• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Meiotic Recombination Protein SPO11-1 (SPO11-1) Arabidopsis Thaliana Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1476948

MBS1476948 is a recombinant protein targeting SPO11-1. Also known as Recombinant Arabidopsis thaliana Meiotic recombination protein SPO11-1 (SPO11-1). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$1080 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Meiotic recombination protein SPO11-1 (SPO11-1), Recombinant Protein
Full Product Name
Recombinant Arabidopsis thaliana Meiotic recombination protein SPO11-1 (SPO11-1)
Product Gene Name
SPO11-1 recombinant protein
Product Synonym Gene Name
SPO11-1
Sequence
MEGKFAISES TNLLQRIKDF TQSVVVDLAE GRSPKISINQ FRNYCMNPEA DCLCSSDKPK GQEIFTLKKE PQTYRIDMLL RVLLIVQQLL QENRHASKRD IYYMHPSAFK AQSIVDRAIG DICILFQCSR YNLNVVSVGN GLVMGWLKFR EAGRKFDCLN SLNTAYPVPV LVEEVEDIVS LAEYILVVEK ETVFQRLAND MFCKTNRCIV ITGRGYPDVS TRRFLRLLME KLHLPVHCLV DCDPYGFEIL ATYRFGSMQM AYDIESLRAP DMKWLGAFPS DSEVYSVPKQ CLLPLTEEDK KRTEAMLLRC YLKREMPQWR LELETMLKRG VKFEIEALSV HSLSFLSEVY IPSKIRREVS SP
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
41,804 Da
Sequence Positions
1-362, Full length protein
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for Q9M4A2
Species
Arabidopsis thaliana (Mouse-ear cress)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of SPO11-1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SPO11-1. It may not necessarily be applicable to this product.

NCBI GI #
15231162
NCBI GeneID
820506
NCBI Accession #
NP_187923.1
UniProt Primary Accession #
Q9M4A2
UniProt Secondary Accession #
Q9C5S3; Q9LK56
UniProt Related Accession #
Q9M4A2
GenBank Nucleotide #
NM_112156.2
NCBI Official Full Name
Spo11/DNA topoisomerase VI, subunit A protein
NCBI Official Symbol
ATSPO11-1
NCBI Official Synonym Symbols
SPO11-1
UniProt Protein Name
Meiotic recombination protein SPO11-1
Protein Family
Meiotic recombination protein
UniProt Gene Name
SPO11-1
UniProt Synonym Gene Names
AtSPO11-1
NCBI Protein Information
Spo11/DNA topoisomerase VI, subunit A protein

NCBI Summary for SPO11-1

Encodes AtSPO11-1, one of the three Arabidopsis homologues of the archaeal DNA topoisomerase VIA subunit (topo VIA). Required for meiotic recombination. AtSPO11-1 and AtSPO11-2 have overlapping functions (i.e. both required for meiotic recombination) whereas AtSPO11-3 functions in DNA replication. AtSPO11-1 accumulates in foci in early G2. At 1 h post-S phase, no foci are observed, but by 3 h a majority (80%) of meiocytes at this time point contain >50 foci. However, by 5 h, AtSPO11-1 foci are no longer detectable. This suggests that the protein undergoes a rapid cycle of accumulation and disappearance in meiocytes over a period of between 1 and 5 h post-S phase.
Show more

UniProt Comments for SPO11-1

Component of a topoisomerase 6 complex specifically required for meiotic recombination (PubMed:11157765, PubMed:17018031, PubMed:17965269, PubMed:26917763). Together with MTOPVIB, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (PubMed:26917763). The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles (PubMed:11157765, PubMed:17018031, PubMed:17965269, PubMed:26917763).
Show more

Research Articles on SPO11-1

1. Results identified a group of spo11-1 mutants with defects in meiosis that specifically correlate with the defects in DNA binding at an identified DNA binding surface on SPO11-1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with SPO11-1 recombinant protein

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Meiosis Pathway antibodies  Meiosis Pathway Diagram
 Meiotic Recombination Pathway antibodies  Meiotic Recombination Pathway Diagram
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000