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Apolipoprotein A4 (APOA4) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2002143
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MBS2002143 is an antibody targeting APOA4. Also known as Polyclonal Antibody to Apolipoprotein A4 (APOA4). Reactive in Mouse. Host: Rabbit. More details

$210 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Apolipoprotein A4 (APOA4), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Apolipoprotein A4 (APOA4)
Product Gene Name
anti-APOA4 antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Concentration
0.93mg/ml (lot specific)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: APOA4 (MBS2002143)
Biotin Conjugated Antibody: APOA4 (MBS2004782)
Matching Pairs
Unconjugated Antibody: APOA4 (MBS2002143)
Immunogen: APOA4 (MBS2009973)
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3D Structure
ModBase 3D Structure for P06728
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Organism Species
Mus musculus (Mouse)
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Mouse
Immunogen
Recombinant APOA4 (Gln299~Ala369) expressed in E.coli (MBS2009973)
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-APOA4 antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemsitry (ICC); Immunoprecipitation (IP).
Application Notes for anti-APOA4 antibody
Western blotting: 0.5-2ug/mL
I mmunohistochemistry: 5-20ug/mL
I mmunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-APOA4 antibody

Western Blot; Sample: Recombinant APOA4, Mouse.
Rabbit Anti-APOA4 Polyclonal Antibody – Cat# MBS2002143

Western Blot (WB) of anti-APOA4 antibody

Western Blot; Sample: Mouse Lung lysate;
Primary Ab: 1ug/ml Rabbit Anti-Mouse APOA4 Antibody
Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Rabbit Anti-APOA4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2002143

Immunohistochemistry (IHC) of anti-APOA4 antibody

DAB staining on IHC-P; Samples: Mouse Intestine Tissue.
Rabbit Anti-APOA4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002143

Other Notes

Small volumes of anti-APOA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for APOA4. It may not necessarily be applicable to this product.

NCBI GI #
110347473
NCBI GeneID
11808
NCBI Accession #
NP_031494.2
UniProt Primary Accession #
P06728
UniProt Secondary Accession #
Q91XF8
UniProt Related Accession #
P06728
GenBank Nucleotide #
NM_007468.2
NCBI Official Full Name
apolipoprotein A-IV
NCBI Official Synonym Full Names
apolipoprotein A-IV
NCBI Official Symbol
Apoa4
NCBI Official Synonym Symbols
Apoa-4
UniProt Protein Name
Apolipoprotein A-IV
UniProt Synonym Protein Names
Apolipoprotein A4
Protein Family
Apolipoprotein
UniProt Gene Name
Apoa4
UniProt Synonym Gene Names
Apo-AIV; ApoA-IV
NCBI Protein Information
apolipoprotein A-IV

UniProt Comments for APOA4

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Cell adhesion; Endoplasmic reticulum; Lipid-binding; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9 A5.2|9 25.36 cM

Cellular Component: cell surface; chylomicron; extracellular matrix; extracellular region; extracellular space

Molecular Function: antioxidant activity; cholesterol binding; cholesterol transporter activity; copper ion binding; lipid binding; phosphatidylcholine binding; protein homodimerization activity

Biological Process: cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; hydrogen peroxide catabolic process; innate immune response in mucosa; leukocyte adhesion; lipid homeostasis; lipid transport; lipoprotein metabolic process; multicellular organismal lipid catabolic process; neuron projection regeneration; phosphatidylcholine metabolic process; phospholipid efflux; positive regulation of fatty acid biosynthetic process; positive regulation of lipoprotein lipase activity; protein-lipid complex assembly; regulation of cholesterol transport; regulation of intestinal cholesterol absorption; removal of superoxide radicals; response to lipid hydroperoxide; reverse cholesterol transport; transport; triglyceride catabolic process
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Research Articles on APOA4

1. This study uncovers an anti-sense lncRNA (APOA4-AS), which is co-expressed with APOA4, and concordantly and specifically regulates APOA4 expression both in vitro and in vivo with the involvement of HuR.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-APOA4 antibody

 Products by Pathway  Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Lipoprotein Metabolism Pathway antibodies  Lipoprotein Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Fat-soluble Vitamins Pathway antibodies  Metabolism Of Fat-soluble Vitamins Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Metabolism Of Vitamins And Cofactors Pathway antibodies  Metabolism Of Vitamins And Cofactors Pathway Diagram
 Retinoid Metabolism And Transport Pathway antibodies  Retinoid Metabolism And Transport Pathway Diagram

Diseases associated with anti-APOA4 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >28 publications with APOA4 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >16 publications with APOA4 and Atherosclerosis
 Heart Diseases Antibodies  >15 publications with APOA4 and Heart Diseases
 Dyslipidemias Antibodies  >14 publications with APOA4 and Dyslipidemias
 Hyperlipidemias Antibodies  >12 publications with APOA4 and Hyperlipidemias
 Fatty Liver Antibodies  >8 publications with APOA4 and Fatty Liver
 Inflammation Antibodies  >7 publications with APOA4 and Inflammation
 Liver Diseases Antibodies  >6 publications with APOA4 and Liver Diseases
 Immune System Diseases Antibodies  >4 publications with APOA4 and Immune System Diseases
 Brain Diseases Antibodies  >3 publications with APOA4 and Brain Diseases

Organs/Tissues associated with anti-APOA4 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >27 publications with APOA4 and Liver
 Heart Antibodies  >16 publications with APOA4 and Heart
 Intestine Antibodies  >10 publications with APOA4 and Intestine
 Muscle Antibodies  >4 publications with APOA4 and Muscle
 Kidney Antibodies  >2 publications with APOA4 and Kidney
 Pancreas Antibodies  >2 publications with APOA4 and Pancreas
 Stomach Antibodies  >1 publications with APOA4 and Stomach
 Ovary Antibodies  >1 publications with APOA4 and Ovary
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