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BHLHA15 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS153611

BHLHA15 (basic helix-loop-helix protein 15), also known as MIST1 (muscle intestine and stomach expression 1) belongs to the bHLH family of transcription factors and plays a role in regulating the transcriptional activity of MYOD1 in muscle cell development as… More details

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Product Overview

Online Datasheet in PDF
Product Name
BHLHA15, Polyclonal Antibody
Full Product Name
BHLHA15 Antibody
Product Gene Name
anti-BHLHA15 antibody
Product Synonym Names
BHLHA15; MIST1; BHLHB8; MIST1; Class A basic helix-loop-helix protein 15; Class B basic helix-loop-helix protein 8; bHLHa15; basic helix-loop-helix family, member a15
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
BHLHA15 antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
BHLHA15 antibody is human, mouse and rat reactive.
Preparation and Storage
BHLHA15 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Antibody/Peptide Pairs
BHLHA15 peptide (MBS153989) is used for blocking the activity of BHLHA15 antibody (MBS153611)
Molecular Weight
Predicted: 21 kDa

Observed: 24 kDa
Immunogen Sequence Positions
60-110
OMIM
608606
3D Structure
ModBase 3D Structure for Q7RTS1
Conjugate
Unconjugated
Immunogen
BHLHA15 antibody was raised against a 18 amino acid peptide near the center of human BHLHA15.
Buffer
BHLHA15 antibody is supplied in PBS containing 0.02% sodium azide.
Positive Control
Rat Small Intestine Tissue Lysate: MBS151680
Human small Intestine Tissue Slide: MBS154284
Homology
Predicted species reactivity based on immunogen sequence: Bovine: (71%)
Applications Tested/Suitable for anti-BHLHA15 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-BHLHA15 antibody
BHLHA15 antibody can be used for detection of BHLHA15 by Western blot at 1 - 2 ug/ml. Antibody can also be used for Immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL.

Western Blot (WB) of anti-BHLHA15 antibody

Western blot analysis of BHLHA15 in rat small Intestine tissue lysate with BHLHA15 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide
Rabbit Anti-BHLHA15 Polyclonal Antibody – Cat# MBS153611

Immunohistochemistry (IHC) of anti-BHLHA15 antibody

Immunohistochemistry of BHLHA15 in human small intestine tissue with BHLHA15 antibody at 5 μg/mL.
Rabbit Anti-BHLHA15 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS153611

Immunofluorescence (IF) of anti-BHLHA15 antibody

Immunofluorescence of BHLHA15 in human small intestine tissue with BHLHA15 antibody at 20 μg/mL.
Rabbit Anti-BHLHA15 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS153611

Other Notes

Small volumes of anti-BHLHA15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BHLHA15 purchase

MBS153611 is an antibody targeting BHLHA15. Also known as BHLHA15 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BHLHA15. It may not necessarily be applicable to this product.

NCBI GI #
29126247
NCBI GeneID
168620
NCBI Accession #
NP_803238
UniProt Primary Accession #
Q7RTS1
UniProt Secondary Accession #
Q14DE4; A4D271
UniProt Related Accession #
Q7RTS1
GenBank Nucleotide #
NM_177455.3
NCBI Official Full Name
class A basic helix-loop-helix protein 15
NCBI Official Synonym Full Names
basic helix-loop-helix family, member a15
NCBI Official Symbol
BHLHA15
NCBI Official Synonym Symbols
MIST1; BHLHB8
UniProt Protein Name
Class A basic helix-loop-helix protein 15
UniProt Synonym Protein Names
Class B basic helix-loop-helix protein 8; bHLHb8; Muscle, intestine and stomach expression 1; MIST-1
Protein Family
Class A basic helix-loop-helix protein
UniProt Gene Name
BHLHA15
UniProt Synonym Gene Names
BHLHB8; MIST1; bHLHa15; bHLHb8; MIST-1
UniProt Entry Name
BHA15_HUMAN
NCBI Protein Information
class A basic helix-loop-helix protein 15; MIST-1; class II bHLH protein MIST1; class B basic helix-loop-helix protein 8; muscle, intestine and stomach expression 1; basic helix-loop-helix domain containing, class B, 8

UniProt Comments for BHLHA15

MIST1: Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E- box occupancy by homodimers. May also negatively regulate bHLH- mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E- box consensus sequence 5'-CANNTG-3'.

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: nucleus

Molecular Function: protein homodimerization activity

Biological Process: G-protein coupled receptor protein signaling pathway; cellular response to glucose starvation; transcription, DNA-dependent; cell-cell signaling; unfolded protein response; cell maturation; intracellular distribution of mitochondria; calcium-mediated signaling; positive regulation of transcription from RNA polymerase II promoter; glucose homeostasis; Golgi organization and biogenesis; mitochondrial calcium ion transport
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Research Articles on BHLHA15

1. noted a higher mean value of microvascular density in CK5/6 and Ep-CAM-immunopositive tumors, carcinomas with aberrant CK8/18 expression, and carcinomas with no or strong expression of MMP-2 in stromal fibroblast-like cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-BHLHA15 antibody

 Products by Pathway  Pathway Diagram
 Maturity Onset Diabetes Of The Young Pathway antibodies  Maturity Onset Diabetes Of The Young Pathway Diagram
 Maturity Onset Diabetes Of The Young Pathway antibodies  Maturity Onset Diabetes Of The Young Pathway Diagram

Diseases associated with anti-BHLHA15 antibody

 Disease Name  Pubmed Publications
 Pancreatitis Antibodies  >1 publications with BHLHA15 and Pancreatitis
 Disease Models, Animal Antibodies  >1 publications with BHLHA15 and Disease Models, Animal
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