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CD11b Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS216534

Rat anti Mouse CD11b antibody, clone 5C6 recognizes CD11b, also known as the integrin alpha M chain. CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated… More details

$255 / 0.025 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD11b, Monoclonal Antibody
Full Product Name
RAT ANTI MOUSE CD11b
Product Gene Name
anti-CD11b antibody
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
5C6
Host
Rat
Form/Format
Purified
Purified IgG - liquid; Phosphate buffered saline
Concentration
IgG concentration 1 mg/ml (lot specific)
Preparation and Storage
It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
3D Structure
ModBase 3D Structure for P05555
Immunogen
Thioglycollate-elicited peritoneal macrophages (TPM)
Preservative Stabilisers
0.09% Sodium Azide
Species Cross Reactivity
Reacts with: Human
N.B. Antibody reactivity and working conditions may vary between species. Cross reactivity is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Target Species
Mouse
Fusion Partners
Spleen cells from AO rats were fused with cells of the Y3 rat myeloma cell line
Histology Positive Control Tissue
Spleen
Applications Tested/Suitable for anti-CD11b antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-CD11b antibody
Flow Cytometry: suggested dilution of 1/100; Use 10ul of the suggested working dilution to label 10

Other Notes

Small volumes of anti-CD11b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD11b purchase

MBS216534 is an antibody targeting CD11b. Also known as RAT ANTI MOUSE CD11b. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD11b. It may not necessarily be applicable to this product.

NCBI GI #
124956
NCBI GeneID
16409
NCBI Accession #
P05555.2
UniProt Primary Accession #
P05555
UniProt Secondary Accession #
Q8CA73
UniProt Related Accession #
P05555
NCBI Official Full Name
Integrin alpha-M
NCBI Official Synonym Full Names
integrin alpha M
NCBI Official Symbol
Itgam
NCBI Official Synonym Symbols
CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
UniProt Protein Name
Integrin alpha-M
UniProt Synonym Protein Names
CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; CD_antigen: CD11b
Protein Family
Integrin
UniProt Gene Name
Itgam
UniProt Entry Name
ITAM_MOUSE
NCBI Protein Information
integrin alpha-M; CD11 antigen-like family member B; CD11B (p170); CR-3 alpha chain; Mac-1 alpha; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component receptor 3 alpha-a; complement receptor type 3; leukocyte adhesion receptor MO1; macrophage antigen alpha

UniProt Comments for CD11b

ITGAM: a single-pass type I membrane protein of the integrin alpha chain family. Implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. Also known as CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. A receptor for fibrinogen, factor X and ICAM1. Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. Predominantly expressed in monocytes and granulocytes.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Cellular Component: cell surface; membrane; integral to membrane; integrin complex; nucleus; external side of plasma membrane

Molecular Function: heparin binding; heparan sulfate proteoglycan binding; opsonin binding; protein heterodimerization activity; metal ion binding; glycoprotein binding

Biological Process: integrin-mediated signaling pathway; neutrophil chemotaxis; leukocyte adhesion; cell-cell adhesion; microglia development; cellular extravasation; activated T cell proliferation; cell adhesion; leukocyte migration during inflammatory response
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Research Articles on CD11b

1. These findings indicate that inhibition of indoleamine 2,3-dioxygenase-1 reduces mortality from peritonitis and sepsis after CLP via recruitment of neutrophils and mononuclear cells by chemokine production in peritoneal CD11b(+) cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD11b antibody

 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram

Organs/Tissues associated with anti-CD11b antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4528 publications with CD11b and Blood
 Bone Marrow Antibodies  >798 publications with CD11b and Bone Marrow
 Brain Antibodies  >631 publications with CD11b and Brain
 Lung Antibodies  >545 publications with CD11b and Lung
 Spleen Antibodies  >519 publications with CD11b and Spleen
 Skin Antibodies  >248 publications with CD11b and Skin
 Heart Antibodies  >211 publications with CD11b and Heart
 Spinal Cord Antibodies  >164 publications with CD11b and Spinal Cord
 Intestine Antibodies  >161 publications with CD11b and Intestine
 Eye Antibodies  >152 publications with CD11b and Eye
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