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Glutamate Decarboxylase 1/2 (GAD2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS221561

This product recognises both molecular forms of glutamate decarboxylase, GAD1 (GAD67) and GAD2 (GAD65), the enzyme which converts glutamic acid to gamma -aminobutyric acid (GABA), the major inhibitory transmitter in the higher brain region. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GLUTAMATE DECARBOXYLASE 1/2 (Gad2), Polyclonal Antibody
Full Product Name
RABBIT ANTI GLUTAMATE DECARBOXYLASE 1/2
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Cat, Human, Mouse
Form/Format
Purified
Purified IgG - liquid
Specificity
This product recognises both molecular forms of glutamate decarboxylase, GAD1 (GAD67) and GAD2 (GAD65), the enzyme which converts glutamic acid to gamma -aminobutyric acid (GABA), the major inhibitory transmitter in the higher brain region.
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
65,402 Da
3D Structure
ModBase 3D Structure for Q05683
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide sequence D-F-L-I-E-E-I-E-R-L-G-Q-D-L from the C terminus of rat GAD2
Histology Positive Control Tissue||Brain
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antiserum to glutamic acid decarboxylase was raised by repeated immunisation of with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on protein A.
Target Species
Rat
Applications Tested/Suitable for anti-Gad2 antibody
Immunohistology Frozen, ELISA (EIA), Immunofluorescence (IF), Immunohistology Paraffin, Western Blot (WB)
Application Notes for anti-Gad2 antibody
Western Blot: A doublet at approximately 65/67 kDa can be expected
Immunohistology - Frozen: Minimum Dilution: 1/200; Maximum Dilution: 1/5000
Western Blotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/10000

Other Notes

Small volumes of anti-Gad2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Gad2 purchase

MBS221561 is an antibody targeting Gad2. Also known as RABBIT ANTI GLUTAMATE DECARBOXYLASE 1/2. Reactive in Cat, Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Gad2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
glutamate decarboxylase 2
NCBI Official Synonym Full Names
glutamate decarboxylase 2
NCBI Official Symbol
Gad2
NCBI Official Synonym Symbols
gad65
UniProt Protein Name
Glutamate decarboxylase 2
UniProt Synonym Protein Names
65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform
UniProt Gene Name
Gad2
UniProt Synonym Gene Names
Gad65; GAD-65
UniProt Entry Name
DCE2_RAT
NCBI Protein Information
glutamate decarboxylase 2; 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 2 (islet); glutamate decarboxylase 65 kDa isoform; glutamic acid decarboxylase 2; glutamic acid decarboxylase 65

NCBI Summary for Gad2

catalyzes synthesis of gamma-Aminobutyric acid (GABA), the most widely distributed inhibitory neurotransmitter [RGD, Feb 2006]

UniProt Comments for Gad2

GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.

Protein type: Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - alanine, aspartate and glutamate; Lyase; EC 4.1.1.15; Other Amino Acids Metabolism - taurine and hypotaurine

Cellular Component: Golgi membrane; presynaptic membrane; synaptic vesicle membrane; perinuclear region of cytoplasm; axon; cytoplasm; synapse; cytoplasmic vesicle; cell junction; cytosol

Molecular Function: glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding

Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter biosynthetic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Gad2 antibody

 Reference Product  PubMed Publications
 Abat antibody  >1 publications with Gad2 and Abat

Organs/Tissues associated with anti-Gad2 antibody

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