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RNH1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200161

Ribonuclease inhibitor 1 (RNH1) the placental ribonuclease inhibitor, is an acid protein with a molecular weight of 50 kDa. RNH1 is a member of a family of proteinaceous cytoplasmic RNase inhibitors that are expressed in many tissues and bind to both… More details

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Product Overview

Online Datasheet in PDF
Product Name
RNH1, Monoclonal Antibody
Full Product Name
RNH1 antibody
Product Gene Name
Product Synonym Names
ribonuclease/angiogenin inhibitor 1; ribonuclease/angiogenin inhibitor; RAI
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT1H23
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
49,973 Da
OMIM
M22414 mRNA
3D Structure
ModBase 3D Structure for P13489
Antigen Species
Human
Immunogen
Recombinant Ribonuclease inhibitor 1 (RNH1) (7-461aa) purified from E. coli
Clone Note
Anti-human RNH1 mAb, clone AT1H23, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human RNH1 protein.

Product Categories/Family for anti-RNH1 antibody

Applications Tested/Suitable for anti-RNH1 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-RNH1 antibody
WB: 1:1000 ~ 2000.
Recommended starting dilution is 1:1000.

Western Blot (WB) of anti-RNH1 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RNH1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Jurkat cell lysate Lane 2. : HepG2 cell lysate
Mouse Anti-RNH1 Monoclonal Antibody – Cat# MBS200161

Immunofluorescence (IF) of anti-RNH1 antibody

ICC/IF analysis of RNH1 in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RNH1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-RNH1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200161

Product References and Citations for anti-RNH1 antibody

Park J.H., et al., (2008) Cytotechnology 57 (1):93-99 ; Johnson R.J., et al., (2007) J Mol Biol 368 (2):434-449; Fu P, et al., (2005) Cancer Gene Ther 12 (3):268-275

Other Notes

Small volumes of anti-RNH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RNH1 purchase

MBS200161 is an antibody targeting RNH1. Also known as RNH1 antibody. Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for RNH1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ribonuclease inhibitor
NCBI Official Synonym Full Names
ribonuclease/angiogenin inhibitor 1
NCBI Official Symbol
RNH1
NCBI Official Synonym Symbols
RAI; RNH
UniProt Protein Name
Ribonuclease inhibitor
UniProt Synonym Protein Names
Placental ribonuclease inhibitor; Placental RNase inhibitor; Ribonuclease/angiogenin inhibitor 1; RAI
Protein Family
UniProt Gene Name
RNH1
UniProt Synonym Gene Names
PRI; RNH; Placental RNase inhibitor; RAI
NCBI Protein Information
ribonuclease inhibitor

NCBI Summary for RNH1

Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM, Jul 2010]
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UniProt Comments for RNH1

Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play a role in redox homeostasis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RNH1 antibody

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