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IL25 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245013

MBS245013 is an antibody targeting IL25. Also known as Mouse Monoclonal [clone 68C1039.2] (IgG1) to Human IL25 / IL17E. Reactive in Human, Mouse. Host: Mouse. More details

$650 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IL25 / IL17E, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 68C1039.2] (IgG1) to Human IL25 / IL17E
Product Gene Name
anti-IL25 antibody
Product Synonym Gene Name
IL17E
Product Synonym Names
Anti-IL25 / IL17E Antibody (aa115-132, clone 68C1039.2) IHC-plus; IL25; Interleukin 17E; Interleukin 25; Interleukin-25; IL17E; IL-17E; IL-25; Interleukin-17E; Human IL25; IL17E
Clonality
Monoclonal
Isotype
IgG1
Clone Number
68C1039.2
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
PBS with 0.05% sodium azide.
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Specificity
A synthetic peptide of amino acids 115-132, obtained from the human IL-17E protein sequence, was used as immunogen
Preparation and Storage
Short term: Store at 4°C. Long term: aliquot and store at -20C. Avoid freeze/thaw cycles.
OMIM
605658
3D Structure
ModBase 3D Structure for Q9H293
Immunogen Species
IL25 / IL17E antibody was raised against Human
Usage
Immunohistochemistry: MBS245013 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody , followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS245013 was determined to be 10 ug/ml.
Immunogen Type
Synthetic peptide
Immunogen
IL25 / IL17E antibody was raised against synthetic peptide from human IL25 / IL-25.
Epitope
aa115-132
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-IL25 antibody

Family: Interleukin
Applications Tested/Suitable for anti-IL25 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-IL25 antibody
IHC-P (10 ug/ml), WB (2-4 ug/ml)
Optimal dilution to be determine by the researcher.

Immunohistochemistry (IHC) of anti-IL25 antibody

Anti-IL-25 antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-IL25 Monoclonal Antibody – Cat# MBS245013

Other Notes

Small volumes of anti-IL25 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IL25. It may not necessarily be applicable to this product.

NCBI GI #
12232485
NCBI GeneID
64806
NCBI Accession #
NP_073626.1
UniProt Primary Accession #
Q9H293
UniProt Secondary Accession #
Q2M3F0; Q8IZV3; Q8WXB0
UniProt Related Accession #
Q9H293
GenBank Nucleotide #
NM_022789.3
NCBI Official Full Name
interleukin-25 isoform 1
NCBI Official Synonym Full Names
interleukin 25
NCBI Official Symbol
IL25
NCBI Official Synonym Symbols
IL17E
UniProt Protein Name
Interleukin-25
UniProt Synonym Protein Names
Interleukin-17E; IL-17E
Protein Family
Interleukin
UniProt Gene Name
IL25
UniProt Synonym Gene Names
IL17E; IL-25; IL-17E
UniProt Entry Name
IL25_HUMAN
NCBI Protein Information
interleukin-25; interleukin-17E

NCBI Summary for IL25

The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
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UniProt Comments for IL25

IL25: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. Belongs to the IL-17 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular space

Molecular Function: cytokine activity; interleukin-17E receptor binding

Biological Process: response to fungus; inflammatory response to antigenic stimulus; response to nematode; eosinophil differentiation; positive regulation of transcription from RNA polymerase II promoter
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Research Articles on IL25

1. Because IL-25 has a major role in triggering type 2 responses, bronchial epithelial IL-25 expression is likely a key determinant of type 2 response activation in asthma

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IL25 antibody

 Reference Product  PubMed Publications
 IL33 antibody  >14 publications with IL25 and IL33
 IL17RB antibody  >3 publications with IL25 and IL17RB
 ITGAX antibody  >2 publications with IL25 and ITGAX
 IL23A antibody  >1 publications with IL25 and IL23A
 CCL11 antibody  >1 publications with IL25 and CCL11

Pathways associated with anti-IL25 antibody

 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 IL17 Signaling Pathway antibodies  IL17 Signaling Pathway Diagram

Diseases associated with anti-IL25 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >33 publications with IL25 and Inflammation
 Hypersensitivity Antibodies  >28 publications with IL25 and Hypersensitivity
 Lung Diseases Antibodies  >19 publications with IL25 and Lung Diseases
 Respiratory Hypersensitivity Antibodies  >14 publications with IL25 and Respiratory Hypersensitivity
 Hyperplasia Antibodies  >7 publications with IL25 and Hyperplasia
 Dermatitis, Atopic Antibodies  >7 publications with IL25 and Dermatitis, Atopic
 Necrosis Antibodies  >4 publications with IL25 and Necrosis
 Colitis Antibodies  >4 publications with IL25 and Colitis
 Liver Diseases Antibodies  >2 publications with IL25 and Liver Diseases
 Death Antibodies  >2 publications with IL25 and Death
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