Mouse mMIP 1b Active Protein – Cat# MBS143275

Macrophage Inflammatory Protein-1 Beta (mMIP 1b) Mouse Active Protein

Name: mMIP 1b Active Protein; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS143275
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS143275

Description: Macrophage Inflammatory Protein-1 beta Mouse Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7808 Dalton. The MIP-1b is purified by proprietary… More details

$250 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Macrophage Inflammatory protein-1 beta (mMIP 1b), Active Protein

Full Product Name

Recombinant Mouse Macrophage Inflammatory protein-1 beta (CCL4)

Product Gene Name

mMIP 1b active protein

Product Synonym Names

MIP 1b Mouse; Macrophage Inflammatory Protein-1 beta Mouse Recombinant (CCL4); Small inducible cytokine A4; CCL4; Macrophage inflammatory protein 1-beta; MIP-1- beta; MIP-1-beta(1-69); T-cell activation protein 2; ACT-2; PAT 744; H400; SIS-gamma; Lymphocyte activation gene 1 protein; LAG-1; HC21; G-26 T-lymphocyte-secreted protein; chemokine (C-C motif) ligand 4; ACT2; G-26; LAG1; MIP1B; SCYA2; SCYA4; AT744.1; MGC104418; MGC126025; MGC126026

Sequence

APYGADTPTA CCFSYSRKIP RQFIVDYFET SSLCSQPGVI FLTKRNRQIC ADSKETWVQE YITDLELNA

Host

E Coli

Form/Format

Lyophilized from a concentrated (1 mg/ml) solution in water containing no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity/Purification

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Preparation and Storage

Lyophilized Mouse MIP-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Mouse CCL4 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Molecular Weight

10,168 Da

3D Structure

ModBase 3D Structure for P14097

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility

It is recommended to reconstitute the lyophilized Mouse MIP-1b in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

Mouse MIP-1 beta activity is calculated by the ability to chemoattract Human blood monocytes at 20-100ng/ml corresponding to a Specific Activity of 10,000-50,000IU/mg.

Product Categories/Family for mMIP 1b active protein

Chemokines; MIP

Other Notes

Small volumes of mMIP 1b active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for mMIP 1b purchase

MBS143275 is an active protein targeting mMIP 1b. Also known as Recombinant Mouse Macrophage Inflammatory protein-1 beta (CCL4). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for mMIP 1b. It may not necessarily be applicable to this product.

NCBI GI #

7305459

NCBI GeneID

20303

NCBI Accession #

NP_038680.1

UniProt Primary Accession #

P14097

UniProt Related Accession #

P14097

GenBank Nucleotide #

NM_013652.2

NCBI Official Full Name

C-C motif chemokine 4

NCBI Official Synonym Full Names

chemokine (C-C motif) ligand 4

NCBI Official Symbol

Ccl4

NCBI Official Synonym Symbols

Act-2; Mip1b; Scya4; MIP-1B; AT744.1

UniProt Protein Name

C-C motif chemokine 4

UniProt Synonym Protein Names

Immune activation protein 2; ACT-2; ACT2; Macrophage inflammatory protein 1-beta; MIP-1-beta; Protein H400; SIS-gamma; Small-inducible cytokine A4

Protein Family

C-C motif chemokine

UniProt Gene Name

Ccl4

UniProt Synonym Gene Names

Mip1b; Scya4; ACT-2; ACT2; MIP-1-beta

UniProt Entry Name

CCL4_MOUSE

NCBI Protein Information

C-C motif chemokine 4; ACT2; MIP-1 beta; MIP-1-beta; SIS-gamma; immune activation protein 2; macrophage inflammatory protein 1-beta; small inducible cytokine A4; small-inducible cytokine A4

UniProt Comments for mMIP 1b

CCL4: Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5- mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: identical protein binding; protein binding; CCR1 chemokine receptor binding; chemokine activity; cytokine activity; CCR5 chemokine receptor binding

Biological Process: leukocyte chemotaxis; response to toxin; positive regulation of calcium-mediated signaling; immune response; positive regulation of calcium ion transport; positive regulation of tumor necrosis factor production; chemotaxis; inflammatory response; positive regulation of vascular permeability

Research Articles on mMIP 1b

1. CCL4 and CCL5 expression was higher in livers of infected WSX-1(-/-) mice than infected WT mice, and hepatic CD4 T cells from WSX-1(-/-) mice expressed higher levels of CCR5 than cells from WT mice

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with mMIP 1b active protein

Reference Product	PubMed Publications
Ccl3 active protein	>1620 publications with mMIP 1b and Ccl3
Ccl5 active protein	>997 publications with mMIP 1b and Ccl5
Ccr5 active protein	>593 publications with mMIP 1b and Ccr5
Ccr1 active protein	>198 publications with mMIP 1b and Ccr1
Ccr2 active protein	>108 publications with mMIP 1b and Ccr2
Ccl7 active protein	>77 publications with mMIP 1b and Ccl7
Cxcr3 active protein	>76 publications with mMIP 1b and Cxcr3
Ccr3 active protein	>71 publications with mMIP 1b and Ccr3
Ccr8 active protein	>24 publications with mMIP 1b and Ccr8
Ccl12 active protein	>13 publications with mMIP 1b and Ccl12

Pathways associated with mMIP 1b active protein

Products by Pathway	Pathway Diagram
Chemokine Receptors Bind Chemokines Pathway antibodies	Chemokine Receptors Bind Chemokines Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytosolic DNA-sensing Pathway antibodies	Cytosolic DNA-sensing Pathway Diagram
Cytosolic DNA-sensing Pathway antibodies	Cytosolic DNA-sensing Pathway Diagram
Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies	Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram

Diseases associated with mMIP 1b active protein

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>2684 publications with mMIP 1b and Liver Diseases
Poisoning Antibodies	>2010 publications with mMIP 1b and Poisoning
Drug-Induced Liver Injury Antibodies	>1618 publications with mMIP 1b and Drug-Induced Liver Injury
Disease Models, Animal Antibodies	>1291 publications with mMIP 1b and Disease Models, Animal
Necrosis Antibodies	>1214 publications with mMIP 1b and Necrosis
Drug-Related Side Effects and Adverse Reactions Antibodies	>1203 publications with mMIP 1b and Drug-Related Side Effects and Adverse Reactions
Inflammation Antibodies	>1103 publications with mMIP 1b and Inflammation
Fibrosis Antibodies	>955 publications with mMIP 1b and Fibrosis
Fatty Liver Antibodies	>291 publications with mMIP 1b and Fatty Liver
Lung Diseases Antibodies	>258 publications with mMIP 1b and Lung Diseases

Organs/Tissues associated with mMIP 1b active protein

Organ/Tissue Name	Pubmed Publications
Bone Marrow Antibodies	>300 publications with mMIP 1b and Bone Marrow
Spleen Antibodies	>220 publications with mMIP 1b and Spleen
Skin Antibodies	>141 publications with mMIP 1b and Skin
Eye Antibodies	>65 publications with mMIP 1b and Eye
Uncharacterized Tissue Antibodies	>1 publications with mMIP 1b and Uncharacterized Tissue