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PIN1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1756542

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, also called DOD, is an enzyme that in humans is encoded by the PIN1 gene. It is mapped to 19p13.2. The enzyme binds to a subset of proteins and thus plays a role as a post phospho PIN1rol in regulating… More details

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Product Overview

Online Datasheet in PDF
Product Name
Pin1, Monoclonal Antibody
Full Product Name
Anti-Pin1 Antibody Picoband (monoclonal, 5E5)
Product Gene Name
anti-PIN1 antibody
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
5E5
Host
Mouse
Species Reactivity
Human, Monkey, Rat
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (lot specific)
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Chromosome Location
Chromosome: 19; NC_000019.9 (9945883..9960365). Location: 19p13
OMIM
601052
3D Structure
ModBase 3D Structure for Q13526
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Immunogen
E Coli-derived human Pin1 recombinant protein (Position: M1-E163). Human Pin1 shares 95% amino acid (aa) sequence identity with mouse Pin1.

Product Categories/Family for anti-PIN1 antibody

Primary Antibodies, Mouse Monoclonal Antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-PIN1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-PIN1 antibody
WB: 0.25-0.5 ug/ml, Human, Monkey, Rat
ICC/IF: 5 ug/ml, Human
FC/FACS: 1-3 ug/1x10^6 cells, Human
Tested Species: In-house tested species with positive results.
Enhanced Chemiluminescent Kit with anti-Mouse IgG (#MBS176445) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (#MBS176472) for ICC.

Western Blot (WB) of anti-PIN1 antibody

Figure 1. Western blot analysis of Pin1 using anti-Pin1 antibody (MBS1756542).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.

Lane 1: human Hela whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates.

After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Pin1 antigen affinity purified monoclonal antibody (#MBS1756542) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Pin1 at approximately 18 kDa. The expected band size for Pin1 is at 18 kDa.
Mouse Anti-PIN1 Monoclonal Antibody – Cat# MBS1756542

Immunofluorescence (IF) of anti-PIN1 antibody

Figure 2. IF analysis of Pin1 using anti-Pin1 antibody (MBS1756542).

Pin1 was detected in an immunocytochemical section of HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-Pin1 Antibody (MBS1756542) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG (MBS177407) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Mouse Anti-PIN1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS1756542

Flow Cytometry (FC/FACS) of anti-PIN1 antibody

Figure 3. Flow Cytometry analysis of U87 cells using anti-Pin1 antibody (MBS1756542).

Overlay histogram showing U87 cells stained with MBS1756542 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Pin1 Antibody (MBS1756542, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG (MBS177407, 5-10 ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Mouse Anti-PIN1 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1756542

Other Notes

Small volumes of anti-PIN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PIN1 purchase

MBS1756542 is an antibody targeting PIN1. Also known as Anti-Pin1 Antibody Picoband (monoclonal, 5E5). Reactive in Human, Monkey, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PIN1. It may not necessarily be applicable to this product.

NCBI GI #
5453898
NCBI GeneID
5300
NCBI Accession #
NP_006212.1
UniProt Primary Accession #
Q13526
UniProt Secondary Accession #
Q53X75; A8K4V9
UniProt Related Accession #
Q13526
GenBank Nucleotide #
NM_006221.3
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
NCBI Official Synonym Full Names
peptidylprolyl cis/trans isomerase, NIMA-interacting 1
NCBI Official Symbol
PIN1
NCBI Official Synonym Symbols
DOD; UBL5
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
UniProt Synonym Protein Names
Peptidyl-prolyl cis-trans isomerase Pin1; PPIase Pin1; Rotamase Pin1
Protein Family
Auxin efflux carrier component
UniProt Gene Name
PIN1
UniProt Synonym Gene Names
PPIase Pin1
UniProt Entry Name
PIN1_HUMAN
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; PPIase Pin1; rotamase Pin1; protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1

NCBI Summary for PIN1

Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, induction and maintenance of pluripotency, germ cell development, neuronal differentiation, and survival. This enzyme also plays a key role in the pathogenesis of Alzheimer's disease and many cancers. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]
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UniProt Comments for PIN1

PIN1: a member of the parvulin family of peptidyl-prolyl isomerases (PPIase), has been implicated in the G2-M transition of the cell cycle. Has two distinct functional domains: an N-terminal WW domain and a C-terminal PPlase domain. Pin1 interacts with a series of mitotic phosphoproteins, including Plk1, cdc25C and cdc27, catalyzing pSer-Pro or pThr/Pro cis/trans isomerizations. Displays a preference for an acidic residue n-terminal to the isomerized proline bond.

Protein type: Nuclear receptor co-regulator; EC 5.2.1.8; Isomerase

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: nucleoplasm; cytoplasm; nuclear speck; midbody; nucleus

Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; mitogen-activated protein kinase kinase binding; phosphothreonine binding; GTPase activating protein binding; phosphoserine binding

Biological Process: protein peptidyl-prolyl isomerization; positive regulation of ubiquitin-protein ligase activity; cytokine and chemokine mediated signaling pathway; regulation of mitosis; innate immune response; positive regulation of protein amino acid phosphorylation; cell cycle; negative regulation of transforming growth factor beta receptor signaling pathway; regulation of cytokinesis; negative regulation of interferon type I production
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Research Articles on PIN1

1. the phosphorylated peptides of the Bcl-2 loop can bind Pin1, further substantiating the phosphorylation-mediated conformation change of Bcl-2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PIN1 antibody

 Reference Product  PubMed Publications
 JUN antibody  >101 publications with PIN1 and JUN
 CCND1 antibody  >38 publications with PIN1 and CCND1
 CTNNB1 antibody  >26 publications with PIN1 and CTNNB1
 APP antibody  >17 publications with PIN1 and APP
 CDC25C antibody  >9 publications with PIN1 and CDC25C
 PLK1 antibody  >7 publications with PIN1 and PLK1
 MAPT antibody  >7 publications with PIN1 and MAPT
 RELA antibody  >6 publications with PIN1 and RELA
 CDK1 antibody  >5 publications with PIN1 and CDK1
 CCNB1 antibody  >4 publications with PIN1 and CCNB1

Pathways associated with anti-PIN1 antibody

 Products by Pathway  Pathway Diagram
 Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies  Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
 C-MYC Pathway antibodies  C-MYC Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 ISG15 Antiviral Mechanism Pathway antibodies  ISG15 Antiviral Mechanism Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Interferon Signaling Pathway antibodies  Interferon Signaling Pathway Diagram
 Negative Regulators Of RIG-I/MDA5 Signaling Pathway antibodies  Negative Regulators Of RIG-I/MDA5 Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram

Diseases associated with anti-PIN1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >151 publications with PIN1 and Neoplasms
 Brain Diseases Antibodies  >77 publications with PIN1 and Brain Diseases
 Breast Neoplasms Antibodies  >40 publications with PIN1 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >37 publications with PIN1 and Cell Transformation, Neoplastic
 Inflammation Antibodies  >19 publications with PIN1 and Inflammation
 Neoplasm Metastasis Antibodies  >15 publications with PIN1 and Neoplasm Metastasis
 Liver Diseases Antibodies  >14 publications with PIN1 and Liver Diseases
 Prostatic Diseases Antibodies  >13 publications with PIN1 and Prostatic Diseases
 Carcinoma, Squamous Cell Antibodies  >12 publications with PIN1 and Carcinoma, Squamous Cell
 Lung Neoplasms Antibodies  >12 publications with PIN1 and Lung Neoplasms

Organs/Tissues associated with anti-PIN1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >71 publications with PIN1 and Brain
 Blood Antibodies  >35 publications with PIN1 and Blood
 Lung Antibodies  >25 publications with PIN1 and Lung
 Liver Antibodies  >22 publications with PIN1 and Liver
 Prostate Antibodies  >19 publications with PIN1 and Prostate
 Nerve Antibodies  >18 publications with PIN1 and Nerve
 Kidney Antibodies  >15 publications with PIN1 and Kidney
 Embryonic Tissue Antibodies  >13 publications with PIN1 and Embryonic Tissue
 Lymph Node Antibodies  >13 publications with PIN1 and Lymph Node
 Lymph Antibodies  >12 publications with PIN1 and Lymph
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