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ASCC3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904250

Activating signal cointegrator 1 complex subunit 3 (ASCC3) is a 3'-5' DNA helicase involved in repair of alkylated DNA. ASCC3 promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3, enabling ALKHB3 to process alkylated… More details

$250 / 0.01 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
ASCC3, Polyclonal Antibody
Full Product Name
Rabbit anti-ASCC3 Antibody, Affinity Purified
Product Gene Name
anti-ASCC3 antibody
Product Synonym Names
RNA helicase family; helicase, ATP binding 1; trip4 complex subunit p200; RNAH; ASC1p200; ASC-1 complex subunit P200; activating signal cointegrator 1 complex subunit 3; HELIC1; Activating signal cointegrator 1 complex subunit 3; activating signal cointegrator 1 complex subunit 3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
614217
3D Structure
ModBase 3D Structure for Q8N3C0
Immunogen
Between 2152 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-ASCC3 antibody

Primary Antibodies
Applications Tested/Suitable for anti-ASCC3 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-ASCC3 antibody
WB: 1:500-1:2500 (3-8% Tris-acetate gel recommended)
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-ASCC3 antibody

Detection of human ASCC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-ASCC3 antibody MBS3904250 (lot MBS3904250-1) used for IP at 6 ug/mg lysate. ASCC3 was inefficiently immunoprecipitated by rabbit anti-ASCC3 antibody MBS3904249. For blotting immunoprecipitated ASCC3, MBS3904250 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-ASCC3 Polyclonal Antibody – Cat# MBS3904250

Western Blot (WB) of anti-ASCC3 antibody

Detection of human ASCC3 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-ASCC3 antibody MBS3904250 (lot MBS3904250-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-ASCC3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904250

Other Notes

Small volumes of anti-ASCC3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ASCC3 purchase

MBS3904250 is an antibody targeting ASCC3. Also known as Rabbit anti-ASCC3 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ASCC3. It may not necessarily be applicable to this product.

NCBI GeneID
10973
NCBI Accession #
NP_006819.2
UniProt Primary Accession #
Q8N3C0
UniProt Protein Name
Activating signal cointegrator 1 complex subunit 3
UniProt Synonym Protein Names
ASC-1 complex subunit p200; ASC1p200; Helicase, ATP binding 1; Trip4 complex subunit p200
Protein Family
Activating signal cointegrator 1 complex
UniProt Gene Name
ASCC3
UniProt Synonym Gene Names
HELIC1; ASC1p200
UniProt Entry Name
ASCC3_HUMAN

UniProt Comments for ASCC3

ASCC3: 3'-5' DNA helicase involved in repair of alkylated DNA. Promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3, enabling ALKHB3 to process alkylated N3- methylcytosine (3mC) within double-stranded regions. Enhances NF- kappa-B, SRF and AP1 transactivation. Belongs to the helicase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.12; Helicase; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 6q16

Cellular Component: Golgi apparatus; membrane; cytoplasm; intracellular; nucleus

Molecular Function: protein binding; ATP-dependent 3'-5' DNA helicase activity; ATP binding

Biological Process: cell proliferation; DNA dealkylation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; DNA duplex unwinding
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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