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DDX18 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247547

MBS247547 is an antibody targeting DDX18. Also known as Rabbit Polyclonal to Human DDX18. Reactive in Human. Host: Rabbit. More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
DDX18, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human DDX18
Product Gene Name
Product Synonym Names
Anti-DDX18 Antibody (aa20-70) IHC-plus; DDX18; DEAD box protein 18; Myc-regulated DEAD box protein; MrDb; Human DDX18
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.05% BSA, 0.05% sodium azide
Purity/Purification
Protein A Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human DDX18
Preparation and Storage
Store at 4 degree C for short term applications. For long term storage, aliquot and store at -20 degree C.
Molecular Weight
75,407 Da
OMIM
606355
3D Structure
ModBase 3D Structure for Q9NVP1
Target Species
Human
Immunogen Description
A portion of amino acids 20-70 of human DDX18 was used as the immunogen.
Immunogen Type
Synthetic peptide
Immunogen
DDX18 antibody was raised against a portion of amino acids 20-70 of human DDX18 was used as the immunogen.
Antigen Modification
aa20-70

Product Categories/Family for anti-DDX18 antibody

Applications Tested/Suitable for anti-DDX18 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-DDX18 antibody
IHC-P (5 - 10 ug/ml), WB (2 - 4 ug/ml)

Immunohistochemistry (IHC) of anti-DDX18 antibody

Anti-DDX18 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml.
Rabbit Anti-DDX18 Polyclonal Antibody – Cat# MBS247547

Western Blot (WB) of anti-DDX18 antibody

Western blot ofDDX18 inT98G cell lysate in the 1) absence and2) presence of immunizing peptide using at3 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution were used for this test.
Rabbit Anti-DDX18 Polyclonal Antibody, Western Blot (WB) – Cat# MBS247547

Other Notes

Small volumes of anti-DDX18 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DDX18. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ATP-dependent RNA helicase DDX18
NCBI Official Synonym Full Names
DEAD (Asp-Glu-Ala-Asp) box polypeptide 18
NCBI Official Symbol
DDX18
NCBI Official Synonym Symbols
MrDb
UniProt Protein Name
ATP-dependent RNA helicase DDX18
UniProt Synonym Protein Names
DEAD box protein 18; Myc-regulated DEAD box protein; MrDb
UniProt Gene Name
DDX18
UniProt Synonym Gene Names
MrDb
UniProt Entry Name
DDX18_HUMAN
NCBI Protein Information
ATP-dependent RNA helicase DDX18; DEAD box protein 18; Myc-regulated DEAD box protein; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 18 (Myc-regulated)

NCBI Summary for DDX18

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it is activated by Myc protein. [provided by RefSeq, Jul 2008]
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UniProt Comments for DDX18

DDX18: Probable RNA-dependent helicase. Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.

Protein type: EC 3.6.4.13; RNA-binding; Helicase; Nucleolus

Chromosomal Location of Human Ortholog: 2q14.1

Cellular Component: membrane; nucleolus

Molecular Function: protein binding; ATP-dependent RNA helicase activity; ATP binding

Biological Process: metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DDX18 antibody

 Reference Product  PubMed Publications
 MYC antibody  >2 publications with DDX18 and MYC

Organs/Tissues associated with anti-DDX18 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1 publications with DDX18 and Blood
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