product Gene Name
anti-VIM antibody
Product Synonym Names
VIM
Isotype
IgG
Clone Number
VIM/1937R
Host
Rabbit
Species Reactivity
Human
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
57-60kDa
OMIM
193060
3D Structure
ModBase 3D Structure for P08670
Positive Control
U-87, A375, Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.
Immunogen
Recombinant full-length human Vimentin protein
Cellular Localization
Cytoplasmic
Hu-chromosome Location
10p12.33
Antibody Type
Recombinant Rabbit Monoclonal Antibody
Applications Tested/Suitable for anti-VIM antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-VIM antibody
WB: 1-2ug/ml
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-VIM antibody

Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).
Rabbit Anti-VIM Antibody – Cat# MBS4381338

Immunohistochemistry (IHC) of anti-VIM antibody

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).
Rabbit Anti-VIM Antibody, Immunohistochemistry (IHC) – Cat# MBS4381338

Immunohistochemistry (IHC) of anti-VIM antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).
Rabbit Anti-VIM Antibody, Immunohistochemistry (IHC) – Cat# MBS4381338

SDS-Page of anti-VIM antibody

SDS-PAGE Analysis Purified Vimentin Mouse Monoclonal Antibody (VIM/1937R). Confirmation of Purity and Integrity of Antibody.
Rabbit Anti-VIM Antibody, SDS-Page – Cat# MBS4381338

Western Blot (WB) of anti-VIM antibody

Western Blot Analysis of human A375 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).
Rabbit Anti-VIM Antibody, Western Blot (WB) – Cat# MBS4381338

Western Blot (WB) of anti-VIM antibody

Western Blot Analysis of human U-87 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).
Rabbit Anti-VIM Antibody, Western Blot (WB) – Cat# MBS4381338

Product References and Citations for anti-VIM antibody

Osborn M et. al. European Journal of Cell Biology. 1984; 34:137-143

Other Notes

Small volumes of anti-VIM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for VIM. It may not necessarily be applicable to this product.

NCBI GI #
62414289
NCBI GeneID
7431
NCBI Accession #
NP_003371.2
UniProt Primary Accession #
P08670
UniProt Related Accession #
P08670
GenBank Nucleotide #
NP_003371.2
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
UniProt Protein Name
Vimentin
Protein Family
Vimentin
UniProt Gene Name
VIM
UniProt Entry Name
VIME_HUMAN
NCBI Protein Information
vimentin

NCBI Summary for VIM

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
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UniProt Comments for VIM

Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: intermediate filament cytoskeleton; neuron projection; focal adhesion; cytoskeleton; cytoplasm; leading edge; plasma membrane; intermediate filament; peroxisome; cytosol

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens

Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; cell structure disassembly during apoptosis; muscle filament sliding

Disease: Cataract 30
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Research Articles on VIM

1. Intestinal type of gastric cancer associated with connective tissue dysplasia is characterized by a high level of expression of galectin-1 and vimentin in the perifocal zone and TGF-beta in the tumor zone.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-VIM antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >383 publications with VIM and Nervous System Diseases
 Neoplasms Antibodies  >283 publications with VIM and Neoplasms
 Brain Diseases Antibodies  >246 publications with VIM and Brain Diseases
 Neurodegenerative Diseases Antibodies  >166 publications with VIM and Neurodegenerative Diseases
 Inflammation Antibodies  >46 publications with VIM and Inflammation
 Kidney Diseases Antibodies  >37 publications with VIM and Kidney Diseases
 Hemorrhage Antibodies  >32 publications with VIM and Hemorrhage
 Disease Models, Animal Antibodies  >32 publications with VIM and Disease Models, Animal
 Pain Antibodies  >31 publications with VIM and Pain
 Liver Diseases Antibodies  >29 publications with VIM and Liver Diseases

Organs/Tissues associated with anti-VIM antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >461 publications with VIM and Brain
 Blood Antibodies  >187 publications with VIM and Blood
 Muscle Antibodies  >101 publications with VIM and Muscle
 Nerve Antibodies  >79 publications with VIM and Nerve
 Bone Antibodies  >69 publications with VIM and Bone
 Kidney Antibodies  >52 publications with VIM and Kidney
 Liver Antibodies  >51 publications with VIM and Liver
 Bone Marrow Antibodies  >46 publications with VIM and Bone Marrow
 Eye Antibodies  >38 publications with VIM and Eye
 Vascular Antibodies  >35 publications with VIM and Vascular
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