DRAQ5 Reagent – Supplied at 5 mM

Description: DRAQ5 is a novel far-red fluorescent DNA dye that can be used in live cells in combination with other common fluorophores, especially GFP & FITC-tags. DRAQ5 nuclear stain has many cellular analysis applications and is highly compatible with existing protocols across a wide range of instrumentation platforms.

Background: DRAQ5 far-red fluorescent DNA dye is a novel cellular imaging reagent for use in live cells or fixed cells in combination with other common fluors. DRAQ5 emits in the far-red (665 nm and beyond), making it ideal for use with GFP and all visible range-fluor tagged ligands. Due to this spectral separation, the GFP and nuclear signals can be captured simultaneously. For this reason alone, screening time is halved.

DRAQ5 far-red fluorescent dye directly and stoichiometrically reports DNA content in cells, live or fixed. Stained cells can be gated via flow cytometry and sorted into isolated populations with different DNA content relating to the cell cycle, such as elevated G2/M or aneuploidy. The DNA from the cell fractions can then be used directly for population-restricted PCR amplification. Because DRAQ5 is a far-red emitting DNA dye this can be easily multiplexed with other parameters, like cell surface phenotype.

DRAQ5 differentially stains both the nucleus and cytoplasm making it ideal for any HCS translocation (or Redistribution / Transfluor) assay. The intensity thresholding for the two compartments improves an in silico "dilation" while providing morphometrics which can be used to indicate toxicity. DRAQ5 has also been successfully used in HCS assays (e.g. GPCRs) measuring cell cycle, ploidy, or protein expression to gain speed, improve performance and efficiency.

Small volumes of DRAQ5 reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS258396 is a reagent targeting DRAQ5. Also known as DRAQ5 Far-Red Fluorescent DNA Dye.