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APOM Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS244104

MBS244104 is an antibody targeting APOM. Also known as Goat Polyclonal to Mouse APOM / Apolipoprotein M. Reactive in Human, MousePredicted Reactivity: Rat (at least 90% immunogen sequence identity). Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
APOM / Apolipoprotein M, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Mouse APOM / Apolipoprotein M
Product Gene Name
Product Synonym Names
Anti-APOM / Apolipoprotein M Antibody (Internal) IHC-plus; APOM; Alternative name: G3a; NG20; Apo-M; Apolipoprotein M; HSPC336; G3a; NG20; NG20-like protein; Protein G3a; Mouse APOM; Apolipoprotein M
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human, Mouse
Predicted Reactivity: Rat (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Mouse APOM / Apolipoprotein M.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
13,051 Da
OMIM
606907
3D Structure
ModBase 3D Structure for O95445
Target Species
Mouse
Immunogen Description
Synthetic peptide C-KGNMELRTEGRPD from an internal region of mouse APOM / Apolipoprotein M (NP_061286.1). Percent identity with other species by BLAST analysis: Mouse (100%), Rat (92%).
Immunogen Type
Synthetic peptide
Immunogen
APOM / Apolipoprotein M antibody was raised against synthetic peptide C-KGNMELRTEGRPD from an internal region of mouse APOM / Apolipoprotein M (NP_061286.1). Percent identity with other species by BLAST analysis: Mouse (100%), Rat (92%).
Antigen Modification
Internal
Applications Tested/Suitable for anti-APOM antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-APOM antibody
ELISA (1:16000), IHC-P (3.75 ug/ml), WB (0.3 - 1 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx. 26kDa band observed in Human Placenta lysates (calculated MW of 21.3kDa according to NP_061286.1). The observed molecular weight corresponds to earlier findings in litera...

Immunohistochemistry (IHC) of anti-APOM antibody

Anti-APOM / Apolipoprotein M antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Goat Anti-APOM Polyclonal Antibody – Cat# MBS244104

Western Blot (WB) of anti-APOM antibody

APOM antibody (0.5 ug/ml) staining of human placenta lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-APOM Polyclonal Antibody, Western Blot (WB) – Cat# MBS244104

Other Notes

Small volumes of anti-APOM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for APOM. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
apolipoprotein M isoform 1
NCBI Official Synonym Full Names
apolipoprotein M
NCBI Official Symbol
APOM
NCBI Official Synonym Symbols
G3a; NG20; apo-M; HSPC336
UniProt Protein Name
Apolipoprotein M
UniProt Synonym Protein Names
Protein G3a
Protein Family
UniProt Gene Name
APOM
UniProt Synonym Gene Names
G3A; NG20; Apo-M; ApoM
UniProt Entry Name
APOM_HUMAN
NCBI Protein Information
apolipoprotein M; protein G3a; NG20-like protein; alternative name: G3a, NG20

NCBI Summary for APOM

The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Alternate splicing results in both coding and non-coding variants of this gene. [provided by RefSeq, Jan 2012]
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UniProt Comments for APOM

APOM: Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid. Plasma protein. Expressed in liver and kidney. Belongs to the calycin superfamily. Lipocalin family. Highly divergent.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: integral to plasma membrane

Molecular Function: antioxidant activity; lipid transporter activity; phospholipid binding

Biological Process: cholesterol homeostasis; reverse cholesterol transport; response to glucose stimulus; cholesterol efflux; lipoprotein metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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